Temporospatial expression of fibulin-1 after acute spinal cord injury in rats

Abstract

Objective

Fibulin-1 is a matricellular protein that plays important roles in motility inhibition in a variety of cells and blocks the proliferation of cultured neural stem cells. The biological function of fibulin-1 in the spinal cord has not been fully elucidated.

Methods

To clarify the expressions and possible functions of fibulin-1 in spinal cord injury (SCI), we performed an acute spinal cord contusion injury model in adult rats. Our work studied the temporospatial expression patterns of fibulin-1.

Results

Western blot analysis revealed that fibulin-1 levels significantly increased 5 days after spinal cord contusion. Immunohistochemistry confirmed an increased number of fibulin-1 immunopositive cells about 2 mm from the lesion site. Moreover, double immunofluorescence labeling suggested that these changes were especially prominent in neurons and microglia.

Conclusion

These findings suggest that fibulin-1 may be involved in neuronal apoptosis and microglial activation after SCI.

Keywords:

• Fibulin-1
• Glial proliferation
• Neuron apoptosis
• Rat
• Spinal cord injury