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Abstract
A staining method for demonstrating spirochetes and bacteria, which stain poorly with the Gram stain, is described. The method is similar to the Warthin-Starry procedure except that the silver impregnation and reducing steps are done in a microwave oven and an acetic acid-glycine solution is used in place of citric acid. If spirochetes are to be stained, the sections are treated with uranyl nitrate, which is also heated in a microwave oven. The staining results are consistent and reliable. (The J Histotechnol 11: 149, 1988.)