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Abstract
The authors present a relatively simple autometallographical procedure for tnetal localization in biological tissues. The protocol demolistrates tnetals present at very low concentrations in certain key tissues of winkles atid mussels. The following procedural variables have been considered: the type of fixation, the type of photographic emulsion and its way of application, and the type of photographic developes. In addition, some staining techniques were used to contrast without masking revealed metal deposits by significant background levels. The protocol offers reproducible results and excellent resolution. This technique can valuable in pliysiological and toxicological studies on lnolluscs at the cell and tissue levels. (The J Histotechnol 21:123, 1998)
