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Abstract
There has been a continuous production of antibodies to lymphocytes that are immunoreactive in routinely fixed, paraffin-embedded sections so that the reliance for fresh tissue for the immunophenotyping of malignant lymphoid proliferations and their mimics is greatly reduced. Although antigen-retrieval procedures are necessary for the demonstration of many antigens, reliable immunophenotyping can be performed in fixed tissues for most lymphomas without resorting to frozen sections, cell imprints, and homogenized tissue preparations for flow cytometry. This article uses the panel approach for the immunotyping of the lymphoid proliferations using antibodies that are directed to diagnostic categories of tumors with common morphologic characteristics. Tumors are grouped according to cell size as large or small cell lymphomas and according to pattern as diffuse or follicular lymphomas. The immunophenotypic profile of Hodgkin's disease also is discussed. Finally, the properties and characteristics of useful antibodies for each of these categories of lymphomas are discussed in some detail. (The J Histotechnol 25:215, 2002)