Antifungal Activity of Amphotericin B and Itraconazole against Filamentous Fungi: Comparison of the Sensititre Yeast OneŴ and NCCLS M38-A Reference Methods

Abstract

The susceptibilities of 81 clinical isolates of Aspergillus spp.Fusarium spp., and Scedosporium spp., to amphotericin B and itraconazole were determined by the colorimetric microdilution method Sensititre® and the reference microdilution method of NCCLS standard M38-A for filamentous fungi. No major discrepancies were found and agreement ranged between 86.4% to 84% and 69.1% to 86.4% for amphotericin B and itraconazole respectively at 48 h and 72 h of incubation by using the recommended endpoints. Within two two-fold dilutions, high levels of agreement were found in general for amphotericin B at 48 or 72 h (86.4 to 87.7%) and itraconazole (91.4 to 93.8%). Relatively better agreement was found for itraconazole at 72 h of incubation and 48 for amphotericin B.

Keywords:

• Sensititre
• amphotericin B
• itraconazole
• susceptibility
• filamentous fungi