Lack of Effect of the Flavonoids, Myricetin, Quercetin, and Rutin, on Repair of H₂O₂;-Induced DNA Single-Strand Breaks in Caco-2, Hep G2, and V79 Cells

Abstract

In the present study the effects of three flavonoids on the repair of H₂O₂-induced DNA strand breaks were investigated in Caco-2, Hep G2, and V79 cells. At the concentrations used, myricetin, quercetin, rutin, and H₂O₂ did not significantly affect cell viability in all the cell lines. Catalase activity was measured in V79 cells and was found to be considerably lower than activities previously measured in Caco-2 and Hep G2 cells. Cells were exposed to 50 μM H₂O₂ for 0.5 hour at 37°C. After treatment, DNA strand break repair in H₂O₂-treated cells was monitored at various time points over a 48-hour period using the alkaline single-cell gel electrophoresis assay. Caco-2 cells repaired faster than Hep G2 cells, which repaired considerably faster than V79 cells. Preincubation with 50 μM quercetin for 24 hours significantly decreased the extent of H₂;O₂-induced DNA single-strand breaks throughout repair time points in Caco-2 cells (p < 0.05), but not in Hep G2 cells. Myricetin (50 μM) and rutin (50 μM) had no effect on repair in Caco-2 and Hep G2 cells. Preincubation for 10 hours with quercetin and rutin, but not myricetin, significantly decreased the initial extent of DNA damage induced by H₂O₂ in V79 cells (p < 0.05). However, from the results of this study, none of the three flavonoids increased the rate of repair of strand breaks in any of the cell types.