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Bioscience, Biotechnology, and Biochemistry Volume 74, 2010 - Issue 11
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Original Articles

Agrobacterium tumefaciens-Mediated Transformation of the Vegetative Dikaryotic Mycelium of the Cultivated Mushroom Flammulina velutipes

Tomomitsu OKAMOTODivision of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
,
Masato YAMADADivision of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
,
Shuichi SEKIYADivision of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
,
Toru OKUHARADivision of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
,
Goro TAGUCHIDivision of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
,
Satoshi INATOMIMushroom Research Laboratory, Hokuto Corporation
&
Makoto SHIMOSAKADivision of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
 show all
Pages 2327-2329 | Received 24 May 2010, Accepted 28 Jul 2010, Published online: 22 May 2014
 
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Abstract

Agrobacterium tumefaciens was used to transform the vegetative dikaryotic mycelium of Flammulina velutipes using a hygromycin B resistance gene as selectable marker. The gene coding for urogen III methyltransferase (cob) was introduced into F. velutipes dikaryotic cells. The resulting transformant cells generated a bright red fluorescence, indicating that cob is promising as a reporter gene in F. velutipes.

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Related Research Data

Current Technologies and Related Issues for Mushroom Transformation
Source: Informa UK Limited
Expression of asexual developmental regulator gene abaA is affected in the double mutants of classes I and II chitin synthase genes, chsC and chsA, of Aspergillus nidulans
Source: Springer Science and Business Media LLC
Cloning and characterization of a gene coding for a hydrophobin, Fv-hyd1, specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes
Source: Springer
pFungiway: a series of plasmid vectors used for gene manipulation in fungi
Source: Springer Science and Business Media LLC
Agrobacterium -mediated transformation of the filamentous fungus Aspergillus awamori
Source: Springer Science and Business Media LLC
Modern aspects of mushroom culture technology
Source: Springer Science and Business Media LLC
Enhancement of heterologous gene expression in Flammulina velutipes using polycistronic vectors containing a viral 2A cleavage sequence.
Source: Public Library of Science (PLoS)
Isolation and characterization of a gene coding for chitin deacetylase specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes and its expression in the yeast Pichia pastoris.
Source: Oxford University Press (OUP)
Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes.
Source: Mycobiology (KAMJE)
Agrobacterium-mediated genetic transformation of plants: biology and biotechnology.
Source: Elsevier BV
A Single Transcription Factor (PDD1) Determines Development and Yield of Winter Mushroom (Flammulina velutipes).
Source: American Society for Microbiology
Agrobacterium-mediated transformation of Fusarium oxysporum: An efficient tool for insertional mutagenesis and gene transfer
Source: Scientific Societies

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