Cloning and Characterization of a Chitosanase Gene from the Koji Mold Aspergillus oryzae Strain IAM 2660

Xiao-Yong ZHANGDepartment of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

An-Lan DAIDepartment of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

Kouji KUROIWADepartment of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

Ritsuko KODAIRADepartment of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

Masahiro NOGAWADepartment of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

Makoto SHIMOSAKADepartment of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

Mitsuo OKAZAKIGene Research Center, Shinshu University

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Abstract

A genomic copy of the gene coding for chitosanase (csnA) was isolated from Aspergillus oryzae IAM 2660. A. oryzae csnA contains an open reading frame that encodes a polypeptide of 245 amino acids with a calculated molecular mass of 26,500 Da. The deduced amino acid sequence of A. oryzae csnA indicates extensive similarities to those of other fungal chitosanases.

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Cloning and Characterization of a Chitosanase Gene from the Koji Mold Aspergillus oryzae Strain IAM 2660

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Cloning and Characterization of a Chitosanase Gene from the Koji Mold Aspergillus oryzae Strain IAM 2660

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