Protein–DNA Interactions under High-Pressure Conditions, Studied by Capillary Narrow-Tube Electrophoresis

Hiroaki KAWANOExtremobiosphere Research Center (XBR), Japan Agency for Marine-Earth Science and Technology (JAMSTEC);Department of Applied Chemistry, Faculty of Engineering, Toyo University

Kaoru NAKASONEDepartment of Biotechnology and Chemistry, School of Engineering, Kinki University

Fumiyoshi ABEExtremobiosphere Research Center (XBR), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

Chiaki KATOExtremobiosphere Research Center (XBR), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

Yasuhiko YOSHIDADepartment of Applied Chemistry, Faculty of Engineering, Toyo University

Ron USAMIDepartment of Applied Chemistry, Faculty of Engineering, Toyo University

Koki HORIKOSHIExtremobiosphere Research Center (XBR), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

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Abstract

The method of electrophoretic mobility shift assay under high-pressure conditions was improved using a high-pressure electrophoresis apparatus with capillary narrow-tube gel. It was found that the protein–DNA complex in the gel was stained as a high-resolution spot with ethidium bromide. Using this method, it was found that the behavior under high-pressure conditions of the protein–DNA complex composed of NtrC protein and its target promoter DNA is important for the pressure-regulated transcription process, and it was confirmed that the complex was dissociated above a pressure of 70 MPa.

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Protein–DNA Interactions under High-Pressure Conditions, Studied by Capillary Narrow-Tube Electrophoresis

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