Exosomes in diagnostic and therapeutic applications: biomarker, vaccine and RNA interference delivery vehicle

Abstract

Introduction: Cells release extracellular vesicles to their surroundings to communicate with each other. Exosomes are a subgroup of 30 – 100-nm-sized extracellular vesicles, originating from the endocytic pathway. They contain RNA molecules, proteins and lipids that can be transferred between cells. Exosomes have been found in several body fluids, indicating that this is a frequently used and tolerated system for cells to communicate RNA molecules and proteins over distances.

Areas covered: It has been shown that patients with cancer have higher concentrations of exosomes in their blood and that these exosomes can carry tumor-specific molecules. Exosomes are, therefore, currently being evaluated for their potential use as biomarkers. Additionally, exosomes have been demonstrated to have the capacity to modulate immune responses. Therefore, exosomes are believed to be beneficial as a cell-free vaccine for cancer and infections. Further, as exosomes are the body’s endogenous system for transport RNA, exosomes are also evaluated for their potential use as a therapeutic RNA delivery system. This review provides an overview of studies reporting diagnostic and therapeutic potential for exosomes.

Expert opinion: The data reviewed here suggest that exosomes have the potential to be used for both diagnosis and therapy for several diseases in the future.

Keywords:

• biomarker
• diagnosis
• drug delivery
• exosomes
• extracellular vesicles
• gene therapy
• small interfering RNA
• therapy
• vaccine

Acknowledgment

The author thanks A Cvjetkovic, GV Shelke, R Crescitelli and TR Lunavat for critically reviewing and editing the manuscript.

Declaration of interest

This work was supported by the Swedish Research Council, the Swedish Heart and Lung Foundation and VBG Foundation against Asthma and Allergy. The author has no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

Notes

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