881
Views
39
CrossRef citations to date
0
Altmetric
Reviews

Bioluminescence assays: multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay

, PhD & , PhD
Pages 835-849 | Published online: 21 Jul 2010
 

Abstract

Importance of the field: By selecting the most appropriate bioluminescence assay, the researcher can study the underlying molecular mechanisms of a physiological system and the effects of a drug throughout the body.

Areas covered in this review: This review covers three luciferase assay systems: the multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay. These assays are applied to drug screening in vitro, in cellulo and in vivo.

What the reader will gain: Different solutions for reporter assay in vitro, in cellulo and in vivo are presented. A suitable bioluminescence system depending on the assay purpose is also discussed.

Take home message: Bioluminescence is a manifold system based on the different types of luciferin and its luciferase. Namely, luciferin catalyzed by corresponding luciferases resulted in the production of different color lights. We must understand the manifold mechanisms of bioluminescence reaction.

Acknowledgments

We thank our colleagues who supported our research: K Honma, S Honma, E Ashitaka, S Itoh, M Ozaki, M Ikeda, C Wu, H Hoshino, K Niwa, K Yamagishi, T Watanabe, T Kimura, H Kubota, A Asakawa, T Enomoto, T Watanabe, S Nishii, T Yamazaki, N Ueda, A Oka and S Kumata.

Notes

This box summarizes key points contained in the article.

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 99.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 1,340.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.