Transport molecules using reverse sequence HIV-Tat polypeptides: not just any old Tat? (WO200808225)

Abstract

Background: Many polycationic cell penetrating peptides (CPPs), alternatively named protein transduction domains, have been used for the efficient intracellular delivery of biologically active agents. Patent WO2008/082885 relates to the properties and proposed biomedical applications of a CPP and putative-related sequences that represent the reverse sequence of a nonapeptide basic domain of the HIV-transactivator protein (Tat) (RRRQRRKKR). Objective: This evaluation critically assesses the reported utility of such transport molecules and the numerous potential embodiments of the invention, in comparison with other recent developments in the field. We also review recent biomedical applications of Tat-derived peptide transporters. Methods: The scope of this review includes both Tat-derived peptide transporters and other sequence-related CPPs that are polycationic in nature. Results/conclusion: The patent application indicates that reverse sequence HIV-Tat polypeptides can increase the transdermal delivery of an iodinated mixture of botulinum toxin, albumin and accessory proteins (Neuronox^®, Medy-Tox, Inc., Seoul, South Korea) as a non-covalent complex. Moreover, the invention also contemplates all variants of the reverse-sequence polypeptide and claims a variety of potential biomedical applications using the reverse sequence peptide as a delivery vector. Unfortunately, in the absence of both rigorous comparative data and toxicological analyses, it is uncertain if these transport molecules offer any advantages compared with many existing and rigorously characterised CPP vector systems.

Keywords::

• botulinum toxin
• cell penetrating peptide
• drug delivery
• HIV-1 transactivator protein
• protein transduction domain
• skin

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