Abstract
Evaluation of natural-origin, hatchery-origin, and unmarked steelhead Oncorhynchus mykiss from the Skykomish River drainage basin, Washington, was conducted to determine the source of unmarked steelhead that return to Sunset Falls (South Fork Skykomish River). One possible source is the large number of steelhead stocked into the Skykomish River basin from Reiter Ponds Hatchery; this hatchery stock was founded with fish from Skamania Hatchery in the Washougal River system, Washington. A microsatellite DNA analysis of 10 loci was used to evaluate unmarked samples in comparison with natural-origin samples from the North Fork Skykomish River and hatchery-origin samples from Reiter Ponds Hatchery. Results of the analyses provide evidence that the unmarked steelhead collected at Sunset Falls are more closely related to Reiter Ponds Hatchery fish than to natural-origin fish from the North Fork Skykomish River. There is evidence that unmarked steelhead at Sunset Falls are also mixing with natural-origin North Fork Skykomish River fish but to a lesser degree than with Reiter Ponds Hatchery fish. This study documents that Skamania Hatchery-origin steelhead have naturally produced offspring that are returning to spawn in a northern Puget Sound river basin.