Electron capture dissociation in the analysis of protein phosphorylation

Abstract

Protein phosphorylation is a widespread and important post-translational modification. Despite recent advances in phosphoproteomic methods, phosphopeptide identification and site localization remain challenging. Electron capture dissociation has inherent advantages for phosphorylation analysis. The use of electron capture dissociation in this area to date is reviewed and future prospects are outlined.

Keywords::

• bioinformatics
• CID
• ECD
• ETD
• FT-ICR
• mass spectrometry
• phosphoproteomics
• phosphorylation
• post-translational modification
• proteomics