Proteomics moves from expression to turnover: update and future perspective

Abstract

Proteomics is a rapidly developing discipline that seeks to understand the role of proteins in the wider biological context. In order to take a holistic view of a biological system, it is vital that we can elucidate the dynamics of the proteome. In this article, we have outlined the recent advances in experimental strategies for measuring protein synthesis and degradation on a proteome-wide scale. The application of mass spectrometry and non-mass spectrometric-based approaches in this field of research has been discussed. The article also explores the challenges associated with these types of analyses and the development of appropriate bioinformatic resources for interrogating the complex datasets that are generated.

Keywords::

• mass isotopomer distribution analysis
• mass spectrometry
• protein degradation
• protein synthesis
• proteomics
• stable isotopes

Financial & competing interests disclosure

The financial support of the Royal Society, Biotechnology and Biological Sciences Research Council, Research Councils UK, European Regional Development Fund, Highlands and Islands Enterprise and the Scottish Funding Council is gratefully acknowledged. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.