IDENTIFICATION OF CORYNEFORM ACTINOMYCES NEUII BY MALDI-TOF MS: 5 CASE REPORTS AND REVIEW OF LITERATURE

Abstract

Objectives: we describe five cases of Actinomyces neuii, isolated from different clinical specimens over a period of five months (from June to October 2011), followed by a review of literature on infections with this micro-organism.

Methods: all Actinomyces neuii strains were cultured or subcultured on horse blood agar. Identification took place using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS). Identification was confirmed by conventional biochemical tests and API Coryne test strips (BioMérieux^® SA). Susceptibility testing was performed on Mueller-Hinton agar supplemented with horse blood, using E-tests (BioMérieux ^® SA). The minimal inhibitory concentrations were determined after 24 and 48 hours of incubation in a 5% CO₂ environment.

Results: Isolation of this micro-organism was associated with abscesses in two patients and chronic osteomyelitis in one patient. The remaining two patients had positive blood cultures which grew Actinomyces neuii, either as contamination or as catheter-related infection. All Actinomyces neuii identifications were obtained by MALDI-TOF MS and were confirmed by conventional biochemical and API Coryne tests. Identification of one isolate was also confirmed by 16S rRNA sequencing. All strains were susceptible to penicillin. One strain showed heteroresistance for macrolides and lincosamides. Minimal inhibitory concentrations were more reliable and easier to read after 48 hours of incubation, as compared to 24 hours.

Conclusion: MALDI-TOF MS analysis allows rapid and reliable identification of Actinomyces neuii, even at subspecies level

Keywords:

• Actinomyces neuii
• MALDI-TOF MS
• identification
• susceptibility