Patient profiles and clinical utility of mepolizumab in severe eosinophilic asthma

Abstract

Mepolizumab (Nucala^®) is an effective and specific anti-eosinophil molecular therapy that has recently been approved as add-on therapy for the management of severe eosinophilic asthma by the US Food and Drug Administration (FDA), European Medicines Agency (EMA; European Union) and more recently National Institute for Health and Care Excellence (NICE; UK). It is one of several molecular therapies in development for this indication and is illustrative of the strategic trajectory for pharmaceutical drug development taken over the past decade in several disease areas. Molecular therapies offer the prospect of improved specificity and effectiveness of biological effect. However, this necessitates a clear understanding of the underlying mechanistic pathways underpinning pathological processes, to inform drug development that yields novel more efficacious treatment options with a better clinical profile than existing agents. For the first time, utilization of molecular therapies in clinical trials is providing a novel in vivo model to characterize the association between specific pathways and clinical disease expression. It is increasingly recognized that asthma exhibits both clinical and pathological heterogeneity. It follows that a one-size-fits-all approach will not be appropriate and cost-effectiveness may only be achieved by identifying responder subgroups. This so-called personalized approach to therapy is being supported by the parallel development of companion biomarkers for clinical application. In this review, the author summarizes the clinical studies, their interpretation and the lessons learnt with mepolizumab that have informed our understanding of the approach to personalized molecular therapy in asthma.

Keywords:

• IL-5
• Nucala
• exacerbations

Introduction

Refractory asthma, defined as a failure to achieve satisfactory asthma control despite adherence to high-dose inhaled therapies, comprises approximately 5% of the asthma population.¹ Despite this, it is estimated that 60% of asthma health care costs are attributable to refractory asthma² and more than 50% of asthma fatalities are recorded in patients with a prior history of severe disease.³ Morbidity is attributable to disabling symptoms, frequent severe exacerbations and the long-term iatrogenic sequelae of toxic immunosuppressive therapy, most notably oral corticosteroids (OCSs). The inadequacy of available therapies and the need for novel therapeutic targets are clear and have been at the forefront of asthma research over the past two decades.

The association between eosinophilic inflammation and asthma has long been recognized. Early necropsy studies^4,5 in patients dying from fatal asthma reported the identification of extensive eosinophilic inflammation and eosinophil degranulation (Charcot–Leyden crystals), accompanied by mucus hypersecretion and plugging of the small airways. Later, bronchoscopic studies demonstrated the association between asthma and eosinophilic inflammation in the airway lumen and sub-epithelial compartments of patients with milder asthma. Immunohistochemical studies identified eosinophil degranulation products and an association between the expression of CD25+ cells, elevated levels of interleukin (IL)-5 mRNA expression^6,7 and IL-5 protein^6,8 in biopsy material and bronchoalveolar lavage (BAL) fluid. Overall, these observations supported the view that CD4 T-cells releasing IL-5 orchestrate the eosinophilic response in asthma, with the eosinophil playing a key effector role in asthma pathogenesis. The clinical correlation between measures of asthma control and eosinophilic inflammation in blood and BAL has been reported in several studies,^9–11 while treatment with glucocorticoids to ameliorate eosinophilic inflammation in blood¹² and sputum¹³ has been associated with concomitant improvement in symptoms and lung function.

IL-5 belongs to a family of highly conserved and phylogenetically related growth factor cytokines that also includes IL-3, IL-4 and granulocyte monocyte colony-stimulating factor (GM-CSF). Genes coding for these proteins are closely linked on chromosome 5q in humans.¹⁴ IL-5 is uniquely positioned as the single most important cytokine modulating eosinophil hemopoiesis, homing and tissue survival (Figure 1). This provides an attractive pharmacological target for the development of novel anti-eosinophil therapies. In this context, it should be noted that while this review focuses on asthma, the development of “anti-IL5 therapies,” including mepolizumab, has a wider indication and potential role for the management of other eosinophil-driven disorders that include eosinophilic esophagitis,¹⁵ nasal polyposis,¹⁶ hypereosinophilic syndrome,¹⁷ Churg–Strauss syndrome¹⁸ and eosinophilic and atopic dermatitis.¹⁹ IL-5 is produced by CD4+ T-cells,²⁰ group 2 innate lymphoid cells (ILC2), mast cells and eosinophils themselves. Of these cell types, ILC2 appear to have a primary role in constitutive regulation²¹ while the others upregulate expression in response to an inflammatory stimulus. The IL-5R is a type 1 cytokine receptor, with a heterodimeric structure comprising separate α and β subunits. The α-subunit confers specificity, while the β-subunit is common to IL-5 and the hemopoietins, IL-3 and GM-CSF. Functional specificity of IL-5 arises from the restricted expression of its receptor (IL-5R), predominantly on eosinophils and to a lesser extent, basophils.²²

Figure 1 IL-5 and eosinophil biology.

Notes: Schema of factors regulating eosinophilopoiesis, homing and tissue persistence in disorders of eosinophilic inflammation. Although IL-5 is a key determinant in all three compartments of this model, other factors have an important role, particularly in eosinophil chemotaxis and tissue persistence.
Abbreviations: AM, alveolar macrophage; EC, extracellular; GM-CSF, granulocyte monocyte colony-stimulating factor; IL, interleukin.

Mepolizumab is a fully humanized neutralizing Ig G₁ antibody to IL-5 that sequesters the cytokine and prevents it binding with the α-subunit of the IL-5R. Preclinical studies conducted at the start of the millennium in cynomolgus monkeys²³ demonstrated the impressive anti-eosinophilic properties of the molecule. Significant and profound depletion of circulating and lavage eosinophils were observed after allergen challenge, with repression of blood eosinophil counts for 74 days after two doses of mepolizumab. The bio-availability and pharmacokinetic properties of intravenous (iv) and subcutaneous (sc) administration were comparable. Interestingly, these studies identified compartmentalization of drug distribution, with a fall in measured drug concentration from plasma to the airway and lung tissue that was accompanied by a concordant reduction in the effect on eosinophil counts. A similar gradient of effect has since been reported in humans and is discussed further in the following sections.

Clinical studies of mepolizumab in asthma are summarized in Tables 1–3, and for the purpose of this review are usefully considered in three phases: 1) an early development phase (Table 1); 2) a later development phase (Table 2) and 3) a validation phase (Table 3). Observations made in the studies of the early phase critically informed the scientific rationale and design principles for studies in the later development phase. This second phase successfully characterized the role of mepolizumab, and more generally eosinophils, in the clinical expression of asthma and was crucial for reviving the drug development program. The validation phase has provided robust evidence in larger and more diverse study cohorts to support the outcomes observed in development and inform biomarkers that predict a clinical response to therapy.

Table 1 Early Phase II clinical studies with mepolizumab

Study	Subject characteristics	Study objective	Study design	Primary outcomes	Secondary outcomes	Comments
Early studies (Phase II)
Flood-Page et al^Citation24: (N=24) Eosinophil’s role remains uncertain as anti-IL-5 only partially depletes numbers in asthmatic airways	Mild atopic asthma managed with as-required short-acting beta-agonist therapy alone	To evaluate the effect of mepolizumab on eosinophil numbers in blood, airways (BAL), bronchial mucosa and bone marrow To evaluate the effect of mepolizumab on morning PEF, His PC20 and FEV1	3 doses over 8 weeks: 750 mg iv/placebo	Change in eosinophil counts in different body compartments: • Near-complete suppression of blood eosinophil until 9 weeks after final mepolizumab dose • ↓79% in BAL • ↓55% in bronchial mucosa • ↓52% in bone marrow	Change in clinical parameters: PEF – no change FEV1 – no change His PC20 – no change	This study elegantly demonstrates the differences in the efficacy of mepolizumab for eosinophil suppression in different body compartments The lack of clinical effect with mepolizumab was attributed to the inability of the drug to completely suppress eosinophilic inflammation in the tissues
Flood-Page et al^Citation35: (N=362) A study to evaluate the safety and efficacy of mepolizumab in patients with moderate persistent asthma	Moderate persistent asthma: • On ≤1000 µg BDP equivalent per day • Persistent symptoms	To evaluate the role of mepolizumab for symptomatic asthma prior to escalating to high-dose corticosteroid therapy	3 doses over 8 weeks: 750 mg/250 mg iv/placebo	Morning PEF: no significant difference between groups	Change in FEV1 : no significant effect Asthma symptoms: no significant effect Blood eosinophil counts: significant reduction in blood eosinophils Exacerbation rates, categorized as mild, moderate or severe: no significant effect overall. Asthma quality of life: no significant effect Rescue albuterol use: no significant effect	Only major study conducted in an unselected asthma population Only study conducted in moderate asthma Only 53% of patients who had sputum induction performed had eosinophilia >3% There was a trend to significant reduction in severe exacerbations (p=0.06)

Note: His PC20, dose of histamine required to induce a 20% reduction in FEV₁ (AHR).

Abbreviations: AHR, airway hyperresponsiveness; BAL, bronchoalveolar lavage; BDP, beclomethasone dipropionate; FEV₁, forced expiratory volume in 1 sec; IL, interleukin; iv, intravenous; PEF, peak expiratory flow.

Table 2 Later Phase IIa clinical studies with mepolizumab Late studies (Phase IIa)

Study	Subject characteristics	Study objective	Study design	Primary outcomes	Secondary outcomes	Comments
Late studies (Phase IIa)
Haldar et al^Citation44: (N=61) Mepolizumab and severe exacerbation frequency in refractory eosinophilic asthma	Refractory asthma Sputum eosinophils >3% in previous 12 months ≥2 exacerbations in previous 12 months	First phenotype-specific study evaluating severe exacerbations as the primary end point	12 doses over 44 weeks: 750 mg iv/placebo	Annualized severe exacerbation frequency: ↓43%	Change in blood eosinophil count: ↓83% Change in sputum eosinophil count: ↓72% Change in ACQ-5: no significant effect Change in AQLQ: ↑0.55 PC20 Mch: no significant effect Change in FEV1 : no significant effect	Only study to perform serial sputum eosinophil counts Only study to characterize airway inflammation at the time of exacerbation: 36% of mepolizumab-treated subjects had a sputum eosinophilia >3% at exacerbation compared with 59% in the placebo group
Nair et al^Citation45: (N=20) Mepolizumab for prednisone-dependent asthma with sputum eosinophilia	Refractory asthma Sputum eosinophils >3% On maintenance OCS for ≥4 weeks at a dose of 5–25 mg	To evaluate whether mepolizumab could be used as oral steroid-sparing agent	5 doses over 16 weeks: 750 mg iv/placebo (5 doses over 16 weeks)	Proportion of patients having a severe exacerbation with OCS withdrawal: 1 out of 9 patients (mepolizumab treated) vs 10 out of 11 patients (placebo treated) OCS dose reduction achieved, expressed as a percentage of maximum possible dose reduction (protocol defined): 84% of maximal reduction (mepolizumab) vs 48% (placebo)		The greater reductions in steroid achieved compared with SIRIUS (see Table 3) may reflect the protocol which allowed steroid withdrawal to continue if an exacerbation was neutrophilic

Note: PC20 Mch, dose of methacholine to induce a 20% reduction in FEV₁.

Abbreviations: ACQ-5, Asthma Control Questionnaire (5-point score); AQLQ, Asthma Quality of Life Questionnaire; FEV₁, forced expiratory volume in 1 sec; iv, intravenous; OCS, oral corticosteroid; SIRIUS, Steroid Reduction with Mepolizumab Study.

Table 3 Phase IIb and Phase III clinical studies with mepolizumab

Study	Subject characteristics	Study objective	Study design	Primary outcomes	Secondary outcomes	Comments
Validation studies
Pavord et al^Citation50: (N=621) Mepolizumab for severe eosinophilic asthma: a multicentre, double- blind, placebo-controlled trial (DREAM)	Refractory asthma “and” ≥2 severe exacerbations in previous 12 months “and” blood eosinophils >300 cells/µL “or” sputum eosinophils >3% “or” FeNO >50 ppb “or” asthma deterioration with 25% reduction in ICSs	To validate outcomes observed in the study by Haldar et al^Citation44 To evaluate dose–response with mepolizumab for clinical outcomes To evaluate the effectiveness of different markers of the eosinophilic phenotype for predicting response to mepolizumab	13 doses over 48 weeks: 75 mg/250 mg/750 mg iv/placebo	Annualized severe exacerbation frequency: ↓39–52% across treatment arms No difference between doses	Exacerbations requiring hospital admission and ER visits: trend to significant reduction Change in FEV1 : no effect Change in AQLQ: no effect Change in ACQ-5: no effect Change in blood and sputum eosinophils: significant reduction in blood and sputum eosinophils with all mepolizumab treatment doses Dose-dependent reductions observed Change in FeNO: no effect	Study not powered to reliably assess the effect of mepolizumab on the number of hospital and ER episodes Multiple criteria for characterization of eosinophilic phenotype (exploratory) Modeling clearly identified that the efficacy of mepolizumab was dependent on both the baseline blood eosinophil count and number of severe exacerbations in previous 12 months
Ortega et al^Citation51: (N=576) Mepolizumab treatment in patients with severe eosinophilic asthma (MENSA)	Refractory asthma “and” ≥2 exacerbations in previous 12 months “and” blood eosinophils >150 cells/µL at screening “or” blood eosinophils recorded >300 cells/µL in previous 12 months	To evaluate and compare the mepolizumab treatment response between sc and iv routes	8 doses over 28 weeks: 100 mg sc/75 mg iv/placebo	Annualized severe exacerbation frequency: ↓47% with iv; ↓53% with sc	Change in: FEV1 ↑100 mL with iv; ↑98 mL with sc Change in SGRQ: ↓6.4 points with iv; ↓7.0 points with sc Change in ACQ-5: ↓0.42 with iv; ↓0.44 with sc	Equivalence of clinical effectiveness between iv and sc routes Improvements in lung function and symptoms as well as severe exacerbations Comparable profile of adverse events between iv and sc routes sc route associated with injection site reaction in 9% compared with 3% in iv group
Bel et al^Citation52: (N=135) Oral glucocorticoid-sparing effect of mepolizumab in eosinophilic asthma (SIRIUS)	Refractory asthma On maintenance OCS for ≥6 months at a dose of 5–35 mg Blood eosinophils >150 cells/µL during optimization “or” blood eosinophils recorded >300 cells/µL in previous 12 months	To evaluate the effectiveness of mepolizumab as an oral steroid-sparing agent	6 doses over 20 weeks: 100 mg sc/placebo	Percentage achieving dose reduction in maintenance OCS: OR of 2.39 in favor of mepolizumab to achieve a dose reduction in OCS	Median change in OCS dose: 50% reduction in median dose of OCS achieved (mepolizumab) vs no change (placebo) Annualized severe exacerbation frequency: ↓32% (mepolizumab vs placebo) Change in ACQ-5: ↓0.52 (mepolizumab) Change in SGRQ: ↓5.8 points Change in FEV1 : ↑114 mL	Almost half of subjects in each treatment arm had received OCS for >5 years This is the only study that demonstrates a beneficial effect of mepolizumab on asthma symptoms control OCS reduction decisions were based on symptoms rather than the eosinophil count – the eosinophil count, a better predictor of exacerbations, so OCS reduction decisions based on this end point may have achieved greater gains, as seen in the study by Nair et al^Citation45

Abbreviations: ACQ-5, Asthma Control Questionnaire (5-point score); AQLQ, Asthma Quality of Life Questionnaire; DREAM, Dose Ranging Efficacy And safety with Mepolizumab; ER, emergency room; FeNO, fractional exhaled nitric oxide; FEV₁, forced expiratory volume in 1 sec; ICS, inhaled corticosteroids; iv, intravenous; MENSA, Mepolizumab as Adjunctive Therapy in Patients with Severe Asthma; OCS, oral corticosteroid; OR, odds ratio; sc, subcutaneous; SGRQ, St. George’s Respiratory Questionnaire; SIRIUS, Steroid Reduction with Mepolizumab Study.

Early development phase for clinical studies of mepolizumab in asthma

Early clinical studies of mepolizumab offered a mixed picture. On the one hand, biological efficacy of the drug as an eosinophil suppressant was clear and consistently demonstrated; however, the studies failed to identify any improvement in the clinical end points that were measured. This discordance between clinical and biological effect was unexpected and prompted debate raising doubt for a meaningful role of eosinophils in asthma pathogenesis and, as a consequence, viability of the drug development program in asthma.

The immunobiological effects of mepolizumab in asthma were largely characterized by a series of reports arising from a single randomized, double-blind placebo-controlled study of three doses administered intravenously at monthly intervals to subjects with mild corticosteroid naive asthma²⁴ (Table 1). Treatment was associated with a 70% reduction in terminally differentiated bone marrow eosinophils²⁵ and a greater than 95% reduction in peripheral blood eosinophil counts. Consistent with the observations in cynomolgus monkeys, there was evidence for a gradient of efficacy across peripheral tissue compartments in humans, with mepolizumab achieving a reduction in eosinophil counts compared with placebo of 79% in lavage fluid and 55% in the bronchial submucosa. Despite the relative resistance of tissue eosinophils to therapy, significant effects of mepolizumab on airway structure were observed. Reticular basement membrane thickening, a hallmark feature of airway remodeling associated with eosinophilic airway inflammation in asthma, was significantly reduced, together with the reversal of pathological changes in the glycoprotein composition of the extracellular matrix. However, the study reported no difference of treatment compared with placebo on lung function (forced expiratory volume in 1 sec [FEV₁]) and airway hyperresponsiveness (AHR; AHR refers to the demonstration of a reduction in FEV₁ associated with inhalation of small doses of a bronchoconstrictor agent such as histamine. It is a hallmark of asthma. The dose of bronchoconstrictor agent required to achieve a 20% reduction in FEV₁ is used to quantify the degree of AHR) to histamine challenge.²⁶ These findings were consistent with an earlier placebo-controlled study evaluating a single dose–response of mepolizumab, administered at one of two doses (2.5 mg/kg or 10 mg/kg).²⁷ This study reported no effect of therapy on FEV₁, AHR and the late response to allergen challenge despite a significant reduction in blood and lavage eosinophil counts. Another study using an alternative anti-IL5 agent (SCH55700) in subjects with more severe, persistent asthma with impaired lung function also failed to show an improvement in FEV₁ with treatment.²⁸

A mechanistic explanation for clinical inefficacy was uncertain. It was argued that three doses of mepolizumab may be insufficient, and the time period too short, to observe changes in lung physiology.²⁴ On the other hand, the reduction in tissue eosinophils achieved with mepolizumab was comparable with the reported effect of high-dose OCS therapy,²⁹ which does significantly improve AHR and FEV₁ in asthma subjects with eosinophilic inflammation. In this context, two points are noteworthy: first, corticosteroids have a much broader spectrum of anti-Th2 activity that includes, but is not confined to, inhibition of eosinophilic inflammation^30,31 and second, anti-IL5 therapies promote uncoupling of eosinophilic inflammation from other Th2 processes (Figure 2). This idea is supported by the observations of a study by Büttner et al³² administering three doses of mepolizumab at monthly intervals to patients with moderate persistent asthma. Treatment was not associated with any effect on non-eosinophil leukocyte numbers, markers of T-cell activation, intracellular cytokine expression or cytokine receptor expression. It therefore follows that anti-IL5 therapies enable empirical classification of clinical variables as either being eosinophil driven and mediated (the eosinophil is an effector cell) or Th2 driven but not eosinophil mediated (the eosinophil is a biomarker). This represented a paradigm shift away from historical dogma that implicated the eosinophil as the primary effector cell in Th2-driven asthma pathogenesis and was supported by emerging evidence from other studies reporting dissociation between eosinophilic airway inflammation and AHR.^33,34

Figure 2 Adaptive immunity in asthma.

Notes: Both Th2 and Th1 pathways are believed to play a role in asthma-associated chronic airway inflammation. It is increasingly recognized that while either Th1 or Th2 processes may predominate, they are not exclusive. Key pro-inflammatory mediators such as TNFα have an important role in both pathways, enabling coexistence of inflammatory components from both pathways. Glucocorticoids have potent and broad-spectrum anti-Th2 activity. The effects of anti-IL-5 are more restricted. A comparison of clinical outcome between the two treatments offers the prospect of isolating the role of eosinophils from other Th2 factors.
Abbreviations: AM, alveolar macrophage; ASM, airway smooth muscle; DC, dendritic cell; IL, interleukin; MC, mast cell; TNFα, tumour necrosis factor - alpha.

In 2007, Flood-Page et al³⁵ reported on the first large-scale multicenter double-blind placebo-controlled clinical trial of mepolizumab in moderate persistent asthma (Table 1). The study enrolled 362 subjects with persistent symptoms despite treatment with inhaled corticosteroids (≤1000 µg beclomethasone dipropionate [BDP] equivalent per day) and randomized them to 3 months of iv treatment with either one of two doses of mepolizumab (250 mg or 750 mg) or placebo. Subjects were followed up and data collected for 8 weeks after the final dose. Of note, a subset of 32 subjects had sputum induction performed and 17 (53%) had evidence of eosinophilic inflammation. Although the study failed to show improvement in any clinical outcome measure despite significant reductions in the blood eosinophil count at both doses of active drug, a 50% reduction in severe exacerbations was observed during the follow-up period that failed to reach statistical significance (p=0.06) as the study was not powered for this outcome.

This review strengthened the view that traditional asthma outcome measures, notably morning peak flow, AHR and FEV₁ were unlikely to be eosinophil mediated. Furthermore, there was a growing body of literature describing non-eosinophilic asthma, a condition characterized by the absence of eosinophilic airway inflammation but with clinical and physiological features that are indistinguishable from eosinophilic asthma.³⁶ Observational studies suggested that approximately half of poorly controlled patients have a non-eosinophilic phenotype.^37,38 Typically, these patients have a poor response to corticosteroid therapy,³⁹ and no biological rationale for an anti-lL-5 therapy strategy. In keeping with this, 47% of participants that underwent sputum induction had an eosinophil count of <3%, suggesting a non-eosinophilic phenotype for a significant proportion of the cohort. The study was important for illustrating the perils of an unselected approach to subject enrollment, recruiting a significant non-responder group that would mask any beneficial effects of therapy. Despite this, it is notable that the study reported a trend toward reduction in severe exacerbation frequency that approached significance (p=0.06), supporting an effect of treatment on this clinical outcome for the subgroup of subjects that were likely responders. This was in keeping with a growing body of evidence from other sources implicating eosinophilic airway inflammation in the pathogenesis of severe exacerbations, including the previously described necropsy studies of fatal asthma;^4,5 studies reporting a rising sputum eosinophil count prior to and during exacerbations;⁴⁰ and studies demonstrating a significant reduction in exacerbation frequency achieved with a management approach titrating corticosteroid therapy to regular sputum eosinophil counts, independent of other measures of asthma control.^41–43

While the early clinical experience with mepolizumab was disappointing, significant gains were made in three key areas: first, the studies were reassuring for demonstrating biological efficacy; second, mepolizumab enabled a step change in our understanding of the relationship between eosinophilic inflammation and asthma pathogenesis and third, it became clear that translational studies of specific molecular therapies when applied to disorders such as asthma that express considerable pathological and clinical heterogeneity would need to be designed to be “phenotype specific” (directed to the subgroup of patients who are likely responders) and “outcome specific” (directed to end points that are likely to be associated with the biological effect of the therapy). These lessons critically informed subsequent studies and have almost certainly influenced the drug development program of other molecular therapies for asthma.

Late development phase for clinical studies of mepolizumab in asthma

In 2009, two independent small-scale single-center Phase II proof-of-concept studies were published that were the first to evaluate mepolizumab in asthma using a phenotype and outcome-specific approach (Table 2). The studies were complementary in their design and outcome and informed the design of subsequent larger phase IIb and Phase III studies. Haldar et al⁴⁴ investigated the effect of 12 doses of mepolizumab (750 mg iv given at four weekly intervals) on severe exacerbation frequency in 61 subjects with well-characterized refractory eosinophilic asthma and a history of recurrent severe exacerbations. In the study by Nair et al,⁴⁵ 20 subjects with refractory eosinophilic asthma requiring maintenance prednisolone therapy for control were randomized to evaluate the effect of five doses of mepolizumab (750 mg iv given at four weekly intervals) on the dose reduction in prednisolone achieved, titrated to the occurrence of severe eosinophilic exacerbations. There is a strong mechanistic rationale⁴⁶ for complementary effects of treatment with mepolizumab and OCSs in patients with severe eosinophilic asthma. In particular, blockade of IL-5 with mepolizumab lowers the number of circulating eosinophils and this synergises with the inhibitory effects of corticosteroids on eosinophil chemotaxis (suppression of eotaxins)⁴⁷ and eosinophil effector function (suppression of eosinophil cationic protein - a dominant eosinophil granule protein responsible for tissue damage).⁴⁸ Mepolizumab can therefore support successful corticosteroid dose reduction by contributing effectively to lowering levels of tissue eosinophilia.

Both studies used a targeted approach to recruitment, focusing on subjects with an eosinophilic phenotype of refractory asthma. Both studies also included severe exacerbations as the primary evaluation end point. The eosinophilic phenotype was defined in both studies by evidence of a sputum eosinophil count of greater than 3% on serial longitudinal measurement. In the study by Haldar et al,⁴⁹ the inclusion of a requirement for subjects to have had a history of recurrent severe exacerbations (two or more per year) is notable, following the group’s previously reported observation that the eosinophilic phenotype of asthma itself is heterogeneous, with a proportion of such patients having persistent eosinophilia but infrequent exacerbations. Thus, persistent eosinophilic inflammation confers a risk of severe exacerbations but does not predict that exacerbations will occur. It follows that the identification of recurrent exacerbations in the presence of eosinophilic inflammation would identify the subgroup of eosinophilic patients in whom the relationship between eosinophilic inflammation and exacerbation events is strongest, such that an anti-eosinophil strategy would have greatest impact on exacerbation prevention. In the study by Nair et al,⁴⁵ this subgroup was self-selected as all patients were prednisolone dependent on a clinical background of eosinophilic inflammation associated with poor control and recurrent exacerbations.

In contrast with the early clinical studies, mepolizumab demonstrated significant clinical efficacy for the primary end point of both of these trials (Table 1). In the study by Haldar et al,⁴⁴ mepolizumab was associated with a 43% reduction in severe exacerbations, compared with placebo, over 48 weeks. Consistent with earlier studies, the drug had no significant effect on symptom scores (Asthma Control Questionnaire [ACQ]), lung function (post-bronchodilator FEV₁) or AHR (methacholine PC20). Asthma Quality of Life Questionnaire (AQLQ) did improve and this was attributed to better exacerbation control. Biological efficacy was consistent with previous studies, with mepolizumab achieving an 83% greater reduction in blood eosinophil count compared with placebo over 48 weeks. The Haldar et al’s study remains the only study to have performed systematic serial sputum eosinophil count monitoring and reported a 72% reduction in sputum eosinophils compared with placebo over 48 weeks. In contrast with the relatively homogeneous blood eosinophil response to treatment, the suppression of sputum eosinophils with mepolizumab treatment was more heterogeneous. A significant correlation between the number of exacerbations and area under the curve sputum eosinophil count over 48 weeks was observed, implicating a causal role for airway eosinophils in the pathogenesis of exacerbations. In the study by Nair et al,⁴⁵ mepolizumab therapy was associated with a significantly greater reduction in prednisolone dose, expressed as a percentage of the maximum achievable per protocol reduction (84% vs 48%, Table 2). Ten of 11 subjects in the placebo arm experienced exacerbations with prednisolone withdrawal and nine of 12 exacerbations in this group were eosinophilic. In contrast, only one subject receiving mepolizumab had an exacerbation with steroid withdrawal and this was of a non-eosinophilic phenotype.

Overall, the observations of the studies by Haldar et al and Nair et al strengthen the view that mepolizumab, and probably specific anti-eosinophil treatment strategies more generally, are best suited to the management of eosinophilic patients with frequent severe exacerbations. For this phenotype, eosinophilic airway inflammation is a primary driver of exacerbation risk, such that effective suppression of this inflammation with treatment is associated with a corresponding reduction in severe exacerbations.

Validation phase for clinical studies of mepolizumab in asthma

The positive outcomes presented in the studies by Haldar et al⁴⁴ and Nair et al⁴⁵ provided sufficient evidence for investment into three larger Phase IIb and Phase III validation studies with the following objectives (Table 3):

1. Clinical: To validate that treatment with mepolizumab is associated with the clinical outcomes of a significant reduction in severe exacerbations and/or maintenance OCS dose in cohorts with an eosinophilic phenotype of asthma.

2. Pharmacological: To determine the dose, schedule and route of administration of mepolizumab achieving clinical effectiveness.

3. Phenotypic: To characterize biomarkers and patient characteristics that define the responder population for mepolizumab treatment.

These objectives have been supported by additional post hoc analyses of pooled study data. All three primary studies were conducted as multicenter, multinational, placebo-controlled double-blind parallel group clinical trials.

Objective 1a: validating the effectiveness of mepolizumab for lowering severe exacerbation frequency

Two of the validation studies – the Dose Ranging Efficacy And safety with Mepolizumab (DREAM) study⁵⁰ and Mepolizumab as Adjunctive Therapy in Patients with Severe Asthma (MENSA)⁵¹ – used severe exacerbation frequency as their primary clinical end point. Both studies selectively recruited patients with a likely eosinophilic phenotype and history of frequent severe exacerbations (≥2 per year). Treatment duration in DREAM was longer with subjects receiving 13 doses of treatment at four weekly intervals, compared with eight doses for MENSA.

The key difference between these studies helped to inform objective 2: DREAM was a Phase IIb study designed to compare the effectiveness of differing doses of iv therapy, while MENSA was designed to compare the effectiveness of mepolizumab administered at biologically equivalent doses administered by either the iv or sc route. In addition, the DREAM study helped to inform objective 3 by incorporating a breadth of criteria to identify the eosinophilic phenotype. These included either direct demonstration of eosinophilic inflammation (elevated blood or sputum eosinophil counts) or factors recognized to have an association with a Th2 high state (elevated fractional exhaled nitric oxide [FeNO] and symptom deterioration with lowering of inhaled corticosteroid dose).

Both DREAM and MENSA reported an approximate halving of annualized severe exacerbation frequency (39–52% rate reduction across different treatment subgroups) for mepolizumab compared with placebo that was entirely consistent with the reported effect in the study by Haldar et al.⁴⁴ Furthermore, DREAM identified no significant effect of mepolizumab on lung function (FEV₁), symptom scores (modified ACQ-5) or AQLQ. This was largely in keeping with Haldar et al, although Haldar et al⁴⁴ had reported an improvement in asthma quality of life that was attributed to the benefit of a lowering in exacerbation-related morbidity. In contrast, MENSA reported significant improvements in all three secondary outcome measures. Reasons for these differences are unclear; however, it is possible that any positive effect of mepolizumab on these outcome measures could be masked by improvements in these measures achieved by more frequent use of short-course corticosteroids to treat exacerbations in the placebo group. In this context, it is notable that the exacerbation frequency in the placebo arm of MENSA was lower (1.74 per year) than both Haldar et al (3.4 per year) and DREAM (2.4 per year).

Objective 1b: validating the effectiveness of mepolizumab for enabling dose reductions in maintenance OCS therapy

The Steroid Reduction with Mepolizumab Study (SIRIUS)⁵² was a Phase III study designed to validate the findings of Nair et al⁴⁵ for effectiveness of mepolizumab to achieve successful dose reductions in maintenance OCSs for patients with severe refractory eosinophilic asthma and oral steroid dependence. The study was of shorter duration than the other two Phase III studies and comprised treatment with six doses of either mepolizumab (100 mg sc) or placebo at four weekly intervals. Recruitment criteria included evidence of an eosinophilic phenotype and oral steroid dependence for a period of ≥6 months at a dose of between 5 mg and 35 mg. It is well recognized that OCS therapy is a powerful suppressant of the blood eosinophil count, yet the study investigators elected to identify the eosinophilic phenotype on the basis of a blood eosinophil count of either >300 cells/µL at any time in the preceding 12 months or >150 cells/µL during the study optimization phase – a period prior to randomization when subjects had their regular dose of OCSs systematically reduced at weekly intervals until they either had an exacerbation or their symptom scores increased by 0.5 points on the ACQ-5. The latter criterion would identify subjects in whom unmasking of underlying eosinophilic inflammation occurred with steroid withdrawal. Selection criteria to the study therefore favored recruitment of subjects with evidence of either relative steroid resistance or a potent eosinophilic drive. The inclusion of an optimization phase ensured that subjects had borderline asthma control at treatment baseline, enabling the “added value” of mepolizumab to be examined. There were some important differences between SIRIUS and the other mepolizumab studies that have been described. A prior history of frequent severe exacerbations, was not an inclusion criterion and in contrast with the study by Nair et al,⁴⁵ the protocol for steroid withdrawal during the treatment phase was based on clinical control alone and did not take into account eosinophilic inflammation. While practical, an implication of this approach was that the effectiveness of mepolizumab as a steroid-sparing agent relied on eosinophilic inflammation being closely associated with symptom control. This is contrary to the previously described observations of a dissociation between symptoms and eosinophilic inflammation. Indeed, in the study by Nair et al, it is this dissociation that provided the scientific rationale to continue steroid withdrawal (successfully) following an exacerbation when sputum induction identified an absence of eosinophilic airway inflammation. Despite this, SIRIUS reported an odds ratio of 2.4 in favor of mepolizumab for achieving a dose reduction in oral steroids and a 50% overall reduction in the median dose of OCSs, compared with placebo. Furthermore, the study reported significant improvements with mepolizumab in asthma symptoms, lung function and quality of life that were comparable with the observations in MENSA and consistent with the idea that these “softer” effects of mepolizumab are masked in an exacerbation directed study design that permits asthma control to be achieved in placebo subjects through the use of recurrent short courses of OCSs when presenting with poor clinical control. If this is accurate, then it suggests that eosinophilic inflammation contributes to asthma symptoms, though in contrast with severe exacerbations, is not the dominant factor.

Objective 2: determining the optimum dose and route of administration of mepolizumab for clinical application

As described earlier, both DREAM⁵⁰ and MENSA⁵¹ were designed to study this objective. Outcomes from DREAM demonstrated evidence of a dose–response effect on blood and sputum eosinophil counts, with increasing doses of mepolizumab leading to greater suppression of eosinophilic inflammation; however, this was not associated with a difference in any clinical outcome measures, compared with placebo. These observations suggest a non-linear relationship between clinical outcomes and eosinophilic inflammation, with the lowest dose of mepolizumab achieving the threshold of amelioration required for clinical control. In MENSA, there was no difference in clinical or biological effect, compared with placebo, between iv and sc mepolizumab given at doses of 75 mg and 100 mg, respectively. The biological efficacy of the sc route at a dose of 100 mg has been validated in a separate dose–response study that reported 74% bioavailability of mepolizumab via the sc route and 90% suppression of the blood eosinophil count at a dose of 99 mg.⁵³ Importantly, MENSA reported no excess of adverse effects with sc administration. As the favored route for administration in a clinical setting, the data support the use of mepolizumab 100 mg sc for clinical practice.

Objective 3: identifying biomarkers and patient characteristics that define the mepolizumab responder population

Characterization of the so-called responder phenotype requires an understanding of the biological mechanism underpinning clinical disease expression. For mepolizumab, it is clear that responders will be patients with eosinophilic asthma and a profile of clinical disease expression for which eosinophilic inflammation has a significant role. In this context, there is consistent evidence that the risk of severe asthma exacerbations in eosinophilic patients is closely associated with their level of underlying eosinophilic inflammation. A simple bivariate model for characterizing responders may therefore include a biomarker of eosinophilic inflammation, together with a clinical history of frequent exacerbations. While the latter is easily elicited from the patient’s history, there are a number of possible markers to measure eosinophilic inflammation.

Biomarkers of eosinophilic inflammation

The studies by Haldar et al and Nair et al utilized sputum eosinophilia as the marker of eosinophilic inflammation. As a disease of the airways, the sputum eosinophil count provides the most direct measure of eosinophilic inflammation that is anatomically pertinent and is generally considered to be the gold standard. However, sputum induction and processing requires expertise that precludes universal implementation.

The blood eosinophil count represents a steady state between the release of eosinophils from the bone marrow, an IL-5-driven process, and sequestration of cells in the tissues. There is evidence to support the view that asthma is associated with an increased sequestration of eosinophils in the lung parenchyma.⁵⁴ A peripheral blood eosinophilia in asthma is therefore a reliable measure of IL-5 upregulation and indicates a propensity for accelerated accumulation of eosinophils within lung tissue that may be reversed with mepolizumab therapy. Other biomarkers of the Th2 pathway (Figure 2) include FeNO⁵⁵ and serum periostin⁵⁶ and provide a surrogate measure of likely underlying eosinophilic inflammation as a correlate of Th2 activity.

The blood eosinophil count as a biomarker of mepolizumab response

As previously described, the DREAM⁵⁰ study included four different biomarkers to identify the eosinophilic responder phenotype. Of these, the baseline blood eosinophil count combined with the frequency of prior severe exacerbations was predictive in a modeling study of treatment response to mepolizumab, measured as the magnitude of relative reduction in exacerbations compared with placebo. It should be noted that sputum eosinophil counts were only available in a small subgroup of 94 participants (15% of the study cohort) and could not be included in the model. In a post hoc analysis of the DREAM and MENSA studies,⁵⁷ a positive correlation between the baseline blood eosinophil count and magnitude of treatment response with mepolizumab was identified, with a threshold baseline blood eosinophil count of 150 cells/µL predictive of significant treatment response. However, there is a caveat that this lower threshold was demonstrated for a carefully characterized eosinophilic population. As a blood eosinophil count to identify the responder subgroup within a heterogeneous asthma population, it lacks discriminatory power, falling well within the accepted normal range for blood eosinophils in the general population. In this context, it may be useful to consider two thresholds: 1) a higher blood eosinophil count (>300 cells/µL) to both reliably distinguish the responder population in an unselected cohort and inform mepolizumab treatment response; and 2) a lower threshold (>150 cells/µL) for considering mepolizumab therapy in cohorts with previously well-characterized eosinophilic asthma or, as in SIRIUS,⁵² for patients treated with regular OCSs, in whom suppression of the blood eosinophil count is expected.

Multidimensional phenotyping to characterize patient phenotypes of response

Multidimensional phenotyping using techniques such as cluster analysis provides a holistic and objective approach to identifying subgroups with shared characteristics within a heterogeneous population⁵⁸ and has been used extensively in characterizing asthma phenotypes.^49,59 In a post-hoc analysis of the DREAM study, cluster analysis was performed on the study population at baseline to identify responder subgroups.⁶⁰ Despite the use of targeted recruitment criteria, four clusters were identified with differing mepolizumab treatment responses. Of these, one cluster comprising 26% of the study population was characterized by low baseline eosinophil counts, high symptom burden and prior exacerbation frequency but a poor response to mepolizumab. Raised FeNO was the main criterion for recruitment to the study in this subgroup, suggesting that it is an unreliable surrogate marker of eosinophilic inflammation and mepolizumab responsiveness. This observation is in contrast with several studies that have demonstrated FeNO to be a reliable marker of steroid responsiveness and supports the view that a Th2 high state is not synonymous with eosinophilia.

The cluster demonstrating greatest response to mepolizumab in the analysis was a female-predominant obese, eosinophilic group with airway reversibility and a greater proportion requiring maintenance OCS treatment. Obesity has previously been reported to be associated with a non-eosinophilic phenotype that is typically unresponsive to steroids and would not be expected to be appropriate for mepolizumab treatment.^49,61 However, a recent study reported elevated sputum IL-5 and submucosal eosinophils but normal sputum eosinophils in obese patients with asthma.⁶² Whether this provides evidence for steroid resistance that is overcome with mepolizumab in the DREAM cohort is unclear.

Licensing of mepolizumab: indications for use in clinical practice

Mepolizumab received license authorization for use by the US Food and Drug Administration (FDA) in April 2015⁶³ and across the European Union (not the UK) in December 2015.⁶⁴ Both regulatory agencies approved the use of mepolizumab (100 mg sc at four weekly intervals) as add-on treatment in patients above the age of 12 years, with refractory disease, an eosinophilic phenotype and a history of exacerbations. The approved blood eosinophil count to determine an eosinophilic phenotype is >150 cells/µL, and no constraint has been placed on the number of prior severe exacerbations.

Mepolizumab has also recently received regulatory approval by the National Institute for Health and Care Excellence (NICE) in the UK.⁶⁵ However, stricter criteria are being applied with patient eligibility based on a blood eosinophil count of >300 cells/µL on one or more occasions in the preceding 12 months and either requiring treatment with maintenance OCSs and/or having a history of four or more exacerbations in the preceding year. The criteria are based on rigorous health economic analyses that report, when met, a cost per quality-adjusted life year (QALY) gained with mepolizumab of less than £30,000 is achieved if treatment is associated with at least a 50% reduction in exacerbation frequency. It is recommended that the decision to continue with treatment is based on an assessment of this outcome at the end of 12 months. A general overview of the clinical pathways to inform mepolizumab use in practice, based on the available evidence, is suggested in Figure 3.

Figure 3 Clinical profiling of patients to inform consideration of mepolizumab therapy.

Notes: ^aMarkers of Th2 inflammation (indirect measures of eosinophilic inflammation). ^bNICE recommends a review of response to treatment after 12 months. ^cThis is based on clinical judgment.
Abbreviations: NICE, National Institute for Health and Care Excellence; OCS, oral corticosteroid; sc, subcutaneous.

Adverse effects

Mepolizumab has been generally well tolerated in clinical studies to date. Commonly reported side effects include headache and back pain, although these symptoms did not occur at greater frequency in treated patients compared with placebo. Injection site reactions associated with subcutaneous administration are perhaps the most common treatment-related adverse effect.

Using mepolizumab in clinical practice: addressing the unmet need in refractory asthma

A recently reported observational study estimated approximately 20% of patients above the age of 12 years with uncontrolled asthma despite the use of high-dose inhaled corticosteroid therapy met US licensing criteria for mepolizumab, a figure that was comparable with the proportion eligible for omalizumab.⁶⁶ It is widely recognized that an eosinophilic phenotype is recognized in about 50% of patients with severe asthma. The review indicates that only a proportion of severe eosinophilic asthma would meet US licensing criteria. Consistent with this is the observation of Haldar et al⁴⁴ that only 33% of patients attending their severe asthma clinic met the eligibility criteria for their study.

The additional licensing constraints in the UK reflect a more conservative approach to regulatory control that is driven by cost. The NICE endorsed eligible population represents a cohort in which the amplitude of benefit justifies economic viability for a state-funded health service. However, it is likely that they comprise only a subgroup of the responder population and although it is clear that mepolizumab and similar therapies only partially address the unmet need in refractory asthma, optimizing their provision through the development of more predictive biomarker algorithms is an essential ongoing objective.

Outcomes after cessation of mepolizumab therapy

There are little published data of clinical and biological outcomes after mepolizumab treatment is stopped. A post hoc analysis of the Haldar et al’s⁶⁷ study cohort remains the only systematic evaluation of this question and reported significant increases in the blood and sputum eosinophil count 3 months and 6 months after stopping treatment with mepolizumab. Exacerbation frequency significantly increased after 6 months, in keeping with this end point being preceded by an increase in underlying eosinophilic inflammation. There was also a worsening of symptom control, with the ACQ-5 score increasing by 0.59 points over the 12-month period of observation. Supporting the view proposed earlier in this review that mepolizumab may have beneficial effects on symptom control that is masked by clinical trial design. A better understanding of the duration of beneficial clinical effects following prolonged therapy with mepolizumab and other comparable molecular therapies will likely be obtained from real-world clinical experience.

Conclusion

Mepolizumab is the first specific anti-eosinophil therapy to be licensed for use in the management of refractory asthma. It represents a paradigm shift in the therapeutic portfolio of this population. The developmental program for mepolizumab has illustrated many significant potential pitfalls that may only be avoided by a clear understanding of the relationship of disease pathobiology with clinical disease expression and using this information to characterize the patient profile most likely to benefit. For mepolizumab, this profile may be summarized as evidence of eosinophilic asthma that is associated with either a history of recurrent severe exacerbations or a requirement for maintenance OCS therapy.

The drug pipeline for refractory asthma is currently abundant with molecular therapies at various stages of development, and it is possible that mepolizumab may represent the first step toward a future without OCSs in asthma.

Disclosure

The author reports no conflicts of interest in this work.