https://orcid.org/0000-0001-8485-7172
Abstract
Purpose
COPD is a multisystem disease and there is a need for clinical serum markers that can assess the decline in lung and muscle function in COPD. The goal of this study was to evaluate the potential association of serum club-cell protein 16 (CC16), α-1 acid glycoprotein (AGP) and total sialic acid (TSA) with spirometry, hand-grip strength and quality of life to assess important disease outcomes.
Methods
This is a population-based cross-sectional study and data were collected from the patients at teaching hospitals of Gomal University and the University of Health Sciences in Pakistan. The study population included 1582 participants (Non-COPD; N = 788, COPD; N = 845) >55 years of age from both sexes, with data from structural interviews, clinical examinations, laboratory investigations, spirometry and hand-grip strength measurements.
Results
Serum TSA and CC16 were significant predictors of FEV1% (p < 0.05) and hand-grip strength in advanced stages of COPD (p < 0.05 each) in both sexes. Men had higher absolute and adjusted hand-grip strength than women in all groups (p < 0.05). Hand-grip strength was significantly associated with FEV1% in both genders (p < 0.05) with stronger effect in women (r2 = 0.075). Serum HDL-C was an independent predictor of hand-grip strength and FEV1% (p < 0.05) in both genders. Participants with extreme problem on EQ-5D parameters had more severe COPD and reduced hand-grip strength (all p values < 0.05).
Conclusion
Taken together, these studies show that the serum expressions of TSA and CC16 have correlations with spirometry and muscle decline in COPD. Further studies should be conducted to establish their efficacy in monitoring disease progression in COPD.
Introduction
Chronic obstructive pulmonary diseases (COPD) are frequently associated with systemic manifestations in addition to well-characterized progressive decline in the lung function.Citation1 The loss of muscle mass and strength in these patients is partly due to systemic inflammation and sedentary lifestyleCitation2,Citation3 which are strong predictors of mortality in the patients with COPD.Citation4 Hand-grip strength is a simple measure of upper limb muscles force and various respiratory and cardiovascular co-morbidities are associated with reduced hand-grip strength.Citation5 Several previous studies have elaborated a positive association between hand-grip strength and lung functions, assessed as the forced expiratory volume in 1 s (FEV1%) of the predicted value in the patients with COPDCitation6,Citation7 but the results have not always been consistent.Citation8 This is partly because the hand-grip strength, among other factors is affected by BMI,Citation9 serum metabolic profile including HDL-C,Citation10 smoking statusCitation11 and various co-morbiditiesCitation5 which are often overlooked when correlating hand-grip strength to disease severity in patients with COPD.
Several systemic biomarkers including club cell protein 16 (CC16), α-1 acid glycoprotein (AGP) and total sialic acid (TSA) have been associated with a decline in lung function in the patients with COPD.Citation12,Citation13 These serum biomarkers have shown promising association with FEV1% and/or hand-grip strength despite scarce data. Low levels of CC16, which is a secretory protein with anti-inflammatory properties are associated with greater decline in FEV1%Citation14 but its association with hand-grip strength in patients with COPD has not been elucidated, to our knowledge. AGP is an acute-phase protein and is reported to be a predictor of lung function in COPD,Citation15 but its association with hand-grip strength in the patients with COPD is not known. Sialic acid is a 9-carbon monosaccharide and is used as a marker of systemic inflammation in patients with COPD.Citation16 However, search for a serum biomarker to comprehensively characterize the association between FEV1% and hand-grip strength in patients with COPD remains elusive.
In clinical practice, physiologic tests like spirometry, hand-grip strength and self-reported health assessment tests have been used to evaluate disease severity in COPD.Citation17 EuroQol Five-Dimension Questionnaire (EQ-5D), a generic, health-related quality of life instrument has been used to assess the quality of life in patients with COPD.Citation6,Citation18
Based on these findings, we reasoned that the pathophysiological processes that occur in lung and skeletal muscle during COPD could be associated with expression of selected circulating biomarkers. More specifically, we aim to explore the relationship between serum CC16, AGP and TSA with clinically important outcome variables in COPD including FEV1%, hand-grip strength and quality of life. Furthermore, we aim to identify the clinical relevance of hand-grip strength by testing its association with FEV1% and EQ-5D in patients with various stages of COPD.
Methods
Study Design and Participants
Current study is a cross-sectional analysis of the baseline data collected from the selected participants with or without COPD at the University of Health Sciences, Lahore and the Gomal Medical College, Dera Ismail Khan. The regional ethical committees at both the universities approved this study. The study population included 1582 participants (Non-COPD; N = 788, COPD; N = 845, Men; N = 737, Women; N = 845) with age range from 56 to 75 years. Data were collected from structural interviews, clinical examinations, laboratory investigations, spirometry and hand-grip strength measurements over an eight-month time period starting January 2019. For statistical analysis, participants were divided into two groups with or without COPD. We used Global Initiative for Obstructive Lung Disease (GOLD) classification to further divide the COPD participants into 3 subgroups namely GOLD-1, GOLD-2 and GOLD-3 and 4. Written informed consent was obtained from all study participants. This study was conducted in accordance with the declaration of Helsinki.Citation19
Definitions
COPD was defined as FEV1%/forced vital capacity (FVC) < 0.7 with persistent respiratory symptoms according to the GOLD guidelines.Citation20 Only the patients with stable COPD were included which was characterized by absence of recent infections, hospitalization or exacerbation in past 1 month.Citation21 Among the patients with COPD, GOLD stage-1 was defined as FEV1 ≥ 80% predicted, GOLD stage-2 as FEV1 = 50% - 80% and GOLD stages-3 and 4 as FEV1 ≤ 50%. Body mass index (BMI) was calculated as kg/m2. Hypertension was defined as a systolic blood pressure ≥ 140mmHg or a diastolic blood pressure ≥ 90mmHg, and/or if antihypertensive medications had been prescribed. Diabetes was defined as fasting blood glucose levels ≥ 126 mg/dL or hemoglobin A1C ≥ 6.5% and/or treatment for diabetes. Smoking status was based on the use of tobacco products (eg, cigarettes, hookah or pipe) and the participants were classified into active smokers (smoked ≥ 1 tobacco product per day), ex-smokers (smoked ≥ 100 tobacco products over a lifetime and smoked at least once in the last 28 days) and non-smokers (smoked ≤ 100 tobacco products over a lifetime and did not smoke in the last 28 days). Subjects with stable COPD were included while those with unstable COPD (infection, exacerbation and/or hospitalization in the past 1 month), arthritis, myopathies and neurological diseases were excluded.
Hand-Grip Strength
Hand-grip strength was measured by a digital handgrip dynamometer (CAMRY, South El Monte, CA, USA). The participants were instructed to sit down with their elbows flexed at an angle of 90◦ with the dynamometer in hand in supine position. The participants were then asked to squeeze the dynamometer with maximal strength in a smooth manner without rapid jerking or wrenching. No other body movement was allowed during the procedure. Three attempts were performed with each hand with a 60-s rest between each attempt and the highest value was recorded for analysis.
Spirometry
The FEV1 and FVC were measured using a portable spirometer (Contec SP10, China), according to standards set by American Thoracic Society.Citation22 The participants were instructed to inhale maximally until the lungs were full, followed by forceful exhalation into the spirometer until no air could be exhaled.Citation23 This was done for a minimum of three times and the severity grading was based on FEV1% of predicted values according to the GOLD criteria into GOLD-1–4.Citation24 The quality of life was assessed using a 5-item self-administered, scale-based questionnaire (EQ-5D) which was developed by EuroQol group.Citation25 Only the descriptive section of EQ-5D was used which considers mobility, self-care, usual activities, pain/discomfort and anxiety/depression. Each parameter was assessed according to three functional levels: no problem, some problem or extreme problem.
Blood Metabolic Profile
Blood samples were drawn in the morning after 10–12 hrs of fasting to determine serum metabolic parameters including blood glucose and the high-density lipoproteins – cholesterol (HDL-C) using CardioCheck® equipment (Maxglobal SA, Parsippany, NJ, USA).
Measurement of Serum Biomarkers
For analysis of serum biomarkers, 18–20 participants from the non-COPD and each of the 3 subgroups of COPD participants were selected with equal representation of men and women. The numbers of participants per group or subgroup were selected based on the power (< 0.8) to detect 8–20% change in the serum levels of biomarkers at p < 0.05. Serum was assayed using ELISA kits for total sialic acid (abcam, cat # ab83375), club-cell 16 (Cat # RD19102200, BioVendor) and α-1 acid glycoprotein (abcam, cat # ab108852) according to manufacturer’s instructions.
Statistical Analysis
Anthropometric measurements of the participants were presented using mean and standard deviation as data met the assumption for normality. Analysis of variance was used to compare continuous variables and the chi-square test was used to determine categorical variables between the groups. Pearson correlation was employed to determine the strength of the relationship between hand-grip strength (dominant and non-dominant) and lung function (FEV1%, and FVC). A p-value < 0.05 was considered to be statistically significant. Hand-grip strength was adjusted for age, smoking status, waist circumference, anemia, hypertension and serum HDL-C in a multiple linear regression model using following equation
Results
Characteristics of the Participants
Basic characteristics of the study population are summarized in . Overall, the COPD participants had higher proportion of current and ex-smokers than the non-COPD participants. Participants with COPD also had higher proportion of co-morbidities including diabetes mellitus, hyperlipidemia, hypertension and anemia than the participants without COPD.
Table 1 Baseline Characteristics of the Study Participants
Hand-Grip Strength in COPD
Since hand-grip strength is influenced by age,Citation26 smoking status,Citation11 waist circumference,Citation27 anemia,Citation28 hypertensionCitation29 and serum HDL-C levels,Citation30 so we performed multivariable analysis to adjust for these factors. Among men, the adjusted hand-grip strength was 45.23 ± 4.11 for non-COPD group, 43.21 ± 3.71 for GOLD-1 subgroup, 41.88 ± 2.81 for GOLD-2 subgroup and 39.45 ± 3.75 for GOLD-3 and 4 subgroups (). Among women, the adjusted hand-grip strength was 30.31 ± 2.63 for non-COPD group, 29.01 ± 2.32 for GOLD-1 subgroup, 25.42 ± 2.41 for GOLD −2 subgroup and 23.82 ± 2.15 for GOLD-3 and 4 subgroups. Men had higher adjusted hand-grip strength (p < 0.05) than women among the non-COPD participants and three subgroups of COPD patients. Within the same gender, participants with GOLD-3 and 4 stages showed lesser adjusted hand-grip strength than the non-COPD participants among both men (39.45 ± 3.75 vs.45.23 ± 4.11; p < 0.05) and women (23.82 ± 2.15 vs 30.31 ± 2.63; p < 0.05) participants (). Further, among women participants, those with GOLD-2 stage also had lower adjusted hand-grip strength than the non-COPD group (25.42 ± 2.41 vs 30.31 ± 2.63; p < 0.05) ().
Figure 1 (A) Absolute and adjusted hand-grip strength for age, smoking status, waist circumference, anemia, hypertension and serum HDL-C in subjects with non-COPD and COPD by GOLD grades. Values are expressed as mean ± SD; one-way analysis of variance. * p < 0.05 vs women participants of the same group, # p < 0.05 vs non-COPD participants of the same gender. Relationship of the FEV1% with the absolute (B) and adjusted hand-grip strength (C) in the men and women participants with and without COPD.
Only women participants showed significant association between FEV1% and adjusted hand-grip strength (r2 = 0.005, p < 0.05) while the association between FEV1% and adjusted hand-grip strength failed to reach statistical significance in men (r2 = 0.001, p < 0.333) ().
Effects of Smoking Status on Hand-Grip Strength
Reduced hand-grip strength has been associated with smoking in different age groups.Citation11 To determine whether smoking affects hand-grip strength in the COPD participants, we measured hand-grip strength in the active, ex- and current smokers with COPD and adjusted it for co-variants. Among men, current smokers had lower hand-grip strength than the non-smokers of same age groups, as shown among the 55–65 years (41.27 ± 3.87 vs 44.65 ± 3.56; p < 0.05) and 66–75 years old (37.47 ± 4.21 vs 41.35 ± 4.32; p < 0.05) participants (). Among ex- and current smokers, 55–65 years old men had higher hand-grip strength than the 66–75 years old men (ex-smokers; 43.42 ± 4.32 vs 39.62 ± 3.51; p < 0.05, current smokers; (41.27 ± 3.87 vs 37.47 ± 4.21; p < 0.05). Statistical analysis was not conducted on female participants due to smaller sample size of ex- and current smokers.
Figure 2 (A) Hand-grip strength according to smoking status in the men and women participants of different age groups; Values are expressed as mean ± SD; one-way analysis of variance. * p < 0.05 vs 66–75 old participants of the same gender, # p < 0.05 vs non-smokers of the same gender and age group. (B) relationship of the serum HDL-C with the hand-grip strength and (C) FEV1% in men and women participants of the non-COPD and COPD groups. Hand-grip strength was adjusted for age, smoking status, waist circumference, anemia and hypertension in all analyses.
Relationship of Hand-Grip Strength and FEV1% with Serum HDL-C
An association of hand-grip strength and FEV1% with the serum metabolic parameters has been reported before.Citation10 In accordance with those findings, a significant association was found between the adjusted hand-grip strength and serum HDL-C in the male (r2 = 0.121, p < 0.05) and female (r2 = 0.085, p < 0.05) participants (). Serum HDL-C was also correlated to the FEV1% in the male (r2 = 0.113, p < 0.05) and female (r2 = 0.175, p < 0.05) populations ().
Relationship of Hand-Grip Strength and FEV1% with EQ-5D Score
Deterioration of health-related quality of life as assessed by EQ-5D score is generally associated with lower hand-grip strength.Citation31 In agreement with those findings, we report lower adjusted hand-grip strength in men and women participants with lower E1-5D score (). Participants with lower adjusted hand-grip strength and lower FEV1% had problems with mobility, self-care, usual activities, pain/discomfort, anxiety/depression than the participants with higher EQ-5D scores ().
Table 2 Classification of the COPD Participants According to EQ-5D Scores and Association with Hand-Grip Strength and FEV1%
Serum Biomarkers, Hand-Grip Strength and FEV1%
Among men, adjusted hand-grip strength was significantly correlated with TSA (r2 = 0.104, p < 0.05) () but not with AGP (r2 = 0.036, p = 0.155) () and CC16 (r2 = 0.035, p = 0.188) (). Among women, only CC16 showed significant correlation with adjusted hand-grip strength (r2 = 0.198, p < 0.05) () while of TSA (r2 = 0.039, p = 0.125) () and AGP (r2 = 0.002, p = 0.689) () did not show statistically significant correlations with adjusted hand-grip strength. TSA and CC16 were also strong predictors of FEV1% in men (TSA; r2 = 0.176, p < 0.05, CC16; r2 = 0.519, p < 0.05) and women (TSA; r2 = 0.112, p < 0.05, CC16; r2 = 0.569, p < 0.05) participants with COPD (). On the other hand, AGP was a poor predictor of FEV1% in both genders although it showed moderate correlation with FEV1% in men (r2 = 0.055, p = 0.072) (), which failed to reach statistical significance. In a group-wise analysis, serum TSA levels were elevated among participants with GOLD-3 and 4 (Men; ≈16.9%, p < 0.05, Women; ≈11.5%, p < 0.05) but not GOLD-1 or 2, when compared to non-COPD control group (). Similarly, serum CC16 levels were reduced among participants with GOLD-2 (Men; ≈29%, p < 0.05, Women; ≈29.6%, p < 0.05) and stages 3 and 4 combined (Men; ≈48%, p < 0.05, Women; ≈38.6%, p < 0.05), when compared to non-COPD control group. On the other hand, a significant change in the serum AGP levels was only found in the men with GOLD-3 and 4 stages when compared to the participants with GOLD-1 (≈29%, p < 0.05). Serum AGP levels in the women did not change among non-COPD participants and the three subgroups of COPD patients ().
Table 3 Protein Expression of the Serum Biomarkers of TSA, AGP, CC16 and HDL-C in the Sera of Participants with and Without COPD
Figure 3 Linear regression graphs of the hand-grip strength and FEV1% with serum biomarkers of TSA (A, B), AGP (C, D) and CC16 (E, F) in the men and women participants with and without COPD. Hand-grip strength was adjusted for age, smoking status, waist circumference, anemia and hypertension in all analyses.
Discussion
We report in this population-based cross-sectional study that: (1) a potential association exists between the circulating levels of CC16 and TSA and clinical variables of pulmonary (FEV1%) and muscle weakness in patients with COPD. (2) hand-grip strength has an association with spirometry and quality of life as participants with low FEV1% and low scores on mobility, self-care, usual activities, pain/discomfort and anxiety/depression consistently had lower hand-grip strength than the healthy controls.
Several groups have evaluated potential candidate biomarkers of COPD as a tool to monitor disease severity, suggesting that it might be possible to characterize COPD using systemic biomarkers.Citation32 However, the association of circulating biomarkers with muscle weakness in multi-systemic COPD is poorly characterized. Furthermore, the heterogeneity of COPD phenotype makes it difficult to draw definite conclusions from studies involving small sample size. Here, we investigated the biomarkers for decline in lung function and muscle weakness in patients with COPD, which will help to assess diseases progression during hospitalization and recovery stages of COPD.
Using a large sample size with considerable representation of patients with various stages of COPD, we have shown potential coupling of serum CC16 and TSA with FEV1% and hand-grip strength in COPD. The positive correlation between serum CC16 and FEV1% has been reported earlier.Citation33 Here, we have extended it to include hand-grip strength, at least in advanced stages of COPD. CC16 has anti-inflammatory and anti-toxicant effects in lungsCitation14 and may protect against spirometry decline in COPD. The positive association between CC16 and hand-grip strength reported in this study is probably because of anti-inflammatory properties of CC16, since systemic inflammation is known to reduce hand-grip strength.Citation34 We also found an association of serum TSA with FEV1% and hand-grip strength. TSA is a marker of inflammation and its serum levels are elevated in systemic inflammation associated with COPDCitation16 and sarcopenia.Citation35 However, to the best of our knowledge, no study has investigated the association between TSA and hand-grip strength in patients with COPD. In this study, we show the potential coupling of serum TSA with FEV1% and hand-grip strength in the participants with GOLD-2 and GOLD-3 and 4 who show significant weakness of hand-grip muscles.
The relationship between hand-grip strength and spirometry performance in patients with COPD is still controversial as an unchangedCitation6,Citation36 or reducedCitation37,Citation38 hand-grip strength has been reported with reduction in FEV1% in the COPD patients. This discrepancy is partly due to multiple factors including co-morbidities and inclusion of participants mainly with mild to moderate degree of COPD (GOLD-1 and 2 stages) in whom COPD may not be severe enough to trigger muscle weakness. Diabetes and hypertension have significant detrimental effects on muscle strength, which at least partly is due to increased secretion of inflammatory cytokines, motor neuropathy, elevated oxidative stress and lifestyle modifications. A small fraction of our healthy controls and patients with COPD had diabetes and hypertension. However, hand-grip strength was adjusted for these co-morbidities before the final analysis. Since the participants in each GOLD stage had a huge variation in the FEV1% values, we hypothesized that the FEV1% as a continuous variable might be a better predictor of hand-grip strength when evaluating disease severity in COPD. In support of this, we report an association between FEV1% and hand-grip strength in the COPD participants so that the reduced FEV1% was associated with lower hand-grip strength irrespective of GOLD stage. However, this coupling persisted only in women but not in men, when hand-grip strength was adjusted for confounders. Gender differences in the pulmonary function have been described before as women tend to have slightly higher FEV1% than men in reproductive age.Citation39 However, menopause is associated with an accelerated decline in lung functionsCitation40 and muscle strengthCitation41 because of low levels of estrogen and progesterone. Thus, menopause might have contributed to stronger coupling between FEV1% and hand-grip strength in women. Moreover, most of the women in our study were non-smokers, so additive negative effect of smoking on hand-grip strength was restricted to men only. Thus, after adjustment for this effect, the adjusted hand-grip strength did not show significant correlation with FEV1% in men, unlike in women. Thus, the reproductive aging and absence of smoking might have contributed to the stronger association between FEV1% and hand-grip strength in women, when compared to men.
Poor scores on mobility, anxiety, pain, discomfort and depression were consistently associated with reduced hand-grip strength in men and women with COPD. These findings are consistent with earlier reportsCitation31 and suggest that hand-grip strength can be used as a physical biomarker of the quality of life. Given that a direct coupling between health-related quality of life and disease severity in COPD participants has been proposed before,Citation42 measurement of quality of life in the COPD participants may be a useful tool to assess pulmonary decline and muscle weakness in COPD.
In terms of smoking affecting hand-grip strength,Citation11 reduced hand-grip strength in the male smokers is probably attributed to lung dysfunction as a significant direct effect of smoking on hand-grip strength in COPD is not well elucidated.Citation43 On the other hand, the relation between reduced HDL-C and reduced hand-grip strength has been described before in a list of diseases,Citation10,Citation30 which we expand to include COPD here.
A very large sample size and careful clinical, biochemical and functional characterization are the strengths of this study. However, there are several potential limitations. First, it was a cross-sectional study so we could not assess longitudinal changes in the hand-grip strength and serum biomarkers in relation to disease exacerbation and/or response to therapies. Second, we could not perform limbs' girth measurements and/or radiography (measures of muscle atrophy) and walk tests (functional measures of cardiopulmonary fitness); and hence could not analyze their correlations with hand-grip strength and serum biomarkers. Third, we could not perform computed tomography analysis of body composition and airways thickness which are recently shown to affect hand-grip strength independent of COPD.Citation44 Fourth, the number of participants with GOLD stages-3 and 4 in our study was much smaller than those with COPD stages 1 and 2, so caution should be exercised while interpreting the results. These proportions, nevertheless, represent the prevalence of COPD in the general population. Fifth, the number of smokers among women was very small for statistical analysis so our analysis on the correlation between smoking and hand-grip strength is restricted to men only.
In summary, we have shown that expressions of serum CC16 and TSA have positive associations with FEV1% and hand-grip strength in COPD. Smoking and lower serum HDL-C exacerbate the compromised muscle and lung functions in these patients. Further studies are required to establish the efficacy of serum CC16 and TSA for monitoring disease progression in COPD.
Data Sharing Statement
Raw data from the patient cohorts cannot be shared due to the privacy policies of the university hospitals from where data were collected.
Disclosure
The authors declare that they have no conflicts of interest in this work.
Acknowledgment
This work was supported by Seed grants from the University of Sharjah to Rizwan Qaisar (1901090146) and Asima Karim (1901090149). We are thankful to Maria Aziz and Jawad Ahmad for helping with data collection, Faisal Nadeem and Ahmad Hassan Nadeem for helping with data analysis.
References
- HuertasA, PalangeP. COPD: a multifactorial systemic disease. Ther Adv Respir Dis. 2011;5:217–224. doi:10.1177/175346581140049021429981
- WustRC, DegensH. Factors contributing to muscle wasting and dysfunction in COPD patients. Int J Chron Obstruct Pulmon Dis. 2007;2:289–300.18229567
- GosselinkR, TroostersT, DecramerM. Distribution of muscle weakness in patients with stable chronic obstructive pulmonary disease. J Cardiopulm Rehabil. 2000;20:353–360. doi:10.1097/00008483-200011000-0000411144041
- SwallowEB, ReyesD, HopkinsonNS, et al. Quadriceps strength predicts mortality in patients with moderate to severe chronic obstructive pulmonary disease. Thorax. 2007;62:115–120. doi:10.1136/thx.2006.06202617090575
- LeongDP, TeoKK, RangarajanS, et al. Prognostic value of grip strength: findings from the Prospective Urban Rural Epidemiology (PURE) study. Lancet. 2015;386:266–273. doi:10.1016/S0140-6736(14)62000-625982160
- JeongM, KangHK, SongP, et al. Hand grip strength in patients with chronic obstructive pulmonary disease. Int J Chron Obstruct Pulmon Dis. 2017;12:2385–2390. doi:10.2147/COPD.S14091528848339
- LeeSH, KimSJ, HanY, et al. Hand grip strength and chronic obstructive pulmonary disease in Korea: an analysis in KNHANES VI. Int J Chron Obstruct Pulmon Dis. 2017;12:2313–2321. doi:10.2147/COPD.S14262128831248
- Calik-KutukcuE, SavciS, SaglamM, et al. A comparison of muscle strength and endurance, exercise capacity, fatigue perception and quality of life in patients with chronic obstructive pulmonary disease and healthy subjects: a cross-sectional study. BMC Pulm Med. 2014;14:6. doi:10.1186/1471-2466-14-624468029
- LadUP, SatyanarayanaP, Shisode-LadS, Siri ChC, KumariNR. A study on the correlation between the Body Mass Index (BMI), the body fat percentage, the handgrip strength and the handgrip endurance in underweight, normal weight and overweight adolescents. J Clin Diagn Res. 2013;7:51–54. doi:10.7860/JCDR/2012/5026.266823450189
- LiD, GuoG, XiaL, et al. Relative handgrip strength is inversely associated with metabolic profile and metabolic disease in the general population in China. Front Physiol. 2018;9:59. doi:10.3389/fphys.2018.0005929459831
- Al-ObaidiS, Al-SayeghN, NadarM. Smoking impact on grip strength and fatigue resistance: implications for exercise and hand therapy practice. J Phys Act Health. 2014;11:1025–1031. doi:10.1123/jpah.2011-035723799259
- GhoshB. Serum biomarkers of COPD. Respir Care. 2016;61:395. doi:10.4187/respcare.04592
- KawamatawongT, ApiwattanapornA, SiricharoonwongW. Serum inflammatory biomarkers and clinical outcomes of COPD exacerbation caused by different pathogens. Int J Chron Obstruct Pulmon Dis. 2017;12:1625–1630. doi:10.2147/COPD.S13213228615935
- Laucho-ContrerasME, PolverinoF, GuptaK, et al. Protective role for club cell secretory protein-16 (CC16) in the development of COPD. Eur Respir J. 2015;45:1544–1556. doi:10.1183/09031936.0013421425700379
- EngstromG, SegelstormN, Ekberg-AronssonM, et al. Plasma markers of inflammation and incidence of hospitalisations for COPD: results from a population-based cohort study. Thorax. 2009;64:211–215. doi:10.1136/thx.2008.10207918988660
- RajendiranKS, AnanthanarayananRH, SatheeshS, RajappaM. Elevated levels of serum sialic acid and high-sensitivity C-reactive protein: markers of systemic inflammation in patients with chronic heart failure. Br J Biomed Sci. 2014;71:29–32. doi:10.1080/09674845.2014.1166995924693572
- RodriguesFM, DemeyerH, LoeckxM, et al. Health status deterioration in subjects with mild to moderate airflow obstruction, a six years observational study. Respir Res. 2019;20:93. doi:10.1186/s12931-019-1061-731103027
- PickardAS, WilkeC, JungE, et al. Use of a preference-based measure of health (EQ-5D) in COPD and asthma. Respir Med. 2008;102:519–536. doi:10.1016/j.rmed.2007.11.01618180151
- World MedicalA. World medical association declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA. 2013;310:2191–2194. doi:10.1001/jama.2013.28105324141714
- MirzaS, ClayRD, KoslowMA, ScanlonPD. COPD guidelines: a review of the 2018 GOLD report. Mayo Clin Proc. 2018;93:1488–1502. doi:10.1016/j.mayocp.2018.05.02630286833
- National Collaborating Centre for Chronic, C. Chronic obstructive pulmonary disease. National clinical guideline on management of chronic obstructive pulmonary disease in adults in primary and secondary care. Thorax. 2004;59(Suppl 1):1–232.
- CulverBH, GrahamBL, CoatesAL, et al. Recommendations for a standardized pulmonary function report. An official American thoracic society technical statement. Am J Respir Crit Care Med. 2017;196:1463–1472. doi:10.1164/rccm.201710-1981ST29192835
- ZaighamS, JohnsonL, WollmerP, EngstromG. Measures of low lung function and the prediction of incident COPD events and acute coronary events. Respir Med. 2018;144:68–73. doi:10.1016/j.rmed.2018.10.00730366586
- SafkaKA, WaldJ, WangH, McIvorL, McIvorA. GOLD stage and treatment in COPD: a 500 patient point prevalence study. Chronic Obstr Pulm Dis. 2016;4:45–55. doi:10.15326/jcopdf.4.1.2016.012628848910
- RabinR, de CharroF. EQ-5D: a measure of health status from the EuroQol group. Ann Med. 2001;33:337–343. doi:10.3109/0785389010900208711491192
- Massy-WestroppNM, GillTK, TaylorAW, BohannonRW, HillCL. Hand Grip Strength: age and gender stratified normative data in a population-based study. BMC Res Notes. 2011;4:127. doi:10.1186/1756-0500-4-12721492469
- OngHL, ChangSHS, AbdinE, et al. Association of grip strength, upper arm circumference, and waist circumference with dementia in older adults of the WiSE study: a cross-sectional analysis. J Nutr Health Aging. 2016;20:996–1001. doi:10.1007/s12603-015-0643-427925139
- JoostenE, DetroyerE, MilisenK. Effect of anaemia on hand grip strength, walking speed, functionality and 1 year mortality in older hospitalized patients. BMC Geriatr. 2016;16:153. doi:10.1186/s12877-016-0326-y27543049
- JiC, ZhengL, ZhangR, WuQ, ZhaoY. Handgrip strength is positively related to blood pressure and hypertension risk: results from the national health and nutrition examination survey. Lipids Health Dis. 2018;17:86. doi:10.1186/s12944-018-0734-429665844
- XuL, HaoYT. Effect of handgrip on coronary artery disease and myocardial infarction: a Mendelian randomization study. Sci Rep. 2017;7:954. doi:10.1038/s41598-017-01073-z28424468
- MarquesLP, ConfortinSC, OnoLM, BarbosaAR, d’OrsiE. Quality of life associated with handgrip strength and sarcopenia: epiFloripa aging study. Arch Gerontol Geriatr. 2019;81:234–239. doi:10.1016/j.archger.2018.12.01530654181
- KostikasK, BakakosP, PapirisS, StolzD, CelliBR. Systemic biomarkers in the evaluation and management of COPD patients: are we getting closer to clinical application? Curr Drug Targets. 2013;14:177–191. doi:10.2174/138945011131402000523256717
- GuerraS, HalonenM, VasquezMM, et al. Relation between circulating CC16 concentrations, lung function, and development of chronic obstructive pulmonary disease across the lifespan: a prospective study. Lancet Respir Med. 2015;3:613–620. doi:10.1016/S2213-2600(15)00196-426159408
- SmithL, YangL, HamerM. Handgrip strength, inflammatory markers, and mortality. Scand J Med Sci Sports. 2019;29:1190–1196. doi:10.1111/sms.1343330972827
- HaradaH, KaiH, ShibataR, et al. New diagnostic index for sarcopenia in patients with cardiovascular diseases. PLoS One. 2017;12:e0178123. doi:10.1371/journal.pone.017812328542531
- HallinR, JansonC, ArnardottirRH, et al. Relation between physical capacity, nutritional status and systemic inflammation in COPD. Clin Respir J. 2011;5:136–142. doi:10.1111/j.1752-699X.2010.00208.x21679348
- BurtinC, Ter RietG, PuhanMA, et al. Handgrip weakness and mortality risk in COPD: a multicentre analysis. Thorax. 2016;71:86–87. doi:10.1136/thoraxjnl-2015-20745126514408
- SueDY. Peripheral muscle dysfunction in patients with COPD: comparing apples to apples? Chest. 2003;124:1–4. doi:10.1378/chest.124.1.112853490
- LoMauroA, AlivertiA. Sex differences in respiratory function. Breathe (Sheff). 2018;14:131–140. doi:10.1183/20734735.00031829875832
- TriebnerK, MatulongaB, JohannessenA, et al. Menopause is associated with accelerated lung function decline. Am J Respir Crit Care Med. 2017;195:1058–1065. doi:10.1164/rccm.201605-0968OC27907454
- QaisarR, RenaudG, HedstromY, et al. Hormone replacement therapy improves contractile function and myonuclear organization of single muscle fibres from postmenopausal monozygotic female twin pairs. J Physiol. 2013;591:2333–2344. doi:10.1113/jphysiol.2012.25009223459759
- Medinas AmorosM, Mas-TousC, Renom-SotorraF, et al. Health-related quality of life is associated with COPD severity: a comparison between the GOLD staging and the BODE index. Chron Respir Dis. 2009;6:75–80. doi:10.1177/147997230810155119411567
- BoyerL, Bastuji-GarinS, ChouaidC, et al. Are systemic manifestations ascribable to COPD in smokers? A structural equation modeling approach. Sci Rep. 2018;8:8569. doi:10.1038/s41598-018-26766-x29872127
- MartinezCH, DiazAA, MeldrumCA, et al. Handgrip strength in chronic obstructive pulmonary disease. Associations with acute exacerbations and body composition. Ann Am Thorac Soc. 2017;14:1638–1645. doi:10.1513/AnnalsATS.201610-821OC29090990