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Review

Chitinases and Chitinase-Like Proteins in Obstructive Lung Diseases – Current Concepts and Potential Applications

, ORCID Icon & ORCID Icon
Pages 885-899 | Published online: 23 Apr 2020

Abstract

Chitinases, enzymes that cleave chitin’s chain to low molecular weight chitooligomers, are widely distributed in nature. Mammalian chitinases belong to the 18-glycosyl-hydrolase family and can be divided into two groups: true chitinases with enzymatic activity (AMCase and chitotriosidase) and chitinase-like proteins (CLPs) molecules which can bind to chitin or chitooligosaccharides but lack enzymatic activity (eg, YKL-40). Chitinases are thought to be part of an innate immunity against chitin-containing parasites and fungal infections. Both groups of these hydrolases are lately evaluated also as chemical mediators or biomarkers involved in airway inflammation and fibrosis. The aim of this article is to present the current knowledge on the potential role of human chitinases and CLPs in the pathogenesis, diagnosis, and course of obstructive lung diseases. We also assessed the potential role of chitinase and CLPs inhibitors as therapeutic targets in chronic obstructive pulmonary disease and asthma.

Background

Chitin is the second (after cellulose) most abundant biopolymer on Earth,Citation1 mainly forming various building block structures of fungi, mollusks, arthropods, and other invertebrates.Citation2 Its major unique features include water insolubility and high resistance to mechanical stress and degradation. The utility of chitin-containing products has been recognized and, since many years, chitin has been widely used for commercial purposes: in agriculture, food industry, pharmacy or biomedical industry.Citation3Citation5 Chitin is an enzymatic product of chitin synthases and requires specific enzymes – referred to as chitinases – for its degradation. Although it had previously been assumed that vertebral tissues contain no chitins and chitinases, in the light of recent studies, this assumption seems to be no longer valid.Citation6 Endogenous chitin can be found in some vertebrates, including fish and amphibians.Citation7 In general, human organisms are not able to produce chitin due to the absence of chitin synthases. However, minute amounts of chitin can be detected in humans. They either have external sources or may have the form of short-chain oligomers associated with hyaluronic acid synthesis.Citation6,Citation8 Thus, the lack of chitin synthases does not a priori exclude the presence of chitin in human tissues. It seems that under some circumstances even minute amounts of chitin or chitin-like polysaccharides can accumulate and contribute to human pathology, eg, in certain forms of Alzheimer’s disease.Citation9 Considering the high prevalence of chitin and the fact that the immunological reaction to this polysaccharide is thought to be time- and dose-dependent, the above issue seems to have at least potential clinical implications.Citation10 Nevertheless, the role of chitin in humans is still obscure.

Chitinases, enzymes that cleave chitin’s chain to low molecular weight chitooligomers, are widely distributed in nature. These enzymes are produced by bacteria, fungi, plants, actinomycetes, arthropods and vertebrates.Citation11 Curiously, albeit the human genome is completely void of genes for chitin synthases, it does contain several genes encoding different human chitinases.Citation12 Moreover, these genes are active and proteins with potent chitinolytic activity can be detected in different human tissues. This phenomenon is intriguing in the context of only a trace amount of chitin in human organisms. At least in part, chitinases probably represent a defensive mechanism against chitin-containing parasites and fungal infections.Citation13 In addition, these enzymes may also play a role in destroying potent chitin antigens. Nonetheless, it must be admitted that our current knowledge on the place of chitinases in human physiology and pathophysiology is highly unsatisfactory.

Human chitinases belong to 18-glycosyl-hydrolases (GH18). Within the family of GH18, we can distinguish two groups based on their activity:Citation14

  • enzymatically active chitinases, represented by chitotriosidase (CHIT1) and acid mammalian chitinase (AMCase);

  • chitinase-like proteins (CLPs), a group of several proteins which do not have hydrolytic activity but are still capable of binding chitin or chitin’s particles.Citation15Citation17 The most important representatives of this group are:

    • chitinase-3-like protein 1 (CHI3L1), also known as YKL-40 with animal homologue-BRP-39 (mouse breast regression protein 39);

    • chitinase 3-like protein 2 (YKL-39);

    • murine chitinase-like 3 protein (Ym1);

    • stabilin-1-interacting chitinase-like protein (SI-CLP);

    • oviductin.

Enzymatically active chitinases (true chitinases), ie, CHIT1 and AMCase, have been identified in different mammals including humans and laboratory animals (eg, mice). Humans and other mammals also produce YKL-40 (with mouse homologue BRP-39), oviductin and SI-CLP. Chitinase 3-like protein 2 (YKL-39) was found in humans but was absent in rodents.Citation18 Animal studies indicate the role of eating behaviors in chitinase gene expression. Higher AMCase enzyme and mRNA levels were found in omnivores (eg, pigs, chickens, monkeys) than in herbivores (eg, bovines) and carnivores (eg, dogs).Citation19,Citation20 The highest levels of chitinase expression were reported in the lungs, stomach, kidney, and liver.Citation19,Citation20 It may be hypothesized that a higher exposure to chitin-containing organisms results in the overexpression of chitinase genes.Citation21 It is worth to note, however, that chitinase genes are not always present in the genome (ie, according to the National Center for Biotechnology Information (NCBI) genome database, the CHIT1 gene is absent in the dog genome). The lack of chitinase gene in some species may lead to lower chitin digestibility and a reduced tolerance to overfeeding with chitin-containing food.Citation19

From the clinical perspective, CHIT1, AMCase and YKL-40 seem to play the most significant role in different human diseases. A common feature of the above enzymes is their potential involvement in the human immune response. Hence, the majority of studies have been focused on these molecules. These studies demonstrated various relationships between the level of CHIT1, AMCase or YKL-40 and different human diseases and pathologies, such as infections, cancer, chronic pulmonary disorders, arthritis, fibrosis, tissue remodeling, and others.Citation12,Citation14 The association of other CLPs with human diseases has been poorly addressed and both the number and the quality of data are limited, eg, YKL-39 was reported to be involved in osteoarthritis, whereas Sl-CLP probably plays a role in Th-2 induced allergies.Citation14 Since chitinases and CLPs are believed to play an active role in human immune defense, their activity should be expressed mainly in locations with high exposure to microorganisms. This refers to the lungs which are exposed to approximately 10,000 liters of air contaminated with various potential pathogens per day.Citation22,Citation23 Moreover, the most common diseases of the lower respiratory tract such as asthma and chronic obstructive pulmonary disease (COPD) have a strong relationship with inhaled antigens and air pollution. Therefore, the respiratory system seems to be one of the major sites in which the presence and activity of chitinases should be considered. The possible role of chitin, chitinases and CLPs in pulmonary diseases is shown in .Citation6,Citation18,Citation24 The aim of this article is to present the current knowledge on the potential role of human chitinases and CLPs in the pathogenesis, diagnosis, and course of obstructive lung diseases. In particular, two major aspects that are critical from a clinical perspective will be reviewed: their role in pathogenesis as a potential target of therapeutic intervention and the role as biomarkers characterizing specific features of the disease.

Figure 1 Schematic depiction of chitin, chitinases and chitinase-like proteins (CLPs) involvement in lung diseases.

Notes: Exposure to chitin may trigger the secretion of chitinases and CLPs from neutrophils, macrophages and other cells (solid-line black arrows show cell activation after chitin exposure and secretion of chitinases). Both groups of enzymes are thought to play a role in the pathogenesis of pulmonary diseases (eg, asthma and COPD). Black dashed arrows reflect a negative (ie, destructive) effect, while the grey dashed arrows show a possible positive (ie, protective) influence. See manuscript text for more details.
Figure 1 Schematic depiction of chitin, chitinases and chitinase-like proteins (CLPs) involvement in lung diseases.

Basic Characteristics of Selected Chitinases and CLPs Potentially Involved in Human Pathophysiology

Chitotriosidase

CHIT1 was the first true chitinase discovered in humans.Citation25 This enzyme has a hydrolytic and trans-glycosylation activity.Citation14 Elevated levels of CHIT1 have been first described in patients with Gaucher disease and later, in a variety of diseases (eg, acute and chronic infections, neurodegenerative diseases and other).Citation14,Citation25 In the human lung, CHIT1 is prominently expressed by alveolar macrophages and neutrophils and it is said to play an active role in the innate immune response.Citation26Citation28 Under normal conditions, the importance of CHIT1 results from its role in the defense against chitin-containing human pathogens, including fungi and insects.Citation28 CHIT1 may stimulate alveolar macrophages and enhance tissue inflammation.Citation27 However, the exact mechanism of CHIT1 action has not been fully explained.

Acid Mammalian Chitinase

In 2001, a new enzyme with chitinolytic activity – AMCase – was discovered.Citation29 It has been shown that AMCase has hydrolytic activity in the gastrointestinal tract and lungs and that it may play a role in digestion and/or immune defense.Citation30 The enzyme is acid stable, has an optimal pH around 2 and an acidic environment is required to initiate AMCase enzymatic activity.Citation31 This may suggest that the highest enzymatic activity can be found in gastric mucosa.Citation20 AMCase is expressed by lung epithelial cells and macrophages. AMCase mRNA expression and the number of AMCase-positive cells are upregulated during Th2 inflammation. This phenomenon has been demonstrated mainly in asthma (in lung epithelial and subepithelial cells) but also in chronic rhinosinusitis with nasal polyps (in ethmoid mucosa).Citation30,Citation32 The above effects were found to be mediated mainly through IL-13.Citation30,Citation33,Citation34 Although AMCase was detected in gastric mucosa, it did not correlate with the presence of H. pylori and inflammation.Citation35 The role of AMCase in the human respiratory system has not been adequately investigated and some authors suggested that this enzyme could be inactive in human lungs.Citation36

Chitinase-3-Like Protein 1

YKL-40, a 40 kDa plasma protein, is the best-known protein from the CLPs group. YKL-40 lacks chitinolytic activity due to the mutation of glutamic acid to leucine in the chitinase-3-like catalytic domain.Citation27 The expression of YKL-40 was found in different mammalian organs and tissues (ie, humans, cows, mice, goats).Citation37Citation40 The enzyme is secreted by macrophages, epithelial cells, neutrophils, chondrocytes, synoviocytes, vascular smooth muscle cells and cancer cells.Citation41,Citation42 Studies which used human lung specimens and bronchoalveolar lavage fluid (BALF) showed the expression of YKL-40 in the cytoplasm of neutrophils and macrophages both in healthy subjects and patients with obstructive airway diseases.Citation43,Citation44 Nevertheless, the role of this protein is somewhat controversial. It does not have any enzymatic activity but it is believed to be involved in the regulation of cell proliferation, adhesion, migration and activation.Citation14 Higher concentrations of YKL-40 in synovial fluid from patients with osteoarthritis and inflammatory joint diseases were reported already in 1993.Citation45 Later, elevated concentration of YKL-40 protein and mRNA were noted in many inflammatory diseases, malignancies and disorders associated with tissue remodeling (ie, pneumonia, inflammatory bowel disease, atherosclerosis, diabetes, liver fibrosis, and breast, liver, ovarian, colon or lung cancer).Citation46Citation50 To date, YKL-40 has been the most extensively evaluated chitinase in patients with obstructive lung diseases.

The Role of Chitinases and CLPs in Obstructive Lung Diseases

The knowledge on the pathogenesis of obstructive airway diseases has significantly evolved in the last decades but the mechanisms of airway inflammation and obstruction are still not completely understood. The role of chitinases and CLPs in asthma and COPD has been evaluated with the perspective that better understanding of the complex relationships between different mechanisms involved in the pathogenesis of these two diseases may become a prerequisite for the development of more personalized treatment and improvement of its effectiveness.

The Role of Chitinases and CLPs in Asthma

Asthma is a common airway disease characterized by a relatively specific type of chronic airway inflammation, airway hyperresponsiveness, and variable, reversible airflow limitation. According to the Global Initiative for Asthma (GINA) 2019 report, 300 million people worldwide suffer from this disease.Citation51 Untreated or inadequately treated asthma causes respiratory symptoms, reduces daily activity and quality of life, increases the risk and severity of exacerbations, including life-threatening events. Although the proportion of patients with severe asthma is rather small (usually reported between 3% and 8%), it is responsible for a significant proportion (20.6%) of total costs of treatment.Citation52Citation54 Therefore, a better understanding of the phenotype and endotype of severe asthma may not only improve the efficacy of treatment but also significantly reduce its cost.Citation55

The high prevalence of asthma and chronic cough among people exposed to chitin-containing compounds (eg, working in seafood or fungi manufactures) may suggest the importance of chitin in asthma pathogenesis.Citation56,Citation57 It is possible that chitin may act as an airborne endotoxin that triggers airway hyperresponsiveness. On the other hand, early exposition to chitin may also result in hypersensitization to chitin as an allergen.Citation58 These observations are consistent with the hygiene hypothesis, which emphasizes the importance of early childhood exposure to infections, microorganisms, and parasites in the proper development of the immune system. Thus, it has been hypothesized that a reduced number of infections with chitin-containing pathogens could contribute to the exaggerated Th2 response of the host which involves such Th2 cytokines as IL-4, IL-5 and IL-13 and may provoke hyperresponsiveness of the airways.Citation30,Citation59

There has been a number of human and experimental studies on chitinases and chitinase-like proteins in asthma. Human studies revealed that the levels of these proteins in asthmatics are higher than in control subjects. In most of the studies, chitinase levels were measured in serum and induced sputum.Citation60Citation62 Less commonly, BALF, and bronchial biopsies obtained by bronchoscopy or specimens from resected lung were used to measure chitinase activity and expression.Citation36,Citation64,Citation65

Chitinases and CLPs in Animal Models of Asthma

Several studies showed that AMCase plays a role in animal models of asthma. The results of these studies imply that the enzyme can be an important mediator of IL-13-induced response in Th2-related conditions.Citation30,Citation64 A significant increase of AMCase mRNA and protein was detected in the lung of ovalbumin (OVA)-sensitized mice (aeroallergen asthma model).Citation30 AMCase was significantly upregulated after 2 weeks of house dust mite (HDM) exposure in the murine model, while increased expression of this enzyme was not observed following cigarette smoke exposure.Citation65 The above data suggest that AMCase can be regarded as a marker of allergic airway inflammation.Citation65 HDM-induced mouse model of asthma was also used to evaluate the impact of selective AMCase inhibitor (compound 7f) on allergic airway inflammation. The study showed the effectiveness of this compound in reducing the number of inflammatory cells in BALF and total IgE concentration in serum. These changes correlated with the decrease of chitinolytic activity in BALF.Citation66

Another selective AMCase inhibitor also reduced allergic inflammation (eosinophilia) but its application was associated with unexpected serious side effects, including neutrophilia, an increase in macrophage inflammatory protein-1 alpha (MIP-1α) secretion and alterations in airway remodeling genes (resulting in increased procollagen I, matrix-metalloprotease-12 (MMP-12), and Ym1 production).Citation67 It has to be determined whether these serious adverse events were directly drug-induced or were the result of the altered eosinophil/neutrophil balance.

An animal study on the impact of CHIT1 on allergic asthmatic reaction led to some surprising conclusions.Citation68 The CHIT1-deficient mice demonstrated the enhanced allergic Th2 inflammatory, cytokine and IgE responses to OVA or HDM allergen sensitization and challenge. A possible protective role of CHIT1 in the pathogenesis of allergic inflammation and asthmatic airway responses is considered to be mediated by regulating transforming growth factor-beta (TGF-β) expression and Foxp3+ regulatory T cells (Treg).Citation68 BRP-39, an animal homologue of YKL-40, also plays an important role in the pathogenesis of IL-13-induced inflammation and tissue remodeling.Citation69 The involvement of this CLP in multiple immunological responses includes antigen sensitization, immunoglobulin E induction, stimulation of dendritic cell accumulation and activation, induction of alternative macrophage activation, inhibition of eosinophil, T cell, and macrophage apoptosis.Citation69

Chitinases and CLPs in Childhood Asthma

One study that included 322 children with asthma, 270 adult and 565 pediatric controls, demonstrated an association between polymorphism of genetic variants of AMCase and childhood asthma.Citation70 In contrast, mutations in CHIT1 did not seem to significantly affect asthma prevalence and severity.Citation71 Nevertheless, gene analysis of six children with severe asthma with fungal sensitization (SAFS) demonstrated that CHIT1 duplication may be a risk factor for SAFS, allergic bronchopulmonary aspergillosis and allergic fungal sinusitis.Citation72 An association between the serological features of fungal infection (fungal-specific IgG and IgA reactivity) and higher BALF chitinase activity was reported in asthmatic children.Citation73

Compared to non-asthmatics, children with asthma had increased YKL-40 levels in serum and BALF.Citation61,Citation73 Higher serum YKL-40 levels correlated with the fraction of exhaled nitric oxide, blood neutrophils, and bronchial wall thickening on high-resolution computed tomography.Citation61 Konradsen et al suggested that YKL-40 might be a risk factor of a worse course of the disease and resistance to standard therapy.Citation61 However, it must be stressed that other authors do not share this opinion.Citation74

Chitinases and CLPs in Adult Asthma

Numerous studies analyzed the role of YKL-40 in adult asthma. In general, the results of these studies suggested a relationship between the level of this enzyme and asthma, its severity and the level of asthma control.Citation44,Citation61,Citation75 Serum YKL-40 and CHIT1 were elevated in patients with asthma and their levels correlated with the degree of airway obstruction and the risk of exacerbation.Citation60,Citation76,Citation77 There was also a weak but statistically significant correlation between serum YKL-40 level and CHIT1 activity in patients with obstructive airway diseases.Citation60 Chupp et al found that patients with elevated serum levels of YKL-40 used significantly more rescue inhalers, required higher doses of oral corticosteroids and had a higher rate of hospitalization than patients with lower YKL-40 levels.Citation78 These authors also demonstrated an increased local expression of YKL-40 in bronchial mucosa in patients with severe asthma compared to the healthy control. Importantly, local YKL-40 expression correlated with serum YKL-40 level.Citation78 Other authors showed a significant increase in YKL-40 level in induced sputum (IS) after allergen bronchial provocation in patients with atopic asthma.Citation62

An interesting study on the relationship between serum levels of YKL-40 and a specific asthma phenotype was published by Specjalski et al.Citation44 The study included 167 patients with three different phenotypes of chronic asthma: early-onset atopic, late-onset non-atopic, and obesity-related asthma. The authors demonstrated significant differences between serum levels of YKL-40 in the three asthma phenotypes, with the highest YKL-40 levels in patients with the obesity-related disease. Serum levels of the enzyme were significantly higher in asthmatics with a high level of symptoms and exacerbations compared to patients with well-controlled disease as well as in atopic compared to non-atopic asthmatics.Citation44 The positive correlation between serum and sputum concentrations of YKL-40 in asthmatic subjects and truncal adiposity was confirmed by another study.Citation79 Moreover, the authors found that a high-fat diet augmented the pulmonary expression of YKL-40 (animal study). It was probably due to inflammatory, repair, and remodeling responses mediated by this CLP (probably partially mediated by interaction with Sirtuin 1).

A fascinating issue which may be, at least to some extent, linked to the relationship between severe asthma and high serum YKL-40 levels are the characteristics of inflammation associated with increased YKL-40 expression. Studies on sputum and serum YKL-40 level in asthma and COPD revealed a positive correlation with blood and sputum neutrophil count.Citation43,Citation80 At the same time, there were no correlations between chitinase concentrations (both YKL-40 and CHIT1) and blood eosinophils count either in asthma or in COPD patients.Citation60 This pattern of YKL-40 correlation with the number of neutrophils but not the number of eosinophils may suggest that neutrophils are an important source of YKL-40 in asthma.Citation43 On the other hand, as the correlation between the level of YKL-40 and some chemotactic factors for neutrophils (eg, IL-8) was reported, it might also be hypothesized that the increased number of neutrophils is an indirect effect of enhanced expression of YKL-40. Correlations between sputum YKL-40 and the levels of myeloperoxidase, IL-8, IL-6, and IL-5 in patients with severe asthma were observed by Hinks et al.Citation81 Additionally, Tang et al showed that YKL-40 induced IL-8 expression in bronchial epithelium via a mitogen-activated protein kinase (MAPK: c-Jun N-terminal kinases – JNK and extracellular signal-regulated kinase – ERK) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathways, causing bronchial smooth muscle proliferation and migration.Citation82 Considering the inability of corticosteroids to ameliorate neutrophilic asthma and neutrophil-generated overexpression of mediators aggravating airway remodeling [ie, TGF-β, IL-8, metalloproteinase 9 (MMP-9), elastase],Citation83Citation85 the correlation between YKL-40 and neutrophil cells in asthma is still uncertain and intriguing. All the above data seem to be consistent with clinical observations linking YKL-40 with severe, therapy-resistant asthma. However, it cannot be excluded that YKL-40 is only a marker of extracellular tissue remodeling. Whether YKL-40 is only an independent marker of airway inflammation or it is truly involved in asthma pathogenesis still needs to be elucidated. This requires a more comprehensive explanation of the exact mechanism of YKL-40 stimulation and action.

Of the three discussed chitinases, the most controversial data on the involvement in asthma pathobiology refer to AMCase. Zhu et al found that AMCase is expressed in lung tissue samples from patients with asthma but seems to be inactive in human lungs.Citation30,Citation36 Despite the fact that airway pH in patients with acute asthma is lower compared to healthy control (pH 5.23 vs pH 7.65),Citation86 the rate of airway acidification may be still insufficient for the optimum AMCase activity. The lack of AMCase bioactivity may, at least partially, result also from the increased pH in the airways during inhaled or systemic corticosteroid treatment.Citation30,Citation31,Citation86,Citation87 The lack or lower chitinolytic activity of human AMCase compared to the chitinolytic activity of mice AMCase may also result from evolutionary mutations of chitinase genes.Citation88 These processes lead to amino acid substitutions (some specific amino acids in human AMCase are not present in mice AMCase) and, probably, synthesis of a nonfunctional enzyme.

The Role of Chitinases and CLPs in COPD

Chronic obstructive pulmonary disease is another common chronic lung disease with high morbidity and mortality worldwide. It has been estimated that 328 million people suffer from COPD and according to the newest WHO report, it is the third leading cause of death worldwide.Citation89,Citation90 Airway inflammation, obstruction and remodeling, as well as structural changes in lung parenchyma belong to the most important features of COPD. These processes produce clinical symptoms (ie, dyspnea, productive cough, exercise intolerance) which are directly associated with irreversible and progressive airflow limitation, manifested particularly during disease exacerbations. COPD significantly reduces the quality of life and leads to physical disability. The main cause of COPD is tobacco smoke exposure and air pollution. Despite a significant progress in the understanding of the complex COPD pathophysiology, there are still many unresolved questions (ie, why COPD develops only in some smokers). In consequence, the effectiveness of treatment seems to be highly unsatisfactory.

An interesting concept of the relationship between COPD, chitin, and chitinases was proposed by Seibold et al.Citation36 These authors suggested that smoking is associated with higher airway exposure to chitin. This suggestion was based on the fact that tobacco leaves are commonly infected with fungal organisms.Citation36 Some years earlier, Verveij et al showed that commercially available cigarettes and marijuana (semi-legally sold in the Netherlands) were heavily contaminated with fungal spores.Citation91 Aspergillus fumigatus was the most frequently isolated organism from cigarettes, while Penicillium genus predominated in marijuana. Bearing in mind the above data, Seibold et al presented the hypothesis that polymeric chitin, present in cigarette smoke, might activate macrophages and airway epithelial cells and increase chitinase expression and secretion.Citation36

Studies evaluating the role of chitinases in the pathophysiology of COPD have been conducted since 2008Citation92 and include both a human and animal models. However, it should be emphasized that the number of publications on COPD is significantly smaller than on asthma.

Chitinases and CLPs in Animal Models of COPD

A study in the murine model of COPD showed that cigarette smoke (CS) exposure did not change the level of AMCase in the murine lung.Citation63 In contrast, an experimental study in the mouse model showed that exposure to CS led to higher YKL-40 accumulation in airway epithelial cells, alveolar macrophages, alveolar type II cells and an increase in YKL-40 mRNA concentration.Citation93 Importantly, the authors documented that YKL-40 was induced by an IL-18-dependent pathway. An immunohistochemistry study demonstrated that the overexpression of CHIT1 in a mouse lung pretreated with an adenoviral vector carrying full-length CHIT1 was localized mainly in the bronchial and bronchiolar epithelium and tissue macrophages.Citation27 CHIT1 overexpression promoted inflammation in the airways (as in COPD) manifested by a higher number of total cells, alveolar macrophages, and higher amounts of the murine homologue of IL-8 and monocyte chemoattractant protein 1 (MCP-1) in BALF.Citation27 Interestingly, the study by Matsuura et al demonstrated the protective role of YKL-40 against CS-induced lung injury.Citation93 The lack of this enzyme resulted in more pronounced CS-induced lung emphysema, mainly through the up-regulation of the CS-induced cell death response. Thus, the authors suggested that proper chitinase levels might play a protective role in preventing lung destruction.Citation94 These pleiotropic effects of chitinases (tissue destruction as a consequence of inflammation and remodeling and, on the other hand, the protective role against apoptosis) should be taken into consideration when planning therapeutic interventions targeted at chitinases (application of potential chitinase inhibitors).

Chitinases and CLPs in Patients with COPD

The involvement of chitinases in human COPD seems ambiguous. There were studies showing that AMCase probably plays no role in both patients with COPD and healthy smokers. The level of this chitinase in BALF was below the ELISA detection threshold.Citation27 On the other hand, serum, induced sputum (IS) and BALF levels of CHIT1 and YKL-40, as well as the number of CHIT1 positive cells infiltrating the bronchial epithelium and submucosa, were significantly higher in smokers with COPD than in smokers without COPD or never smokers.Citation27,Citation61,Citation62,Citation94,Citation95 It has been postulated that it could be related to higher macrophage and neutrophil CHIT1 and YKL-40 expression in COPD patients.Citation27,Citation36,Citation96 Recent studies showed that the release of CHIT1 is stimulated by interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), granulocyte-macrophage colony-stimulating factor (GM-CSF), lipopolysaccharide (LPS) as well as toll-like receptor (TLR) and nucleotide-binding oligomerization domain (NOD)-2 activation.Citation97Citation99 The exposure of alveolar macrophages from smokers with and without COPD to CHIT1 resulted in the production of IL-8, MMP-9 and MCP-1, cytokines said to be involved in tissue inflammation, alveolar destruction, and fibrosis.Citation27 Moreover, CHIT1 increases the activity of TGF-β signaling in fibroblasts (eg, through the induction of TGF-β receptor expression, inhibition of its feedback inhibitor – SMAD7 and interacting with a chaperone protein TGF-β receptor associate protein 1 (TGFRAP1) and forkhead box O3 (FOXO3)) resulting in enhanced production of extracellular connective tissue (eg, collagen), extracellular matrix protein (ECM) accumulation that may lead to alveolar destruction and tissue remodeling.Citation100Citation102 Possible CHIT1-related signaling pathways are shown in .Citation27,Citation36,Citation95,Citation97-102 It should be mentioned, however, that the exact role of CHIT1 in the pathogenesis of lung diseases is still highly hypothetical.

Figure 2 Schematic presentation of the hypothesized links between chitotriosidase (CHIT1) and the pathogenesis of lung inflammation and injury (eg, in COPD).

Notes: The exposure to cigarette smoke and other factors may enhance CHIT1 expression and increase its production, mainly by lung macrophages and neutrophils. The impact of CHIT1 on lung cells is heterogeneous and include inflammation and tissue destruction, mediated by interleukin 8 (IL-8), metalloproteinase 9 (MMP-9) and monocyte chemoattractant protein 1 (MCP-1), as well as lung fibrosis induced by transforming growth factor-beta (TGF-β) pathway. However, the exact role of CHIT1 in the pathogenesis of lung diseases is still highly hypothetical. A large white arrow represents stimulation of macrophages and neutrophils by various factors, the thick grey arrows show the secretion of CHIT1 from the cellular sources, the thin black arrows depict the pathways of CHIT1 activity, the vertical yellow arrow represents the increase or enhancement.
Figure 2 Schematic presentation of the hypothesized links between chitotriosidase (CHIT1) and the pathogenesis of lung inflammation and injury (eg, in COPD).

Similar pulmonary effect was demonstrated for YKL-40. Allergen and/or cigarette smoke (CS) exposure have been shown to trigger YKL-40 expression in several cell types (especially neutrophils, macrophages and epithelial cells).Citation69,Citation92,Citation93,Citation95,Citation96 The induction of YKL-40 expression and secretion may be mediated by various mediators, including interleukin (IL)-1,-13,-17A,-18 and TNFα.Citation65,Citation69,Citation95,Citation104Citation106 YKL-40 induces the production of proinflammatory and profibrotic mediators, ultimately resulting in tissue inflammation, alveolar destruction and remodeling. This complex process involves different pathways, mechanisms and multiple stages of inflammatory and tissue reactions. These include: 1) the activation and accumulation of dendritic cells (DC),Citation69 2) significant increase of IL-8 production from bronchial epithelial cells (dependent on MAPK and NF-κB pathway activation),Citation82 3) prevention of macrophage apoptosis and induction of alternative (M2) macrophage differentiationCitation69 with enhanced production of cytokines and chemokines, eg, IL-8, TGFβ, MCP-1, MIP-1α and MMP-9,Citation92 4) increased collagen production from lung fibroblasts (dependent on ERK and p-38 pathway activation).Citation96 Other inflammatory pathways may also play a role in modulation and aggravation of the YKL-40 response, eg, stimulation of TGFβ production and its activation by IL-13,Citation103 as well as IL-8 mediated proliferation and migration of bronchial smooth muscle cells.Citation82 The effects of YKL-40 on pulmonary structure and function are summarized in .Citation65,Citation69,Citation82,Citation92,Citation93,Citation95,Citation96,Citation103-106

Figure 3 Schematic representation of the supposed mechanisms involved in YKL-40-related emphysematous lung destruction, tissue inflammation and remodeling.

Notes: The large white arrow represents stimulation of macrophages, epithelial cells and neutrophils by various factors, the thin dashed black arrow depicts an indirect effect of environmental factors, the thick grey arrows show the secretion of YKL-40 from the cellular sources, the thin black arrows depict the possible YKL-40 signaling pathways, the thin dashed grey arrow depicts a stimulatory effect of interleukin 13 (IL-13) on transforming growth factor-beta (TGFβ), the vertical yellow arrow represents the increase or enhancement. See manuscript text for more details.
Figure 3 Schematic representation of the supposed mechanisms involved in YKL-40-related emphysematous lung destruction, tissue inflammation and remodeling.

Higher levels of CHIT1 and YKL-40 in serum and BALF of COPD patients correlated with a faster decline of lung function, the degree of airflow obstruction, the degree of diffusion lung capacity impairment, and frequency of exacerbations.Citation27,Citation94,Citation96 The results of other studies also emphasized a positive correlation between high serum YKL-40 concentrations and the severity of COPD (measured as the rate of decline in lung function, lower arterial oxygen pressure and desaturation during the 6-min walk test).Citation104,Citation107 Gumus et al showed a relationship between serum levels of YKL-40 and the low-attenuation area percentage (LAA%) in chest computed tomography in COPD patients, what may suggest the association between YKL-40 and development of emphysematous changes.Citation107 Interestingly, some studies suggest a protective role of CHIT1 against lung injury.Citation108,Citation109 Aminuddin et al found that smokers without a functional isoform of CHIT1 developed a faster rate of FEV1 decline.Citation108 This may indicate that an active form CHIT1 may play a protective role against rapid COPD progression. In fact, the relationship between CHIT1 and COPD refers rather to more profound mechanisms of the disease than to exposure to cigarette smoke and polymeric chitin, only. This might be supported by the observations on no relationship between the levels of CHIT1 chitinolytic activity in BALF and serum with smoking history (measured in pack-years).Citation60,Citation98

The possible protective role of chitinases in inflammation and lung injury may, at least partially, be related to anti-inflammatory effects of CHIT1 (mentioned earlier in the section on animal models of asthma).Citation68 CHIT1 inhibits allergen-stimulated Th2 cytokine production while enhancing the expression of the anti-inflammatory cytokines (TGF-β1 and IL-10).Citation68 Moreover, CHIT1 enhances TGF-β1–stimulated fibroblast proliferation and ECM accumulation in lungs.Citation100 An optimal level of CHIT1 seems to be both necessary and sufficient for physiologic TGF-β1–stimulated fibrotic ECM deposition and tissue repair responses. Nevertheless, the protective and favorable role of chitinases in inflammation and lung injury seems to be somewhat controversial and the data supporting this view are uncertain.

The Impact of Sex and Age on Chitinase and CLP Levels in Various Biological Samples

The activity of chitinases and their levels should also be discussed in the context of sex and ageing. Several studies documented a relationship between serum level of YKL-40 as well as CHIT1 and age. These findings refer to both patients with obstructive airway diseases (asthma and COPD) and healthy individuals.Citation60,Citation110,Citation111 The increase in chitinase levels and activity in the elderly population could be associated with age-related comorbidities. It is also possible that the higher serum chitinase activity in older patients is associated with the accumulation of lipid-laden macrophages during the progression of age-related atherosclerosis.Citation111 In contrast to age, no differences between plasma YKL-40 levels in healthy men and women were found.Citation110

The Polymorphisms of Chitinases/CLPs and the Course of Obstructive Airway Diseases

There were some studies aimed to evaluate whether the genetic variations of chitinase genes affect the course of obstructive diseases. Most of these studies analyzed the potential role of chitinase polymorphism (single-nucleotide polymorphisms – SNPs) in the expression of chitinase proteins and lung function.Citation70,Citation112,Citation113 Some authors found that SNPs in AMCase and YKL-40 gene influence chitinase levels and have been associated with asthma status, atopy, IgE levels and impaired lung function.Citation70,Citation112,Citation113 However, other studies showed no relationship between the polymorphism of chitinases and CLPs (AMCase, chitotriosidase and YKL-40) and asthma or allergy.Citation71,Citation114 One possible explanation for these discrepancies may be associated with differences in cohort characteristics included in various studies. It is possible that the influence of variation of these genes alone is weak and gene–environment interactions are essential to provoke allergic reactions. As mentioned previously, a 24-bp duplication in exon 10 in the CHIT1 gene results in producing a nonfunctional form of the protein.Citation36 This polymorphism has been associated with reduced chitinase activity in the lungs of smokers. The results of the analysis of genetic variations of CHIT1 and AMCase genes in large cohort groups are inconsistent. Aminuddin et al demonstrated that polymorphisms of CHIT1 and AMCase were associated with the baseline FEV1 in a large cohort of COPD patients.Citation108 Moreover, there was a relationship between CHIT1 rs2494303 and AMCase G339T genotypes and a faster rate of lung function decline. Due to the differences in demographic factors characterizing different study cohorts (ie, the LHD group was statistically younger than the subjects in other cohorts), the authors suggested that chitinase polymorphism probably plays a role in lung development or early impairment of lung function but its influence in later stages of COPD (eg, as an effect of multiple factors that influence the course of the disease) cannot be easily estimated.

The Effect of Corticosteroids on Chitinase and CLP Levels

The impact of corticosteroids on the levels of chitinases and CLPs seems to be an important and interesting issue. This is not only because corticosteroids are commonly used to treat asthma and COPD but also because some intriguing data on the influence of systemic corticosteroids on chitinase levels in patients with different diseases have been reported. As early as in 1999, Johansen et al reported that prednisolone therapy reduced serum YKL-40 levels in patients with rheumatoid arthritis.Citation115 On the other hand, the BIOAIR study showed that two-week oral prednisone course (0.5 mg/kg body weight/day) added to regular treatment did not change serum YKL-40 levels and chitotriosidase activity in patients with asthma.Citation60 Surprisingly, the same therapeutic regimen in patients with COPD resulted in a reduction in serum chitotriosidase activity.Citation60 Moreover, an in vitro study by Kunz et al revealed that dexamethasone strongly inhibited the release of YKL-40 from the population of proinflammatory macrophages that are characterized by classic mechanism of activation, secretion of pro-inflammatory cytokines (eg, IFN-γ, IL-1, IL-6, IL-12) and promotion of Th1 immunity.Citation116 However, long-term treatment with ICS (30 months) did not significantly change sputum and serum YKL-40 levels in patients with COPD.Citation116 The lack of change in serum YKL-40 level may result from a low circulating corticosteroid level during ICS treatment.Citation117 In addition, there are data suggesting that lung macrophages from patients with COPD have reduced corticosteroid sensitivity compared with macrophages from smokers without COPD.Citation118 Thus, it seems essential to elucidate whether YKL-40 and other chitinase gene expressions are directly controlled by corticosteroids.

Perspectives

To date, chitinases and CLPs were mainly evaluated as chemical mediators or biomarkers involved in airway inflammation and fibrosis (as shown in ). Some data presented in the paper point to their role not only in airway immunopathology, including chronic inflammation and tissue remodeling,Citation27,Citation30,Citation119 but also in the clinical course of asthma and COPD, ie, severity of airway obstruction, rate of exacerbations and response to steroid treatment.Citation60,Citation61 It might be supposed that future studies will be focused not only on the role of chitinases and CLPs in airway diseases but also on chitinases as a therapeutic target. Studies on chitinase inhibitors showed that methylxanthines (theophylline, caffeine, and pentoxifylline) are not only competitive inhibitors of fungal family 18 chitinases but are also active against human chitinases.Citation120 It was also reported that another chitinase inhibitor – demethylallosamidin – reduces hyperresponsiveness (induced by IL-13) in OVA-induced asthma model and may have potential as a therapeutic agent for asthma.Citation121 The results of recent studies that evaluated the efficacy of AMCase and CHIT1 inhibitors in animal models of asthma seem to be promising.Citation66,Citation122 A better understanding of the physiological activity of chitinases and chitinase-like proteins could accelerate the future development of chitinase-specific therapeutic agents in obstructive lung diseases.

Table 1 Selected Information About Main Chitinases and Their Role in Humans

Disclosure

KG reports fees for lectures and travel expenses from Boehringer Ingelheim, Chiesi, AstraZeneca, Polpharma and Roche, outside the submitted work. RK reports personal fees and non-financial support from Boehringer Ingelheim, Chiesi, and AstraZeneca; and personal fees from Polpharma, outside the submitted work. The authors report no other conflicts of interest in this work.

Acknowledgments

The authors thank Dr Marta Maskey-Warzechowska for her assistance in the preparation of the final version of the manuscript. The authors alone are responsible for the content and writing of the paper.

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