Bibliometric and Visualization Analysis of Stem Cell Therapy for Erectile Dysfunction

Abstract

Purpose

As a common male disease, erectile dysfunction (ED) seriously affects the physical and mental health of patients. In recent years, studies have continued to point out the great potential of stem cell therapy (SCT) in the treatment of ED. The purpose of this study is to comprehensively analyze the research of SCT for ED and understand the development trends and research frontiers in this field.

Methods

Publications regarding SCT and ED were retrieved and collected from the Web of Science Core Collection. CiteSpace and VOSviewer software were then utilized for bibliometric and visualization analysis.

Results

A total of 524 publications were eventually included in this study. The annual number of publications in this field was increasing year by year. China and the USA were the two most productive countries. Lin GT, Lue TF and Lin CS, and the University of California San Francisco where they worked were the most productive research group and institution, respectively. The journal with the largest number of publications was The Journal of Sexual Medicine, and the following were mostly professional journals of urology and andrology. Diabetes mellitus-induced ED and cavernous nerve injury-related ED were the two most commonly constructed models of ED in studies. Concerning the types of stem cells, mesenchymal stem cells derived from adipose and bone marrow were most frequently used. Moreover, future research would mainly focus on exosomes, tissue engineering technology, extracorporeal shockwave therapy, and clinical translation.

Conclusion

The research of SCT for ED will receive increasing global attention in the future. Our study provided bibliometric and visualization analysis of published literature, helping researchers understand the global landscape and frontiers in this field. More preclinical and clinical studies should be conducted to more deeply explore the underlying mechanisms of treatment and promote clinical translation.

Keywords:

• erectile dysfunction
• stem cells
• bibliometric analysis
• visualization
• VOSviewer
• citespace

Introduction

Erectile dysfunction (ED) is considered a condition wherein the penis is insufficiently rigid to achieve satisfactory sexual intercourse. Its prevalence among middle-aged and elderly men over 40 years old is as high as 52% and increases with age.^1,2 The number of patients suffering from ED is estimated to reach 322 million in 2025.³ Moreover, its impact on male patients is multifaceted. On the one hand, it affects the quality of sexual life and damages the relationship between couples.⁴ On the other hand, the penis is the continuation of the blood vessels of the whole body, and ED could serve as a predictor of cardiovascular diseases.⁵ Timely and effective diagnosis and treatment of ED could assist in enhancing the quality of life in patients and promote physical and mental health.

According to the cause, ED could be divided into three categories, namely the psychogenic, organic, and mixed types.⁶ Common clinical risk factors include diabetes mellitus (DM), smoking, high blood pressure, radical pelvic surgery, stroke, drugs, aging, and other causes, all of which could promote the occurrence of ED.^4,7 Phosphodiesterase type 5 inhibitor (PDE5i) is the most important drug in the current treatment of ED,⁸ with an effectiveness as high as 83%. However, patients with certain ED subtypes respond poorly to PDE5i, with response rates of 63% in DM-induced ED (DMED) and 48% in cavernous nerve injury (CNI)-related ED (CNI-ED).⁹ Second and third-line treatments encompass vacuum erection devices, intraurethral suppositories, intracavernous injections, and penile implants.⁴ However, limitations associated with these strategies, such as the inability to correct the pathological state of the penis and their restricted application due to side effects, cost, and unsatisfactory results,¹⁰ which highlight the urgent need for a comprehensive exploration of the pathological mechanism and treatment strategies for ED.

As is well documented, stem cells have the capability of self-renewal and can differentiate into mature cells. According to their differentiation potential, stem cells can be classified as totipotent (eg zygotes), pluripotent (eg embryonic stem cells), multipotent (eg mesenchymal stem cells), oligopotent, and unipotent cells.^11,12 The most frequently used and easily obtained stem cells are mesenchymal stem cells, which exert almost no tumorigenic effects and are not subject to ethical restrictions.¹³ Since Ernst Haeckel proposed the concept of stem cells in 1868, the exploration of stem cells and their application in diseases have been the focus of scientific research and clinical practice.¹⁴ Indeed, they hold significant implications in the treatment of various diseases and have considerably promoted the development of regenerative medicine.¹⁵ The application of stem cells in ED started later, with no corresponding reports published until the 21st century.¹⁶ However, stem cell therapy (SCT) in ED has achieved surprising phased objectives. A number of basic and clinical trials have established that SCT could partially restore erectile function caused by various factors and improve the quality of sexual life.^11,17,18 More importantly, stem cells can alleviate not only symptoms but also ED by addressing pathological mechanisms, including differentiating into specific effector cells and secreting numerous growth factors and exosomes, thereby improving the local pathological environment and stimulating tissue repair.^16,19,20

While many studies have contributed to the basic research and clinical translation of SCT in ED treatment, researchers are typically unable to quickly grasp current research progress, frontiers, and future development directions in this field based on the complicated literature. Traditional literature reviews are generally associated with a certain degree of subjectivity and one-sidedness, and cannot comprehensively and systematically elucidate the above questions. As a method of document analysis, bibliometrics allows for the aggregation of current research data in a certain field and its quantitative and qualitative analysis.²¹ CiteSpace and VOSviewer are commonly used analysis software, generating knowledge networks to facilitate researchers in understanding the past, present, and future of this field more conveniently and efficiently.²² Therefore, this study aimed to conduct bibliometric and visualization analysis of research on SCT for ED to provide a theoretical reference for future exploration in this field.

Materials and Methods

Data Sources and Search Strategy

In this study, the Web of Science Core Collection (WoSCC) database was searched for relevant articles. The search formula was set as follows: TS = (“erectile dysfunction” OR “impotence”) AND TS = (“stem cell*” OR “progenitor cell*”). The search was performed on 2023.8.15, and all documents were retrieved and collected within this day to mitigate the risk of time-related bias. The date of publication was limited to 2022 and before, and only publications in English were considered. This search only included original articles and reviews and excluded meeting abstracts, editorial materials, corrections, letters, processing papers, and early access articles. After screening the titles and abstracts, publications not related to the study topic were also excluded. Two researchers searched the database and screened relevant publications. Disagreements were arbitrated by the third researcher until a consensus was reached. Following these criteria, a total of 524 publications were eventually included in this study (Figure 1).

Figure 1 Flow diagram of literature selection.

Complete records and references were exported, including the title, author, journal, country/region, institution, abstract, keywords, references, etc. Given that our study was a bibliometric study and did not involve human or animal participants, the review and approval from the ethics committee were waived.

Data Analysis

In this study, GraphPad Prism 8.0 was used to analyze the publication information of the literature and the source of stem cells. VOSviewer 1.6.16 was utilized to examine the cooperative relationship between institutions and countries. Additionally, Scimago Graphica 1.0.36 was also employed for the country analysis.^23,24 Lastly, authors, journals, references, and keywords were explored using CiteSpace 6.2.R4.^25,26

Results

The Trend of Publications and Citations

As illustrated in Figure 2, the annual research on SCT for ED generally showed a fluctuating upward trend, indicating that this field has garnered increasing attention from the academic community. The initial study on SCT and ED in WoSCC was published in 2004,²⁷ with the first peak of publication in this field being in 2012. The number of publications after 2017 was basically the same as that in 2012 or even catching up. Documents published from 2007 to 2016 had a relatively high level of citations, with the most cited documents published in 2012. The aforementioned trend in citation levels may be affected by the year of publication; that is, earlier documents were more likely to be cited frequently.

Figure 2 Global trends in the number of articles and cited times.

Analysis of Countries/Regions and Institutions

In this study, the countries or regions of the authors were ranked by the number of publications related to SCT and ED (Table 1). China (192) and the USA (187) ranked first and second in terms of total number of published articles, respectively, accounting for over 70% of publications. Likewise, the top 2 countries in the H-index were also the USA (45) and China (38). The above results showed that these two countries had enormous advantages in this field and had published many high-quality documents. As for the average citations per item (ACI), the top 3 countries were Canada (64.9), Egypt (59.5), and Belgium (40.5), indicating that these three countries had been involved in research in this field in the early stage. To clearly reflect cooperative relationships, the distribution of countries or regions was re-examined after combining geographical location information (Figure 3A). In general, cooperation between countries globally was close. The USA had the highest intensity of cooperation with other countries, followed by China. The closest cooperation was also between China and the USA. In addition, Europe was currently the research center in this field and had the most frequent cooperation.

Table 1 The Top 10 Most Productive Countries/Regions on the Research of SCT for ED

Rank	Country/Region	Publications	Percentage, %	Citations	ACI	H-Index
1	China	192	36.6	4236	22.1	38
2	USA	187	35.7	6616	35.4	45
3	Italy	65	12.4	1415	21.8	23
4	South Korea	51	9.7	1006	19.7	19
5	Turkey	23	4.4	401	17.4	12
6	Belgium	21	4.0	850	40.5	16
7	England	21	4.0	691	32.9	13
8	Canada	17	3.2	1104	64.9	11
9	Japan	14	2.7	408	29.1	9
10	Egypt	12	2.3	714	59.5	6

Abbreviations: SCT, stem cell therapy; ED, erectile dysfunction; ACI, average citations per item.

Figure 3 Visualization results of countries/regions and institutions on the research of SCT for ED. (A) In national/regional cooperation, the size of the nodes represents the number of articles, and the depth of the color represents the intensity of collaboration. (B) In institutional cooperation, the node size represents the number of articles. Blue represents the early stage of research and yellow represents the recent stage of research.

Given that authors and institutions require funds to conduct scientific research, the investment of funds is related to the country’s leading level in this field. As summarized in Table 2, the National Natural Science Foundation of China funded the largest number of publications. Eight of the top 10 funds were from China and the USA, which was consistent with the research strengths of these two countries in this field.

Table 2 The Top 10 Funds on the Research of SCT for ED

Rank	Foud	Publications	Percentage, %
1	National Natural Science Foundation Of China	83	15.8
2	National Institutes Of Health	58	11.1
3	United States Department Of Health Human Services	58	11.1
4	National Research Foundation Of Korea	20	3.8
5	National Institute Of Diabetes Digestive Kidney Diseases	18	3.4
6	National Natural Science Foundation Of Guangdong Province	17	3.2
7	Department Of Veterans Affairs	9	1.7
8	China Postdoctoral Science Foundation	6	1.1
9	Ministry Of Education Culture Sports Science And Technology Japan Mext	6	1.1
10	China Scholarship Council	5	1.0

Abbreviations: SCT, stem cell therapy; ED, erectile dysfunction.

The top 10 institutions in terms of number of publications related to SCT and ED are detailed in Table 3. The University of California San Francisco (42) in the USA ranked first, and its ACI (48.1) and H-index (25) were also among the highest. It was followed by several Chinese institutions followed, such as Sun Yat-sen University (26), Shanghai Jiao Tong University (24), and Peking University (21). It is worthwhile mentioning that while Nanjing University did not publish many articles (14), it ranked first in ACI (50.3), signifying that the documents produced by this institution were published earlier or of higher quality. Furthermore, a visual analysis of the institutions was implemented to generate a collaborative network (Figure 3B). The University of California San Francisco remained at the center of the network. Several institutions in China appeared to have initiated their efforts relatively late, indicating that China has made tremendous progress in recent years to achieve its current advantage in this field.

Table 3 The Top 10 Most Productive Institutions on the Research of SCT for ED

Rank	Institution	Country/Region	Publications	Percentage, %	Citations	ACI	H-index
1	University of California San Francisco	USA	42	8.0	2019	48.1	25
2	Sun Yat-sen University	China	26	5.0	557	21.4	10
3	Shanghai Jiao Tong University	China	24	4.6	376	15.7	10
4	Peking University	China	21	4.0	717	34.1	15
5	Catholic University of Korea	South Korea	19	3.6	370	19.5	11
6	University of Catania	Italy	18	3.4	250	13.9	13
7	Huazhong University of Science and Technology	China	17	3.2	204	12	9
8	Tulane University	USA	17	3.2	470	27.6	11
9	Nanjing University	China	14	2.7	704	50.3	12
10	University of California Los Angeles	USA	13	2.5	367	28.2	9

Abbreviations: SCT, stem cell therapy; ED, erectile dysfunction; ACI, average citations per item.

Analysis of Authors

The top 10 most productive authors were ranked according to the number of publications in the field of SCT and ED (Table 4). Lin GT (35), Lue TF (33), and Lin CS (18) were the top 3 authors. These 3 authors were all from the University of California San Francisco, and their ACI and H-index were both at the leading level. Among the 10 authors, 6 were from the USA, 2 were from South Korea, and the remaining two were from Belgium and Italy. No Chinese author ranked among the top 10. This may be ascribed to the fragmented nature of Chinese research and the lack of authors’ influence.

Table 4 The Top 10 Most Productive Authors on the Research of SCT for ED

Rank	Author	Country/Region	Publications	Percentage, %	Citations	ACI	H-Index
1	Lin GT	USA	35	6.7	1642	46.9	20
2	Lue TF	USA	33	6.3	1762	53.4	25
3	Lin CS	USA	18	3.4	1263	70.2	18
4	Albersen M	Belgium	17	3.2	776	45.6	15
5	Kim SW	South Korea	14	2.7	301	21.5	10
6	La Vignera S	Italy	13	2.5	216	16.6	12
7	Zhang YY	USA	12	2.3	424	35.3	9
8	Lee JY	South Korea	12	2.3	310	25.8	9
9	Bivalacqua TJ	USA	11	2.1	360	32.7	13
10	Hellstrom WJG	USA	11	2.1	310	28.2	10

Abbreviations: SCT, stem cell therapy; ED, erectile dysfunction; ACI, average citations per item.

The visual network and clustering analysis of authors’ cooperation were performed to reflect collaborations and research directions among authors (Figure 4). Academic groups were formed among American authors, primarily focusing on the research of adipose-derived mesenchymal stem cells (ADSCs). Several authors from China worked closely with academic groups in the USA, such as Liu Jihong and Xin Zhongcheng. Academic groups were also observed among Korean authors, mainly focusing on research on streptozotocin-induced diabetic rats.

Figure 4 Visualization results of authors on the research of SCT for ED. (A) Visual network analysis of authors’ cooperation. The size of the nodes represents the number of articles, and the depth of the color represents the average publication year of the articles. (B) Clustering analysis of authors’ cooperation. The same color represents a cluster.

Analysis of Journals

Table 5 lists the top 10 journals by number of publications related to SCT and ED. The Journal of Sexual Medicine ranked first in terms of number of publications (62), ACI (38.2), and H-index (28), indicating that this is a popular journal for research in this field. It was followed by the International Journal of Impotence Research (28), Andrology (20), Asian Journal of Andrology (18), and Translational Andrology and Urology (14), which are all professional journals related to urology and andrology. Moreover, the 2022 impact factors (IF) of these journals were all slightly low, ranging from 2.5 to 5.5, which may be limited by the average IF level of professional journals in the field of andrology.

Table 5 The Top 10 Journals Publishing Research on SCT and ED

Rank	Journal	Publications	Percentage, %	Citations	ACI	H-Index	IF (2022)
1	The Journal of Sexual Medicine	62	11.8	2367	38.2	28	3.5
2	International Journal of Impotence Research	28	5.3	619	22.1	14	2.6
3	Andrology*	20	3.8	295	14.8	9	4.5
4	Asian Journal of Andrology	18	3.4	347	19.3	12	2.9
5	Translational Andrology and Urology	14	2.7	167	11.9	6	2.0
6	BJU International	13	2.5	401	30.8	10	4.5
7	PLOS ONE	11	2.1	333	30.3	7	3.7
8	International Journal of Molecular Science	11	2.1	167	15.2	5	5.6
9	Andrologia	10	1.9	139	13.9	4	2.4
10	Sexual Medicine Reviews	10	1.9	182	18.2	7	3.6

Notes: *In this study, the number of publications in International Journal of Andrology and Journal of Andrology were calculated separately and not combined with Andrology.

Abbreviations: SCT, stem cell therapy; ED, erectile dysfunction; ACI, average citations per item.

The dual-map overlay of journals was plotted to visualize citation relationships between journals (Figure 5).²⁸ The left portion of the map represents citing journals, while the right portion represents cited journals. The two most visible paths on the map (orange and green) represent the most important citation routes in this area. Studies published in Molecular, Biology, Genetics, Health, Nursing, and Medicine-related journals tended to be cited in Molecular, Biology, Immunology, Medicine, Medical, and Clinical-related journals.

Figure 5 The dual-map overlay of journals on the research of SCT for ED. The citing journals are on the left and the cited journals are on the right. The colored connections represent the citation relationship.

Analysis of References

Highly cited references in the field were visualized and are presented in Figure 6A. The publication with the highest citation intensity was published in 2010, titled “Injections of adipose tissue-derived stem cells and stem cell lysate improve recovery of erectile function in a rat model of cavernous nerve injury”²⁹ This study principally demonstrated the therapeutic effect of ADSCs and cell lysate on erectile function in rats with CNI. Clustering the above highly cited references yielded a total of 6 clusters, namely smooth muscle, ADSCs, ED, coronary artery disease, neurogenic impotence, and endothelial dysfunction (Figure 6B). Most of the highly cited documents were related to research on ADSCs.

Figure 6 Visualization results of references on the research of SCT for ED. (A) Co-citation analysis of references. The size of the nodes represents the number of citations of the references, and the depth of the color represents the publication year of the references. (B) Clustering analysis of references. The same color represents a cluster. (C) Burst analysis of references. The red portion of the blue line represents the burst duration of the reference.

Frequent citations of certain documents over a period of time indicated new breakthroughs or hot spots in this field. In other words, the top 20 references with the strongest citation bursts deserve attention (Figure 6C). The strongest one was “Mesenchymal stem cells alone or ex vivo gene modified with endothelial nitric oxide synthase reverse age-associated erectile dysfunction”, published by Bivalacqua TJ et al in 2007 (strength = 16.43; from 2008 to 2012).³⁰ In this article, the authors pointed out that genetically modified mesenchymal stem cells could differentiate into endothelial cells (ECs) and smooth muscle cells (SMCs) and ultimately improve erectile function in aged rats. References ranking second and third (strength = 16.36 and 15.97; both from 2017 to 2022) in burst strength were both clinical trials of stem cells used in patients with ED following radical prostatectomy.^31,32 The effectiveness and safety of SCT in clinical patients with ED were initially validated. The remaining 4 references whose citation burst period ended in 2022 were 2 clinical trials regarding SCT for the treatment of ED,^33,34 1 guideline,³⁵ and 1 review.³⁶ The above results indicated that the clinical application of stem cells seemed to have received more attention in recent years. The remaining references listed in Figure 6 are detailed in the References List.^37–48

Analysis of Keywords

Given that keywords represent the critical information in the article, the co-occurrence analysis of keywords was conducted and is presented in Figure 7A. The five keywords with the highest frequency of occurrence were ED (343), rat model (116), radical prostatectomy (83), stem cells (74), and CNI (64), implying that rats were the most commonly used experimental animals in basic research on ED, with CNI being one of the major causes of ED. The results of clustering analysis exposed that high-frequency keywords were summarized into 6 clusters, namely endothelial dysfunction, DM, CNI, endothelial growth factors, ED, and smooth muscle (Figure 7B). Combined with temporal information, a timeline visualization of keywords was performed (Figure 7C). DM and CNI appeared throughout, indicating that these two causes had been the research focus in this area.^49,50 Research on ECs and SMCs was ongoing until 2022, signifying that normalization of these two components in the corpus cavernosum was critical to erection.^19,20,41

Figure 7 Visualization results of keywords on the research of SCT for ED. (A) Co-occurrence analysis of keywords. The size of the nodes represents the number of occurrences, and the depth of the color represents the average occurrence year of the keywords. (B) Clustering analysis of keywords. The same color represents a cluster. (C) The timeline visualization and clusters of keywords. (D) Burst analysis of references. The red portion of the blue line represents the burst duration of the keyword.

The top 20 keywords with the strongest citation bursts are depicted in Figure 7D. Nitric oxide synthase (NOS) was the keyword with the earliest appearance, highest burst strength, and most extended duration. This enzyme has the ability to catalyze the substrate to generate nitric oxide (NO), which directly initiates and maintains penile erection.⁵¹ The citation bursts for intracavernous injection, extracorporeal shockwave therapy, SCT, and delivery continued until 2022, suggesting that these keywords are currently focal points. Intracavernous injection remains the most important approach to introducing stem cells into recipients to exert therapeutic effects. Meanwhile, extracorporeal shockwave therapy not only activates local stem cells/progenitor cells in the penis but also improves the efficacy of SCT when used in conjunction with stem cells.^52,53

Analysis of Stem Cell Sources

Considering that stem cells in SCT may originate from different species or tissues, original articles were manually searched for information on stem cell sources. A total of 164 basic experimental articles on exogenous stem cells were included, 10 of which employed stem cells derived from two tissues, so the final count was 174 articles. Regarding the sources of different species, rat-derived stem cells accounted for 62.1% of all stem cells (108), followed by human-derived stem cells accounting for 30.5% (53) (Figure 8A). Concerning the sources of different tissues, adipose (48.3%, 84) ranked first, followed by bone marrow (27.0%, 47) and umbilical cord (8.0%, 14) (Figure 8B). Based on these observations, a comprehensive analysis regarding the sources of stem cells was performed (Figure 8C). Attributed to the fact that rats were the most frequently used experimental animals for exploring ED, rat adipose (66) and rat bone marrow (33) were the top two sources of stem cells. Human adipose (16), human umbilical cord (13), human bone marrow (12), and other sources were lower down the list. In short, whether of rat or human origin, adipose and bone marrow remained the most common tissue sources of stem cells in SCT. In addition, some applications of other sources, such as muscle and urine, were also made.^54,55

Figure 8 Source analysis of stem cells. (A) Pie chart of stem cells derived from different species. (B) Pie chart of stem cells derived from different tissues. (C) Histogram of stem cells from different sources.

Discussion

In recent years, research results on SCT have accumulated, and SCT has been found to exert therapeutic effects on various diseases, including diabetes, neurological disorders, cardiovascular disorders, and cancer. In addition, SCT plays a pivotal role in regenerative medicine.^12,56 In view of the limitations of traditional treatment methods for ED and the underlying effects of SCT, the latter has progressively become the focus of the ED treatment field and has attracted the attention of an increasing number of researchers. In this study, CiteSpace and VOSviewer were utilized to conduct bibliometric and visualization analysis of research in the field of SCT and ED, aiming to offer valuable insights into the development process, research trends, and research hotspots in this field. Based on the analysis of 524 original studies and reviews, our results showed that the literature involving SCT and ED increased yearly. The above information revealed that SCT is a candidate treatment for ED and is likely to be a key research direction in regenerative medicine for ED in the future.

General Information

China (first) and the USA (second) were the two countries that conducted the highest number of studies on SCT and ED, and their investment in scientific research was also enormous. The strength of China may be slightly inferior, as reflected in its late start, marginally lower ACI and H-index, and the lack of authoritative scholars and high-impact literature. In addition, there were extensive connections and cooperation between countries, institutions, and scholars worldwide, especially between China and the USA. The research group led by Lue TF, Lin GT, and Lin CS from the University of California San Francisco excelled in this field. They have published a large number of studies, many of which were high-impact documents, and have made significant contributions to the development of the field.^38,42,43 Publications on the application of SCT in ED tended to be published in specialized journals of urology and andrology, such as The Journal of Sexual Medicine, Andrology, and Asian Journal of Andrology. Moreover, an increasing number of high-quality documents have been published in journals in other fields and comprehensive journals in recent years,^49,57,58 indicating that the scientific research community is widely recognizing research in this field.

Mechanism of SCT for ED

Following sexual stimulation, NOS in nerve cells and ECs is activated and generates NO, which diffuses into adjacent SMCs and reduces intracellular Ca²⁺ concentration, ultimately leading to vasodilation and penile erection.⁵¹ The damage to any of the above links may lead to the occurrence of ED. Stem cells possess differentiation potential and could differentiate into nerve cells, ECs, and SMCs after transplantation, thereby replacing damaged tissue and preserving homeostatic function. A large number of bioactive factors are also released in a paracrine manner by implanted stem cells, thereby improving the local pathological environment and accelerating tissue repair.^41,59 However, more and more studies have documented that paracrine effects seem to be the primary mechanism of SCT compared with cellular differentiation effects.^10,18 Only a small number of stem cells could be detected after transplantation. It is difficult to distinguish whether the restoration of effector cells is due to the differentiation or paracrine activity of stem cells. These are the reasons for the controversy regarding the repair mechanism mentioned above. In addition, the advancement of research on exosomes in recent years uncovered that stem cell-secreted exosomes also play a fundamental role in penile tissue repair.⁶⁰ Compared with the injection of stem cells, exosomes have many benefits, such as higher stability, no risk of tumorigenesis, and lower immunogenicity.⁶¹ Exosomes secreted by stem cells seem to be a promising novel direction for the research of ED.

Improvement of SCT Efficacy

Compared to original stem cells, various methods have been explored to increase the efficacy of SCT for the treatment of ED. Genetically modifying stem cells to manufacture engineered stem cells has been established to enhance cellular function. Stem cells overexpressing a series of growth factors all show beneficial effects on the recovery of erectile function.^45,62,63 The expression of certain microRNAs also affects the activity of stem cells; thus, regulating the levels of specific microRNAs in stem cells is another viable strategy.^64,65 The enhancement of cellular retention, cellular differentiation, and cellular paracrine effects after transplantation are the mechanisms by which the abovementioned engineered stem cells exert powerful effects.⁶⁶ The research and application of novel biomaterials have brought new opportunities for the development of SCT in ED. The retention time of stem cells in the corpus cavernosum is extended when combined with materials such as hydrogels, scaffolds, and magnetic nanoparticles.^58,67,68 In addition, the combination of stem cells and existing therapies, such as shockwave therapy and certain drugs, could also be effective.^52,69 Comprehensive management of the above methods often exerts a powerful therapeutic effect.

Clinical Trials

Although current research on SCT and ED is chiefly centered on animal experiments, there are still some clinical trials exploring the effectiveness and safety of SCT in the treatment of ED.¹⁷ Among the 15 retrieved clinical trials (Supplementary Table 1), the types of stem cells used in the studies were diverse, with the majority of patients developing DMED and CNI-ED. All studies showed that patients experienced no severe adverse events or were not at increased risk of severe adverse events during SCT. In these clinical studies, SCT exerted an excellent therapeutic effect on ED. Erectile function was improved in almost all patients except those suffering from incontinence with severe damage to the cavernous nerve. The small study size and short follow-up period are weaknesses of the current studies. High-quality, large-scale, and long-term clinical studies are still needed to further evaluate the feasibility of promoting SCT to clinical patients with ED.

Unresolved Problems

Although the therapeutic effect of SCT on ED has been established, some issues remain unclear. Owing to the limited follow-up time of SCT (2–4 months for animals and up to 12 months for patients), the long-term effects and tumorigenic potential of stem cells after transplantation remain elusive. Intracavernous injection is the predominant route of administration, but several other methods exist, including intravenous injection, injured cavernous nerve cover, and periprostatic implantation. Each administration method has its own advantages and disadvantages. Hence, it is challenging to determine the ideal route of administration. Furthermore, the optimal timing of injection, the number of cells to be injected, the frequency of injection, and the use of autologous or allogeneic cells are yet to be clarified and need to be determined by further research.¹³

Limitations

Inevitably, there are some limitations in this study. To begin, relevant articles were solely screened in the WoSCC database, the most widely used database globally. Other databases, such as Scopus and Embase, were not included in the search. Next, our study exclusively focused on original studies and reviews. Other types of publications were excluded, which may lead to omissions. Finally, due to the low number of citations in some high-quality documents published in recent years, these documents may be drowned in the vast sea of literature data.

Conclusion

Based on bibliometric and visualization analysis of the research related to SCT and ED, the present study scientifically, comprehensively, and systematically analyzed the research status and frontiers in this field. The research of SCT in ED is booming and receiving increasing attention. The collaboration between different countries, institutions and authors around the world was extensive and drove this field forward together. Our research provided a theoretical reference and guidance for preclinical and clinical research on the use of SCT for the treatment of ED.

Abbreviations

ED, erectile dysfunction; SCT, stem cell therapy; DM, diabetes mellitus; PDE5i, phosphodiesterase type 5 inhibitor; DMED, diabetes mellitus-induced ED; CNI-ED, cavernous nerve injury-related ED; WoSCC, the Web of Science Core Collection; ACI, the average citations per item; ADSCs, adipose-derived mesenchymal stem cells; ECs, endothelial cells; SMCs, smooth muscle cells; NOS, nitric oxide synthase; NO, nitric oxide.

Author Contributions

All authors made a significant contribution to the work reported, whether that is in the conception, study design, execution, acquisition of data, analysis and interpretation, or in all these areas; took part in drafting, revising or critically reviewing the article; gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work.

Disclosure

The authors report no conflicts of interest in this work.

Acknowledgments

We would like to thank all authors who participated in the study of SCT and ED, and Home for Researchers editorial team (http://www.home-for-researchers.com) for language editing service.

Data Sharing Statement

All data analyzed were included in this paper; further requests can be consulted and data can be obtained from the correspondent author.

Additional information

Funding

This work was supported by the National Natural Science Foundation of China (No. 82201775) and the Joint Construction Project between Medical Science and Technology Research Project of Henan Province (No. LHGJ20220343).