Anemia among adult HIV patients in Ethiopia: a hospital-based cross-sectional study

Abstract

Background

Anemia is a major public health problem in HIV patients around the world. It has a negative effect on the quality of life of HIV patients and progression of the HIV disease. In the sub-Saharan African setting, including Ethiopia where both HIV infection and under-nutrition are expected to be high, there is a paucity of data on the matter. This study was aimed to reveal the magnitude and factors associated with anemia among adult HIV patients in Debre-Tabor Hospital, northwest Ethiopia.

Methods

A hospital-based cross-sectional study was used among adult HIV patients in Debre-Tabor Hospital from April 1 to May 30, 2015. The diagnosis of anemia was made following the 2011 World Health Organization recommendation on hemoglobin cut-off points. Univariable and multivariable logistic regression was carried out to assess factors associated with anemia.

Results

A total of 377 patients’ charts were reviewed. Most of the participants (n=237, 62.9%) were taking antiretroviral treatment (ART). The overall prevalence of anemia was 23% (95% CI: 19.1, 27.6). Being ART-naïve (adjusted odds ratio [AOR]: 3.37; 95% CI: 1.59, 7.14), having treatment history with anti-tuberculosis (TB) drug (AOR: 3.2; 95% CI: 1.19, 8.67), taking zidovudine (ZDV)-containing ART regimen (AOR: 2.14; 95% CI: 1.03, 4.57), and having recent CD4+ T-lymphocytes count of <200 cells/μL (AOR: 2.13; 95% CI: 1.04, 4.36) were associated with occurrence of anemia among adult HIV patients.

Conclusion and recommendation

Anemia continues to be a major co-morbidity among adult HIV patients in Ethiopia. Adult HIV patients who are taking ZDV-containing ART, with a history of TB treatment, have a low CD4+T-lymphocytes count and are ART-naïve should be carefully screened and treated for anemia.

Keywords:

• anemia
• HIV/AIDS
• antiretroviral treatment
• ZDV toxicity
• Ethiopia

Background

Anemia is a major health problem globally, which can cause a vicious impediment on quality of life, mortality, morbidity, and socioeconomic progress of a country. It affects more the developing than the developed countries.¹ Anemia is the most common hematologic abnormality among HIV-infected individuals; this includes those who are taking antiretroviral treatment (ART) and those who are ART-naïve.²

Anemia among HIV patients can lead to impaired physical functioning, psychological distress, poor quality of life, accelerated disease progression, and shorter life expectancy.³ Although anemia can occur at any stage of HIV infection, its severity is correlated with progression of the HIV disease stage.⁴ A study conducted in the very early years of HIV discovery had shown the overall prevalence of anemia to be about 28% among people with HIV infection in the pre-AIDS stage of the disease, whereas it could reach as high as 71% in the advanced or AIDS stage of the HIV disease.⁵

According to reports, the prevalence of anemia among adult HIV patients taking ART ranges between 23% and 50% globally and 24% and 58% in Africa.^6–9 Generally, the prevalence of anemia tends to be higher in ART-naïve patients compared to ART users. In Ethiopia, the prevalence of anemia among ART-naïve adult HIV patients was reported to reach up to 35%.¹⁰ A study done 3 years ago in the southwest part of Ethiopia has shown the prevalence of anemia to be 29.9% and 16.2% among ART-naïve and ART-experienced HIV patients, respectively.¹¹ Another study conducted 3 years ago in southern Ethiopia has shown the prevalence of anemia among ART-naïve adult HIV patients to be 23.4%, and the prevalence after 6-month course of ART was only 12%.¹²

Various reports have identified different factors to have association with anemia among adult HIV patients including gender, residence, marital status, educational status, income, duration of ART, type of ART regimen, history of anti-tuberculosis (TB) drug treatment, presence of opportunistic infections (OIs), advanced stage of the HIV disease, CD4+T-lymphocyte count <200 cells/μL, white blood cell (WBC) count <4,000 cells/μL, and platelets count <200,000 cells/μL.^7,11–19

However, the reported magnitudes and associated factors are different from one geographic area to another and have shown different time trends as the interplay between the various socioeconomic, health, and nutritional conditions alter in sub-Saharan country setting. Therefore, this study was aimed at assessing the prevalence of anemia and its associated factors among adult HIV patients who were getting follow-up HIV care at Debre-Tabor Hospital, northwest Ethiopia.

Methods

Study design and setting

Hospital-based quantitative cross-sectional study was conducted from April 1 to May 30, 2015, at Debre-Tabor Hospital located in northwest Ethiopia. The hospital is found in Amhara Regional State in the town called Debre-Tabor, which is 665 km northwest of the capital city Addis Ababa. The hospital has a catchment population of nearly 2.3 million people and is one of the HIV-care providing centers in the region. It has an average daily patient flow of 20–30 adult HIV patients in the working hours of the HIV-care clinic of the hospital excluding emergency presentations on duty hours.

Sample size determination, sampling procedure, and study subjects

The sample size was calculated based on single population proportion formula using a confidence interval (CI) of 95% and previous prevalence of anemia being 35% among ART-naïve HIV patients,¹⁰ $n=\frac{{\left(Z_{\frac{a}{2}}\right)}^{2}P(1-P)}{d^2}$

Where, n = sample size, P = prevalence of anemia among ART-naïve HIV patients and d = assumed marginal error.

Finally, a minimum sample size of 385 was calculated after anticipating a 10% non-response rate. Simple random sampling technique was used to recruit 385 patients’ charts from a total of 689. The study populations were all adult HIV patients above the age of 18 years who were on HIV follow-up care at Debre-Tabor Hospital during January 1, 2010 to December 30, 2014, regardless of their ART status. Those adult patients with incomplete information for hemoglobin (Hb) status on the chart, either at baseline or during follow-up; pregnant women; and women in the post-partum period were excluded from the study.

Data collection instrument and procedures

Pretested and structured questionnaires were used to collect the data. The questionnaires were constructed with sociodemographic and economic characteristics; hematological characteristics; and disease stage, medication treatment, and co-morbidity status-related characteristics. The questionnaires were prepared in English. A total of four health professionals, three ART nurses as data collectors, and one public health officer as supervisor were recruited for the data collection process of this study. The 2011 World Health Organization (WHO) report on Hb concentration level to diagnose anemia was used in the hospital.²⁰ Accordingly, anemia for males was defined as Hb concentration <13 g/dL (11.0–12.9 g/dL=mild; 8.0–10.9 g/dL moderate, and <8.0 g/dL=severe), whereas anemia for females was defined as Hb <12.0 (11.0–11.9 g/dL=mild, 8.0–10.9 g/dL=moderate, and <8.0 g/dL=severe).

Data quality control

The data collection tool was pre-tested without Hb status determination on 5% of sample size charts of HIV/AIDS patients before the actual data collection period. Training was given to recruited data collectors and supervisor. All questionnaires were checked during the data collection period by the responsible supervisor on daily basis to check completeness, clarity, and consistency. Before analysis, the data were cleaned thoroughly to check for errors during entry.

Data processing and analysis

Data were entered into Epidemiological Information (EPI INFO), version 7.1 and analyzed using the Statistical Package for Social Sciences (SPSS) version 20. Descriptive statistics, including frequencies and proportions, was used to summarize the study variables. Univariable and multivariable logistic regression was used for factors associated with Hb status of the study subjects. Those variables with a P-value of <0.2 in the univariable analysis were exported to multivariable analysis to control the possible effect of confounders. The adjusted odds ratio (AOR) at a 95% confidence interval and P-value of ≤0.05 was used to declare the statistical significance in the multivariable analysis.

Ethical approval

Ethical clearance was obtained from the institutional review board of the Institute of Public Health of University of Gondar. Permission letter for the next steps was secured from the Debre-Tabor Hospital administrative body. Written informed consent was obtained from participants, and the confidentiality of information obtained was maintained by coding and restricting access to the questionnaire.

Results

We did not include the planned sample of 385 patients’ charts due to incomplete documentations identified in some charts. Therefore, a total of 377 patients’ chart were reviewed in this study out of which 237 (62.9%) were from patients taking ART and 140 (37.1%) were ART-naïve. More than 60% of the study participants were female, and the mean age of the study subjects was 35.21 with a standard deviation (SD) of ±9.27 years. Three hundred and seventy-five (99.5%) of the participants were Amhara by ethnicity and 367 (97.3%) were orthodox Christian by religion. A total of 291 participants (77.2%) were urban residents and 191 (50.5%) were married (Table 1).

Table 1 Socioeconomic and demographic characteristics of adult HIV patients at Debre-Tabor Hospital, northwest Ethiopia, 2015 (n=377)

Background characteristics	Not anemic, n (%)	Anemic, n (%)
Sex
Male	110 (37.9)	33 (37.9)
Female	180 (62.1)	54 (62.1)
Age of the patient, years
15–29	71 (24.5)	21 (24.1)
30–39	126 (43.4)	44 (50.6)
40–49	71 (24.5)	16 (18.4)
≥50	22 (7.6)	6 (6.9)
Residence
Urban	226 (77.9)	65 (74.7)
Rural	64 (22.1)	22 (25.3)
Religion
Orthodox Christian	284 (97.9)	83 (95.4)
Othersa	6 (2.1)	4 (4.6)
Marital status of the patient
Single	54 (18.6)	22 (25.3)
Married	149 (51.4)	42 (48.3)
Divorced	31 (10.7)	6 (6.9)
Widowed	45 (15.5)	14 (16.1)
Separated	11 (3.8)	3 (3.4)
Ethnicity
Amhara	288 (99.3)	87 (100)
Tigrie	2 (0.7)	0 (0.0)
Educational status
Unable to read and write	94 (32.4)	17 (19.5)
Read and write	19 (6.6)	5 (5.7)
Primary school (years 1–8)	65 (22.4)	27 (31.0)
Secondary school (years 9–12)	59 (20.3)	21 (24.1)
College/university	53 (18.3)	17 (19.5)
Employment status of the patient
Civil servant	74 (25.5)	26 (29.9)
Daily laborer	34 (11.7)	7 (8.0)
Merchant	41 (14.1)	14 (16.1)
House wife	71 (24.5)	19 (21.8)
Farmer	31 (10.7)	11 (12.6)
Student	17 (5.9)	2 (2.3)
Unemployed	9 (3.1)	1 (1.1)
Otherb	13 (4.5)	7 (8.0)

Notes:

a Muslims and protestants,

b sex workers, carpenters, shop keepers, waiters, retired workers, and garage workers.

Eighty-seven participants (23%) were found to have anemia out of whom 61 were among the 112 ART-naïve patients making the prevalence of anemia in this group 54.5%. One hundred twenty-six (33.4%) participants had a CD4+ T-lymphocyte count <200 cells/μL. Seventy-three (19.4%) had a WBC count <4000 cells/μL. Seventy-six (20.2%) study participants had a platelet count of <200,000 cells/μL. Nearly three fourths of patients taking ART were found to have a normal nutritional status (Table 2).

Table 2 Clinical characteristics of adult HIV patients at Debre–Tabor Hospital, northwest Ethiopia, 2015 (n=377)

Background characteristics	Not anemic, n (%)	Anemic, n (%)
CD4 count (cells/mm^3)
<200	68 (23.4)	58 (66.7)
≥200	222 (76.6)	29 (33.3)
WBC count (cells/mm^3)
<4000	36 (12.4)	37 (42.5)
≥4000	254 (87.6)	50 (57.5)
Platelet count (cells/mm^3)
<200,000	42 (14.5)	34 (39.1)
≥200,000	248 (85.5)	53 (60.9)
Nutritional status (Body mass index)
Normal	222 (76.6)	59 (67.8)
Under weight	56 (19.3)	26 (29.9)
Over weight	11 (3.8)	2 (2.3)
Obesity	1 (0.3)	0 (0.0)
WHO clinical stage
Stage I	169 (58.3)	18 (20.7)
Stage II	63 (21.7)	5 (5.7)
Stage III	50 (17.2)	47 (54.0)
Stage IV	8 (2.8)	17 (19.5)
ART exposure status
Naïve	79 (27.2)	61 (70.1)
Users	211 (72.8)	26 (29.9)

Note: Body mass index classification: normal: 18.5–24.9 kg/m²; under weight: <18.5 kg/m²; overweight: 25.0–29.9 kg/m²; obesity ≥30 kg/m².

Abbreviations: ART, antiretroviral therapy; WBC, white blood cell; WHO, World Health Organization.

Hundred and ninety-one (50.7%) of the participants have had OIs, and <7% of the participants were found to be at the fourth stage of WHO’s clinical disease classification. One hundred and twelve (29.7%) of the study participants were taking zidovudine (ZDV) containing ART regimen. More than 90% of the study participants had no history of anti-TB treatment (Table 3).

Table 3 Factors associated with anemia among adult HIV patients at Debre–Tabor Hospital, northwest Ethiopia, 2015 (n=377)

Characteristics	Anemia status
	Yes, n (%)	No, n (%)	COR (95% CI)	AOR (95% CI)
Educational status
Unable to read and write	17 (15.3)	94 (84.7)	1	1
Read and write	5 (20.8)	19 (79.1)	1.46 (0.48, 4.43)	0.71 (1.78, 2.82)
Primary school (years 1–8)	27(29.3)	65 (70.7)	2.29 (1.16, 4.55)	1.61 (0.65, 4.00)
Secondary school (years 9–12)	21 (26.3)	59 (73.7)	1.97 (0.96, 4.03)	1.51 (0.58, 3.92)
College/university	17 (24.3)	53 (75.7)	1.77 (0.84, 3.76)	1.56 (0.58, 4.18)
ART regimen
ZDV-containing	46 (41.1)	66 (58.9)	3.80 (2.30, 6.29)	2.14 (1.03, 4.57)
Not ZDV-containing	41 (15.5)	224 (84.5)	1	1
Anti-TB treatment history
Yes	22 (62.9)	13 (37.1)	7.21 (3.45, 15.07)	3.23 (1.20, 8.69)
No	65 (19)	277 (81)	1	1
ART exposure status
Naïve	61 (43.6)	79 (56.4)	6.27 (3.70, 10.61)	3.24 (1.55, 6.80)
Users	26 (11)	211 (89)	1	1
CD4+ T-lymphocytes (Cells/mL)
<200	58 (46)	68 (54)	6.53 (3.87, 11.00)	2.06 (1.01, 4.19)
≥200	29 (11.6)	222 (88.4)	1	1

Abbreviations: AOR, adjusted odds ratio; ART, antiretroviral therapy; COR, crude odds ratio; TB, tuberculosis; ZDV, zidovudine.

In the bivariate logistic regression analysis, educational status, types of ART regimen, history of treatment with anti-TB drugs, duration of ART usage, history of OI, WHO ; ZDV, Zidovudine. stage of HIV disease, CD4+ T-lymphocyte count, WBC count and platelets count were significantly associated with anemia. In the multivariate binary logistic regression analysis, type of ART regimen, ART-usage status, history of treatment with anti-TB drugs, and CD4+ T-lymphocyte count remained to be significantly associated with anemia (Table 3).

Being ART-naïve was found to be associated with anemia (AOR: 3.37; 95% CI: 1.59, 7.14). Having treatment history with anti-TB drug was also found to be associated with anemia (AOR: 3.2; 95% CI: 1.19, 8.67). Taking ZDV-containing regimen was found to be associated with anemia (AOR: 2.1; 95% CI: 1.03, 4.57). Having a recent CD4+ T-lymphocyte count of <200 cells/μL was also found to be associated with anemia (AOR: 2.1; 95% CI: 1.04, 4.36).

Discussion

Our study has demonstrated a high overall prevalence (23%) of anemia among HIV patients who are ART-naïve and taking ART. This finding is concordant with a study done 3 years ago in the southwest Ethiopia (23.1%);¹³ similar findings were reported from studies in India (23%)⁶ and Ghana (24%).⁸ The prevalence was lower compared to the a study done 3 years ago from northwest Ethiopia (35%); however, that study included only ART-naïve HIV patients.¹⁰ Another study also done 3 years ago in southern Ethiopia reported the prevalence of anemia to be 23.4% among ART-naïve adult HIV patients and only 12% after 6-month course of ART.¹² Studies in Indonesia⁷ (49.6%), Nigeria⁹ (57.5%), and Uganda¹⁸ (47.8%) have reported a much higher prevalence of anemia compared to our study. However, in addition to the sociodemographic differences, those reports reflected the prevalence of anemia among ART-naïve patients. In our study where two thirds of the participants were taking ART, it is expected to get lower prevalence.

Various studies have pointed out multiple potential factors associated with anemia among HIV patients.^7,11–19 Being ART-naïve was found to be associated with anemia in our study. This finding is in agreement with the reports from southern Ethiopia,^12,13 Ghana,⁸ and South Africa.¹¹ This can be explained by the various direct and indirect effects of the effective ART. One plausible explanation is the fact that ART can suppress HIV, a virus which is known to directly affect the bone marrow; therefore, by suppressing the viral load ART could prevent anemia. The other explanation could be related to the indirect effect of ART, which is expected to improve the immunity of HIV patients thereby decreasing the occurrence of multiple OIs, which are identified to potentially cause anemia.

Type of ART regimen, ZDV-containing regimen, was also found to be associated with anemia among HIV patients in this study. This finding is in concordance with reports from Addis Ababa,¹⁵ Cambodia,¹⁹ and Iran.¹⁶ Zidovudine (ZDV) has long been identified to be a potential cause of anemia in HIV patients especially early in the ART initiation and with low baseline Hb.¹⁹

Additionally, a history of treatment with anti-TB drugs was significantly associated with anemia in our study. A similar finding was reported from the study done in Iraq.¹⁶ Since TB is known to cause anemia through various mechanisms including anemia of chronic illness, bone marrow involvement, malnutrition and hemoptysis, the presence of TB treatment history could partly suggest susceptibility of those HIV patients for anemia from the TB. Moreover, the anti-TB drugs such as isoniazid are identified to cause anemia.

The CD4+ T-lymphocyte count, <200 cells/μL, was found to be associated with anemia. Similar findings were reported by various studies in southern Ethiopia,^12,13 Cambodia,¹⁹ and South Africa.¹¹ The possible explanation for this association includes the fact that HIV patients with low CD4+ T-lymphocyte count are known to be at risk of multiple OIs, which are known to cause anemia, and the fact that such patients are also likely to have high HIV viral load which could lead to viral infiltration of the bone marrow subsequently causing anemia.

Since the causes and types of anemia in HIV patients are multi-factorial, we recognize the inability of this study to identify the specific types and specific causes of anemia as a limitation.

Conclusion and recommendation

Anemia continues to be a major co-morbidity among adult HIV patients in Ethiopia. Adult HIV patients who are taking ZDV-containing ART, with a history of TB treatment, have low CD4+T-lymphocytes count and ART-naïve should be carefully screened and treated for anemia. We recommend further longitudinal studies to determine predictors of anemia in the setting and intervention programs to change the existing situation.

Author contributions

HM conducted the study, participated in the data analysis, and interpreted the findings. HM, MMW, HW, AT, and NM participated in the design of the study, data analysis, drafting and revising the paper and agree to be accountable for all aspects of the work.

Acknowledgments

The authors thank University of Gondar for financing the study and Debre-Tabor Hospital for approving and facilitating the study.

Disclosure

The authors report no conflicts of interest in this work.