Pre-Exposure Prophylaxis (PrEP) in the Prevention of HIV: Strategies, Target Populations and Upcoming Treatments

Abstract

Pre-exposure prophylaxis (PrEP) against HIV infection with tenofovir/emtricitabine is proven to be effective yet uptake of this preventive measure has been difficult due to several barriers such as cost, access, and misinformation. Certain subpopulations are disproportionately affected by this problem. Several methods have been developed to close the gap in care. New antiretroviral treatment strategies are being investigated for safety and efficacy in preventing HIV infection.

Keywords:

• pre-exposure prophylaxis
• HIV prevention
• PrEP uptake
• medications

Introduction

Pre-exposure prophylaxis (PrEP) against human immunodeficiency virus (HIV) infection is recommended by the Centers for Disease Control and Prevention (CDC) for men who have sex with men (MSM), heterosexual men and women, and injection drug users at substantial risk of acquiring HIV infection. Substantial risk is defined as having an HIV-positive sexual partner, recent bacterial sexually transmitted infection (STI), high number of sex partners, history of inconsistent or no condom use, commercial sex work, HIV-positive injecting partner, or sharing injection equipment.¹

Tenofovir disoproxil fumarate/emtricitabine (TDF/FTC) was the first drug to receive Food and Drug Administration (FDA) approval for PrEP against HIV infection in July 2012. Results from the iPrEX and Partners Prep studies led to its approval, and it is currently recommended by the CDC for PrEP against HIV infection. In October 2019, tenofovir alafenamide/emtricitabine (TAF/FTC) also received FDA approval for PrEP however the indication excludes those at risk from receptive vaginal sex.² TAF/FTC for PrEP is not yet recommended in the United States (US) or European guidelines.^1,3 PrEP with TDF/FTC reaches maximum protection levels at about 7 days of use for people engaging in receptive anal sex. For patients engaging in receptive vaginal sex, PrEP reaches maximum protective levels at 21 days.⁴

Recently published data shows that there are 1.2 million people at high risk for acquiring HIV infection in the US but there were only 8768 PrEP utilizers in 2012. In 2016, this number increased to 77,120 which is a 73% increase in over a span of 4 years. States with the highest rate of PrEP use per 100,000 people were New York, Massachusetts, Rhode Island, Washington, and Illinois. Most PrEP users in 2016 were male and between the ages 25–44 (93% and 64%, respectively), and in the same year, 81% of all new HIV diagnoses were in men. More than half (54%) of all new diagnoses were ages 25–44 years. The South has the highest number of new HIV diagnoses compared to other regions of the country but has disproportionately lower number of people utilizing PrEP. More than half (52%) of all new HIV diagnoses in 2016 occurred in the South but only 30% of all PrEP users were from this region.⁵ These values likely underestimate PrEP uptake in the US because there is no central database that collects this information. Instead, researchers collect this data from pharmacies, insurance claims, hospitals, outpatient facilities, and physicians’ offices.

The World Health Organization (WHO) recommends PrEP for people at substantial risk of acquiring HIV infection as an additional preventive option.⁶ Globally, countries are at varying stages of PrEP uptake. It is estimated that there were 300,000 PrEP users in 2018 with most of them being in the US.⁷ A study conducted by UNAIDS showed that of the 69 countries that reported PrEP availability, 56 countries stated they had at least one barrier that limited patient access to PrEP. High out-of-pocket costs and PrEP only being provided in specialized HIV treatment locations were the top barriers to medication access.⁸

There is a gap in PrEP utilization with varying degrees of uptake in different countries. This narrative review article is intended to discuss strategies that have been used to increase PrEP utilization in the US, identify target populations in the US, and review medications that are currently under investigation for PrEP.

Methodology

The authors used the following search terms to search MEDLINE database to identify recently published review articles and clinical trials about upcoming treatment strategies for HIV PrEP: “HIV pre-exposure prophylaxis, ‘HIV pre-exposure prophylaxis regimens’, HIV pre-exposure prophylaxis ongoing clinical trials”. Ongoing trials were identified using the ClinicalTrials.gov website, which was searched with the following search terms: “HIV pre-exposure prophylaxis”. The search was limited to English-language articles as well as those published between 2015 and 2020, and to adults between 18 and 64 years old. No other search limits were applied.

Relevant articles were defined as those involving HIV regimens and strategies that are used for prophylactic purposes. Excluded studied were those that focused on pediatric patients or those that evaluated regimens used for treatment. We also excluded studies that evaluated the currently established pre-exposure prophylaxis regiments. Studies addressing social, administrative, cultural, or financial aspects of pre-exposure prophylaxis, or those that evaluated non-drug prophylactic measures, were excluded as well. For a more complete perspective, studies involving male and female populations, as well as pregnant patients were included. Conference abstracts were included as well. The MEDLINE literature search using the term “HIV pre-exposure prophylaxis” yielded 381 articles, all of which described either the established regimens, non-drug measures, or social/cultural patient factors related to pre-exposure prophylaxis. The search narrowed to search term “HIV pre-exposure prophylaxis regimens” yielded 5 articles, of which one was deemed relevant to this review. The ClinicalTrials.gov search yielded 10 articles for the search term “HIV pre-exposure prophylaxis” with the applied filters for recruiting, active not recruiting, and complete studies that are interventional, Phase III, and for the adult population (18 to 64 years old).

Additional sources of information and references were identified through the review of cited references. Article selection was based on mutual agreement for inclusion.

Strategies

Multiple layers of barriers exist resulting in slow uptake of PrEP with tenofovir/FTC. These barriers include provider-level, patient-level, and health-systems-related or structural barriers. Provider-level barriers include lack of training in PrEP prescribing guidelines, identifying high-risk patients, how to monitor for efficacy and toxicity after patients start taking the antiretrovirals (ART), and insurance coverage. Many are also unclear as to whether primary care physicians or infectious disease physicians should prescribe PrEP to patients. Infectious disease providers are trained to deliver healthcare to patients with HIV infection and are well equipped to prescribe PrEP; however, their practice is limited to patients with established HIV infection. Primary care providers regularly care for patients without HIV infection but may lack the training necessary to prescribe PrEP. This “purview paradox” is a significant obstacle standing in the way between patients and the medications they need. Patient-level barriers include geographic location and inability to access PrEP providers, conspiracy beliefs, stigma, being uninsured or underinsured, immigration status, and competing medical or behavioral priorities such as existing medical conditions or substance abuse. Barriers in health-systems include inflexible appointment times that do not accommodate for work schedules, time constraints during appointments, and insufficient number of PrEP providers.^9–13 These barriers need to be overcome so persons at high risk of acquiring HIV infection can access PrEP for as long as they need it.

One of the ways to increase access to PrEP is to allow community pharmacies to open PrEP clinics. Pharmacists working in the community are in the unique position to screen high-risk patients, offer PrEP counseling, prescribe and dispense tenofovir/FTC with a collaborative drug therapy agreement (CDTA) with a physician. CDTA is an agreement between a physician and pharmacist that allows the pharmacist to initiate, modify, or discontinue drug therapy under the guidance of a protocol for a patient without the patient needing to see the physician.¹⁴ It is easier to access a pharmacist than it is to access a physician. A study conducted by Tung and colleagues saw that a pharmacist-run PrEP clinic was logistically feasible, financially viable, and had high patient retention rates (75%) after one year of care. There were 251 persons evaluated for PrEP and 245 of them initiated PrEP. Most (96%) did not pay a co-pay for their PrEP regimen.¹⁵ Long-term data from the same study showed that 74% of individuals were able to start their PrEP regimen on the same day as their initial appointment. Information from medication refill dates showed that 90% of patients had a mean proportion of days covered greater than 80%. There were zero HIV seroconversions.¹⁶ Allowing pharmacists to open PrEP clinics through CDTAs with physicians will remove barriers for patients interested in PrEP by increasing the number of locations that are available to them. Seeking PrEP services from a pharmacist in a community pharmacy setting may also reduce a person’s anxiety, because pharmacies are not directly associated with a single health condition or disease and therefore are free of PrEP or HIV stigma.¹⁷ In late 2019, California Governor Gavin Newson signed new legislation giving pharmacists the authority to dispense 30 to 60-day supply of PrEP without a prescription or a CDTA with a physician. Pharmacists will be required to complete a training program before being allowed to dispense antiretrovirals for this indication. California is the first state to implement this change in the United States.¹⁸ A study conducted in Mississippi piloted a novel method for same-day PrEP administration. They allowed pharmacists to screen people for PrEP and assess for contraindications. If a person was found to not have any contraindications to PrEP, a prescription for antiretrovirals was written without checking baseline labs. In addition, the person was given an appointment within 6 weeks to see a clinician for further assessment and labs. Most (83%) patients received a same-day prescription for PrEP and 97% received within 5 days. Of those who received a prescription, 77% filled the prescription and 43% of them attended their first appointment within 6 weeks.¹⁹ Similar pharmacist-led PrEP initiatives with positive results can be seen in other trials.^20–22 While expanding the role of a pharmacist does not remove all barriers to PrEP, it certainly can ease access.

Persons living in rural areas are challenged with a lack of providers close to their area of residence. Commute times and lack of transportation can pose substantial difficulties in accessing and continuing PrEP for those at high risk of acquiring HIV infection. In one research study, epidemiologists reported 1 in 8 (108,758/844,574; 13%) PrEP-eligible MSM lived at least 30 minutes away from the nearest PrEP provider.²³ This problem can be mitigated with the use of telemedicine technologies. In a study done by Refugio and colleagues, 25 HIV-negative young men who have sex with men (YMSM) of color received PrEP services through telemedicine. At least 75% of them agreed that receiving PrEP through telemedicine technologies was fast, convenient, easy to use, and confidential. Less than 15% of the study population felt stigmatized for using PrEP. There were no HIV seroconversions during the study.²⁴ In another study in the state of Iowa, pharmacists employed telemedicine to conduct patient interviews, order laboratory studies, and mail PrEP medications to patients. Study results showed that 91% of video visits resulted in PrEP initiation. Retention rate at 6 months was 61% and 96% of patients completed laboratory monitoring. There were 37 cases of sexually transmitted infections (STIs) that were diagnosed.²¹ Another study comparing retention rates among persons receiving PrEP services via telehealth vs standard in-person visits did not have as favorable results. There was no significant difference in PrEP prescription rates, first month follow-up visit, or medication adherence rate at 1 month. There were significantly less number of patients who attended their 3-month follow-up visit in the telemedicine arm compared to the standard visit arm (40% vs 87%; p=0.05).²⁵ Although telemedicine eases access to PrEP services, it lacks the personalization of in-person visits. This may not be important to some patients but for others, it can be the difference between staying in care or being lost to follow-up. Offering both in-person and telehealth to visits to patients when discussing PrEP might give patients more flexibility to choose a method tailored to their needs.

The use of short message service (SMS), online web resources, and mobile apps designed to increase PrEP awareness, education, access, and adherence may also be a valuable tool to engage people at high risk for HIV transmission. Investigators compared rates of retention and PrEP adherence among persons using an online platform called PrEPmate plus standard care to those only receiving standard care. PrEPmate includes an interactive online platform where educational content is shared with consumers and SMS. Daily and weekly SMS are sent to promote adherence, answer questions, and confirm appointments. There was a statistically significant difference in adherence (PrEPmate 72%, standard 57%, p=0.03), defined as tenofovir concentration ≥700 fmol/punch, and retention rates (PrEPmate 86%, standard 71%, p=0.01) among participants who used PrEPmate compared to standard of care.²⁶ Another study investigated the use of a mobile app designed to provide various services to MSM. The app is comprised of self-assessment tools, screening questionnaire for PrEP, HIV prevention recommendations, list of PrEP providers by location, and the ability to order condoms and at-home HIV testing kits. The authors found that in a 4-month period, the app was used an average of 17.7 minutes. More than half of the participants used the app to order condoms (77/121, 63.6%) and testing kits (64/121, 52.8%) and 9% (8/86) of PrEP-eligible participants started PrEP.²⁷ While the percentage of MSM who started PrEP does not seem significant, it is still eight more individuals on PrEP than there were prior to the use of the app. None of these interventions alone will dissolve all barriers or convince all persons that need it to start PrEP. Increasing PrEP uptake can be done by offering candidates a combination and/or a variety of interventions and choices personalized to meet their specific needs.

Patient- and provider-level PrEP stigma is an important barrier to address in order to combat poor PrEP uptake. Patients often times feel uncomfortable disclosing their sexual history to those closest to them or their healthcare providers for fear of being judged or discriminated against. PrEP users can be misunderstood to have HIV infection as medications used to treat and prevent HIV are the same. In addition, PrEP users can be labeled as promiscuous resulting in rejection by partners or loved ones.^28–30 Language used in promotional materials, educational pamphlets, and multimedia outlets can perpetuate the implicit and explicit biases readers have against PrEP and PrEP users. Some of these biases are that a patient taking PrEP leads a “high-risk” lifestyle and has unconventional sexual practices when the fact is, taking PrEP as prescribed reducing risk of HIV transmission and promotes accountability for one’s own health and the health of the partner.³¹ Words and terms such a “promiscuous”, “unprotected sex”, “infected” and “medication noncompliance” can be alienating and judgmental.³² Instead of focusing on the negative connotations associated with PrEP and its use, all parties involved in the PrEP continuum should discuss its positive implications such as taking responsibility for the health of oneself and others through self-advocacy and empowerment. A meta-analysis on health message framing found that gain-framed messages about prevention behaviors were more likely to be successful in encouraging patients to make positive changes than loss-framed messages.³³ Gain-framed messaging emphasizes the benefits of starting a healthy behavior or taking a medication whereas a loss-framed messages highlight what the patient will lose if they fail to engage in said behavior or take a medication. For example, a gain-framed message about PrEP could be “Taking once-daily tenofovir/FTC will reduce your risk of acquiring HIV infection.” Conversely, a loss-framed message will state the same message but in a negative context: “Not taking once-daily tenofovir/FTC will increase your risk of acquiring HIV infection.” Gain-framed messages will also reduce the stigma around taking PrEP medications and thereby weaken one more barrier for patients.

Healthcare providers can unwittingly perpetuate stigma as well. There is fear within the healthcare provider community that PrEP use will be a gateway for riskier behavior.^31,34 Multiple analyses have shown that risk of HIV transmission is significantly lowered when PrEP is taken as prescribed.^35–37 Withholding PrEP from individuals that can benefit from it would be unethical and may increase HIV transmission rates. Educating the medical community, especially primary care providers who are at the forefront of PrEP implementation, can catalyze the scalability of PrEP.

On a policy level, there are still 14 states in the US that have not expanded Medicaid which was a service provided by the Affordable Care Act (ACA) that passed into law in 2010. A supreme court ruling in 2012 gave state governance the option to expand Medicaid coverage or leave it as is. People with low-income that live in one of these 14 states have difficulty paying for PrEP services and medications.³⁸ Medicaid expansion in all 50 states would remove the financial burden of starting PrEP in people with low-income.

The states of New York, Massachusetts, and Washington in the United States have adopted PrEP patient assistance programs for those that are uninsured or underinsured. Prescribers registered with the program will be reimbursed by the state for services such as HIV testing, counseling, STD testing, and supportive care services. PrEP medication is not covered through this state program but eligible patients can receive tenofovir/FTC for free through the manufacturer’s (Gilead) patient assistance program. An individual is considered eligible for Gilead Advancing Access Medication Assistance Program if he/she is uninsured and the household income is 500% Federal Poverty Level (FPL) regardless of immigration status.³⁹ These state and private programs offset the cost of PrEP care for patients.^14,40 The availability of such programs is invaluable to patients with financial barriers and can significantly impact PrEP uptake. In fact, the 3 states with patient assistance programs are among the top 5 states with the highest PrEP uptake rates in the US.⁴¹ Table 1 contains a summary of the strategies discussed in this article.

Table 1 Strategies to Increase PrEP Uptake^9–41

Barrier	Strategies to Implement
“Purview paradox”	Educating primary care providers (PCP) about their role in preventing HIV transmission and PrEP prescribing Empowering PCPs to discuss PrEP with their patients by providing them with the necessary tools and resources such as allocating appointment time slots for PrEP candidates and furnishing the exam rooms with educational pamphlets Expanding the role of infectious disease providers to provide services to PrEP candidates
Transportation barriers and PrEP provider deserts	Use telemedicine technologies to interview patients, order laboratory monitoring, and prescribe PrEP Change legislature to allow pharmacists to test for HIV and prescribe PrEP regimens; arrange CDTAs between pharmacists and physicians so pharmacists can operate PrEP clinics, counsel patients, order laboratory monitoring, and initiate/modify/discontinue PrEP Utilize apps, websites, and SMS to interact with patients in a virtual platform and keep them informed about their appointments, medications, and general information about PrEP
Patient- and provider-level stigma	Gain-framed health messaging and reduce stigma and increase patient buy-in Educate healthcare providers of the benefits of PrEP in reducing HIV transmission Create educational material with positive messaging and remove verbiage that further stigmatize the use of PrEP such as “promiscuous”, “high-risk”, “infected”, “unprotected sex”, “medication noncompliance”.
Financial burden of PrEP	Increasing Medicaid expansion to all states in the US Creating private and state level patient assistance programs for PrEP in order to offset cost to providers and patients

Target Populations

Target population that can benefit from PrEP includes individuals at high-risk of acquiring HIV. Men who have sex with men (MSM) account for the largest proportion among other subpopulation, leading most prevention efforts to focus on identifying HIV risk factors among MSM. Within the MSM population, black and Hispanic MSM are disproportionately affected by the slow uptake of PrEP.^42,43 A study in Atlanta, USA surveyed 436 black MSM about PrEP awareness. Black MSM unaware of PrEP had lower rates of HIV testing knowledge, less HIV testing rates, and higher rates of transactional sex. This study demonstrated that those that were at highest risk for HIV infection had lower rates of PrEP awareness and HIV testing.⁴⁴

One study developed a multivariable prediction model developed using the medical records from a Sexually Transmitted Disease (STD) clinic estimated a higher likelihood of acquiring HIV among the MSM with a diagnosis of STD and substance use. The model also constructed a risk score of HIV acquisition and suggested the clinicians use it to identify candidates for intensive prevention activities such as PrEP. The clinicians can calculate patient’s risk score (ranged 0 to 19) based on patient’s answers to four questions, find out patient’s estimated 4-year HIV risk using the chart provided, and develop a follow-up plan such as testing recommendation or prevention intervention.⁴⁵ Another risk score to screen high-risk MSM was developed using a clinical trial data on MSM who had not used injection drugs.⁴⁶

In addition, heterosexual men and women who practice risky sexual behaviors are candidates of PrEP. For example, individuals having unprotected sex or having multiple sex partners are more likely to acquire HIV.^47,48 In addition, likelihood of being exposed to a HIV-infected partner is greater in the communities that are known to have a high HIV prevalence such as non-Hispanic black community or low-income population.^49–51 Moreover, commercial sex workers and prison inmates are known to be high-risk heterosexual subgroups that would need intensive prevention activities.⁵² Therefore, the efforts to increase PrEP uptake could be prioritized to these communities to reduce a risk of HIV exposure.

PrEP Medication Regimens Under Investigation

The National Institute of Allergy and Infectious Diseases (NIAID) is evaluating the efficacy of long-acting cabotegravir compared to TDF/FTC in HIV-uninfected women who are at risk for HIV. This is a randomized parallel assignment study with two multi-step treatment arms: oral cabotegravir, oral TDF/FTC, and long-acting IM cabotegravir. The primary outcome measures are the number of documented HOV infections as well as number of adverse events. The study is currently recruiting eligible patients throughout several African locations, has an estimated enrollment of 3200 participants, and is expected to be completed in May 2022.⁵³ The NIAID is currently recruiting participants for a study evaluating the safety and efficacy of cabotegravir for PrEP in HIV-uninfected cisgender men and transgender women who have sex with men. Study participants will be randomized into 1 of 2 arms: (1) daily oral CAB tablets and daily oral TDF/FTC placebo tablets for 5 weeks (step 1) IM injection of CAB at 2 time points 4 weeks apart and every 8 weeks thereafter and daily oral TDF/FTC placebo tablets to week 153 (step 2); (2) daily oral TDF/FTC tablets and daily oral CAB placebo tablets for 5 weeks (step 1) daily oral TDF/FTC tablets and an IM injection of placebo at 2 time points 4 weeks apartment and every 8 weeks thereafter to week 153 (step 2). Step 3 involves all participants in both arms receiving daily oral TDF/FTC tablets starting week 153 and continuing for 48 weeks. Primary outcomes measures are number of documented incident HIV infections and number of Grade 2 or higher clinical and laboratory adverse events. The estimated study completion date is March 2022.⁵⁴

Gilead Sciences is currently conducting a Phase III, randomized, double-blind study to evaluate the efficacy and safety and emtricitabine and tenofovir alafenamide (F/TAF) fixed-dose combination once-daily regimen, for pre-exposure prophylaxis in men and transgender women who have sex with men and are at risk of HIV-1 Infection. This study is ongoing with anticipated completion date in September 2021.⁵⁵

The International Partnership for Microbicides (IPM) guided two studies that evaluated the efficacy of a dapivirine vaginal ring. The first trial, titled “The Ring Study”, was completed in 2016 and showed a 31% decreased incidence of HIV-1 infection in HIV negative, healthy women, and was not associated with any safety concerns.⁵⁶ These findings were also confirmed in a separate NIAID study, ASPIRE, as well as National Institute of Health (NIH)-supported HOPE study.^57,58 The second IPM-led study, the DREAM trial, was an extension trial that took place at six former Ring Study locations.⁵⁹ It was completed in January 2019, and confirmed the results of the Ring Study with a 63% HIV risk reduction in women. The ring device is currently under European regulatory agency review, with plans to seek FDA approval in the US.⁶⁰

NIAID is currently recruiting participants for another extension study that will investigate the safety of and adherence to dapivirine vaginal ring in adolescent and young female population. This is a Phase IIa randomized, open-label, crossover study with the following outcome measures: frequency of adverse events, detectable drug levels in blood, and percentage of participants reporting acceptability and preference for dapivirine vaginal ring. The study is expected to be completed in November 2021.⁶¹

The HIV Vaccine Trials Network and the HIV Prevention Trials Network are also currently investigating a new preventive strategy that is related to what has been done in HIV vaccine research.⁶² The Antibody Mediated Prevention Study will investigate the direct intravenous infusion of VRC01 antibody, which was previously studied and as a treatment option in the Red Cross Research Center in Bangkok, Thailand. The trial showed that the antibody was well tolerated; however, it did not significantly increase the number of participants with viral suppression, prompting further research in this field.⁶³

Another strategy investigated as PrEP is a reservoir-style implant for sustained release of TAF as a novel formulation for antiretroviral drug delivery and prophylaxis.⁶⁴ Pharmacokinetic studies so far have shown sustained plasma TAF concentrations, as well as in-vivo TAF release rate of active implants that correlated well with the in-vitro TAF release from similar devices. This makes the TAF device suitable for continued development as a long-acting subcutaneous implant for HIV PrEP.⁶⁵

The University of KwaZulu is conducting an open-label, randomized control study on the safety of TDF/FTC in PrEP during pregnancy and lactation. Pregnant women at risk for HIV will be randomized to one of two arms in which participants are either initiated on PrEP in pregnancy and continue using TDF/FTC throughout breastfeeding or PrEP is deferred until breastfeeding cessation. The two arms are (1) standard HIV prevention strategy plus once-daily TDF/FTC initiated in pregnancy and continued until breastfeeding cessation or 18 months after delivery, whichever is earliest and (2) standard HIV prevention strategy throughout pregnancy until breastfeeding cessation. The primary outcomes measures are pregnancy outcome-preterm deliveries, bone mineral density in mother and infant, and pregnancy outcome-low birth weight. Other outcomes include incident HIV infections and adherence to PrEP. This study is estimated to be completed in May 2022.⁶⁶ A study by the Kirby Institute is evaluating the incidence of HIV among PrEP study participants and population-level impact of a rapid roll-out of PrEP on HIV diagnoses amongst gay and bisexual men over a 2-year period. This is a single open-label arm study in which all participants will receive daily oral TDF/FTC. The primary outcome measures are incidence of HIV infection per 100-person years among study participants and number of HIV diagnoses among gay and bisexual men notified to the New South Wales Ministry of Health. The study is estimated to be completed in October 2020.⁶⁷ Table 2 contains a summary of drugs under investigation.

Table 2 Summary of PrEP Regimens/Strategies Under Investigation

Authors/Sponsor	Title	Study Design	Studied Regimen	Study Objective	Status of the Study	Results (Where Available)
Gilead Sciences^Citation55	A Phase III, Randomized, Double-blind Study to Evaluate the Safety and Efficacy of Emtricitabine and Tenofovir Alafenamide (F/TAF) Fixed-Dose Combination Once Daily for Pre-Exposure Prophylaxis in Men and Transgender Women Who Have Sex With Men and Are At Risk of HIV-1 Infection	Randomized, parallel assignment, double blind, placebo controlled clinical trial with 5400 participants	Daily emtricitabine/tenofovir alafenamide (F/TAF) or emtricitabine/tenofovir DF (F/TDF)	To assess the rates of HIV-1 infection in MSM and transgender women who have sex with men	Phase III, ongoing	N/A
The National Institute of Allergy and Infectious Diseases (NIAID)^Citation53	Evaluating the Safety and Efficacy of Long-Acting Injectable Cabotegravir Compared to Daily Oral TDF/FTC for Pre- Exposure Prophylaxis in HIV-Uninfected Women	Randomized, quadruple blinded, parallel assignment clinical trial with 3200 participants	Long-acting injectable integrase inhibitor cabotegravir (CAB LA) vs oral daily tenofovir disoproxil fumarate/emtricitabine (TDF/FTC)	Evaluate the safety and efficacy of the long-acting injectable agent cabotegravir (CAB LA) compared to daily oral tenofovir disoproxil fumarate/emtricitabine (TDF/FTC) for pre-exposure prophylaxis (PrEP) in HIV-uninfected women	Phase III, Recruiting	N/A
The International Partnership for Microbicides (IPM)^Citation56	Open-label Study of IPM’s Dapivirine Vaginal Ring Show Increased Use and Suggest Lower Infection Rates Compared to Earlier Phase III Study	Open-label study conducted in South Africa and Uganda that followed 941 women ages 20–50 for 12 months. The	Dapivirine vaginal ring	To collect additional safety data and information on how women who participated in an earlier Phase III trial, used the ring	Completed; under review by European Medicines Agency	Increased use of the ring and 63% reduction in HIV risk
The National Institute of Allergy and Infectious Diseases (NIAID)^Citation61	Evaluating the Safety of and Adherence to a Vaginal Matrix Ring Containing Dapivirine and Oral Emtricitabine/Tenofovir Disoproxil Fumarate in an Adolescent and Young Adult Female Population.	Randomized, open-label, crossover trial with 300 female patients ages 16–21 years	Dapivirine vaginal ring vs FTC/TDF	To evaluate the safety of and adherence to a vaginal matrix ring (VR) containing dapivirine and oral FTC/TDF	Phase IIa	N/A
The HIV Vaccine Trials Network and the HIV Prevention Trials Network^Citation62	The Antibody-Mediated Study	N/A	Direct intravenous infusion of VRC01 antibody	To study the response to a direct infusion of VRC01 antibody	Enrollment completed	N/A
University of KwaZulu^Citation66	Immediate or Deferred Pre-exposure Prophylaxis for HIV Prevention: Safe Options for Pregnant and Lactating Women (PrEP)	An open-label randomized control study with estimated enrollment of 842 pregnant women considered at high risk for HIV infection	Standard HIV prevention strategy plus once daily TDF/FTC initiated in pregnancy and continued until breastfeeding cessation or 18 months after delivery, whichever is earliest vs standard HIV prevention strategy throughout pregnancy until breastfeeding cessation	To explore the safety of TDF/FTC when used as PrEP during pregnancy and lactation	Phase II and III, recruiting	N/A
National Institute of Allergy and Infectious Diseases (NIAID) And ViiV Healthcare and Gilead Sciences^Citation54	Safety and Efficacy Study of Injectable Cabotegravir Compared to Daily Oral Tenofovir Disoproxil Fumarate/Emtricitabine (TDF/FTC), For Pre-Exposure Prophylaxis in HIV-Uninfected Cisgender Men and Transgender Women Who Have Sex With Men	Randomized, double-blind, parallel with estimated enrollment of 5000 HIV-uninfected cisgender men and transgender women who have sex with men	2 arms: (arm 1) daily oral CAB tablets and daily oral TDF/FTC placebo tablets for 5 weeks (step 1) IM injection of CAB at 2 time points 4 weeks apart and every 8 weeks thereafter and daily oral TDF/FTC placebo tablets to week 153 (step 2); (arm 2) daily oral TDF/FTC tablets and daily oral CAB placebo tablets for 5 weeks (step 1) daily oral TDF/FTC tablets and an IM injection of placebo at 2 time points 4 weeks apartment and every 8 weeks thereafter to week 153 (step 2). Step 3 involves all participants in both arms receiving daily oral TDF/FTC tablets starting week 153 and continuing for 48 weeks.	To evaluate the safety and efficacy of cabotegravir for PrEP in HIV-uninfected cisgender men and transgender women who have sex with men	Phase IIb/III, recruiting	N/A
Kirby Institute^Citation67	Expanded PrEP Implementation in Communities in NSW (EPIC-NSW)	Single, open-label arm with 9733 participants enrolled (adults at high risk of HIV, mostly gay and bisexual men)	One pill daily TDF/FTC	To assess the incidence of HIV among PrEP study participants and measure the population-level impact of the rapid roll-out of PrEP on HIV diagnoses among gay and bisexual men in NSW over a two-year period.	Phase III, active, not recruiting	N/A

Conclusion

The uptake of PrEP among high-risk patient groups is critical in fighting the HIV epidemic. Identifying target populations and utilizing different strategies to combat barriers is needed to increase access to PrEP services. Research in alternative PrEP methods is ongoing.

Disclosure

The authors report no conflicts of interest in this work.