Prevalence of anemia and nutritional status among HIV-positive children receiving antiretroviral therapy in Harar, eastern Ethiopa

Abstract

Purpose

Anemia and growth retardation are common manifestations of HIV-positive children, which threaten their lives. Therefore, this study tried to assess the burden of anemia and the nutritional status of HIV-positive children receiving antiretroviral therapy (ART) in eastern Ethiopa.

Patients and methods

A total of 108 records of children on ART followed up in Hiwot Fana Specialized University Hospital from 2007 to 2010 were retrospectively reviewed from November 1 to November 30, 2011.

Results

Approximately 54.4% of the children had been anemic before the initiation of their ART (at baseline): 7.8% were severely anemic and 44.7% were moderately anemic. These percentages were higher in preschool children than in school children (adjusted odds ratio [AOR]: 4.80 [95% confidence interval {CI}: 1.96, 11.75]), and were higher in males than in females (AOR: 2.61 [95% CI: 1.06, 6.45]). The prevalence of anemia was reduced to 39.2% 1 year after initiation of ART. The increasing of hemoglobin values was highly significant for both zidovudine (AZT)- and stavudine (d4T)-based ART (P<0.05). At baseline, 51.6% of the study subjects were underweight (weight-for-age Z score less than −2 standard deviation [SD]); 49.1% were stunted (height-for-age Z score less than −2 SD); and 31.5% were wasted (body mass index less than −2 SD), which, after a year on ART, declined to 8.9%, 15.9%, and 9.8%, respectively.

Conclusion

There was high prevalence of anemia and growth failure among HIV-infected children in the study area. However, there was a decline after initiation of ART. Therefore, adherence counseling to strengthen the uptake of ART is recommended. Moreover, large-scale, prospective studies should be done to understand the magnitude and etiology of the problems with HIV-negative control groups.

Keywords:

• hemoglobin
• underweight
• stunting
• wasting

Introduction

Human immunodeficiency virus (HIV) epidemic remains a serious challenge and continues to take its toll, particularly on vulnerable populations such as children. More than two out of three (68%) adults, nearly 90% of HIV-infected children, and more than three-quarters (76%) acquired immunodeficiency syndrome (AIDS) deaths in 2007 occurred in Sub-Saharan Africa.¹

Anemia is a common manifestation of pediatric HIV infection and is a significant negative predictor of survival.^2,3 It occurs in 50%–90% of children living in both resource-limited and resource-rich settings.⁴ Many etiological factors probably contribute to the development of low iron status in HIV-infected children, such as reduced dietary intake, the quality of dietary iron, and altered iron absorption.⁵

Malnutrition is an important factor which might predict disease progression of HIV-infected individuals. It also results in higher risk of morbidity and mortality in both HIV-infected adults and children. Wasting and weight loss are common features of HIV infection, especially in resource-limited settings. In one review paper, approximately 40%–44% of adult wasting and 59% of child malnutrition were reported.⁶

Although there are studies on anemia and the nutritional status of HIV-infected children in Ethiopia,⁷ there have been no studies done in the eastern part of the country. Therefore, this study tried to assess the burden of anemia and the nutritional status of HIV-positive children receiving antiretroviral therapy (ART) in eastern Ethiopa.

Methods

Study area, design, and study period

A retrospective record review was conducted from November 1 to November 30, 2011, on HIV-positive children who attended for ART from 2007 to 2010 at the Hiwot Fana Specialized University Hospital. The hospital is located in Harar (the capital of Harari National Regional State), Ethiopia, which is 511 km from the city of Addis Ababa. The facility began providing ART service in 2007; since then, 183 children have attended to receive the treatment.

Population

The source population included all HIV-positive children who visited Hiwot Fana Specialized University Hospital for medical care. The study population included all HIV-positive children who received ART services in the ART unit of Hiwot Fana Specialized University Hospital.

Sample size and sampling techniques

All the children registered for ART in the hospital from the very beginning of the service provision were considered. Those who started ART clinically (20 children), those who dropped out early (15 children), those who transferred into the ART program (6 children), those who transferred out of the program early (12 children), and those whose records were incomplete (22 children) were excluded from the study; resulting in 108 children included in the study. Figure 1 flow chart shows how the study participants were selected.

Figure 1 Flow chart depicting selection process for recruitment of study participants.

Abbreviation: ART, antiretroviral therapy.

Data collection methods

Data were collected by nurses who were working in the ART unit of the hospital. A checklist, which was developed from the children ART monitoring record book, was used to collect data from ART record books on sociodemographic status, hemoglobin, weight, height, CD4T cell count, type of ART regimens used, World Health Organization (WHO) clinical stages, tuberculosis (TB) screening results, and other items at baseline (before initiation of ART) and 1 year after ART initiation.

Data analysis

Data were entered into SPSS version 15, cleaned, and analyzed. Children aged less than 7 years were taken as preschool age, while those above 7 years were identified as school children. Anemia was defined as a hemoglobin concentration of less than 11 g/dL for ages less than 5 years, <11.5 g/dL for ages 5–11 years, and less than 12 g/dL for ages 12–14 years. In addition, severe anemia was defined as a hemoglobin concentration of less than 7 g/dL.⁸

The prevalence of anemia was determined as the proportion of anemic children. Immunodeficiency was classified into the three categories of “no evidence”, “moderate”, and “severe” immune suppression according to the 1994 revised classification system for HIV infection in children less than 13 years of age.⁹ Nutritional status of the children was analyzed using the WHO Anthroplus software and was expressed as standard deviation (SD). Weight-for-age Z score (WAZ), height-for-age Z score (HAZ), and body mass index (BMI) scores of less than −2 SD were considered as underweight, stunting, and wasting, respectively. Those children with a WAZ, HAZ, or BMI score less than −3 SD and between −3 SD and −2 SD were considered as severely and moderately underweight, stunting, and wasting, respectively.^10,11 The prevalence of anemia was cross-tabulated with different study variables. Association of the variables was checked by using Pearson’s chi–square and Fisher’s exact tests, with P-values less than 0.05 at a 95% confidence interval (CI) taken as a statistically significant difference. Paired t-tests were used to compare an increase in hemoglobin and CD4 values after ART. Univariate and multivariate logistic regressions were employed to identify the possible risk factors of anemia.

Ethical consideration

This study obtained ethical clearance from the Institutional Health Research and Ethics Review Committee of the Haramaya University, Harar, Ethiopia. Information that was obtained during this study was kept confidential.

Results

Study participants

A total of 108 children participated in this study, of whom 55.6% were female, 50% were preschool children, and approximately 92.6% were urban dwellers. Their mean age was 7.0 years (SD ±3.2), with a range of 1.0–12.0 years. On average, children attended ART for 40 months (SD ±16.0), with a range of 13.0–74.0 months. Few participants presented with smear-positive TB (13.9%). Based on WHO clinical stages, 58.7% were in stage 3, while 5.8%, 32.7%, and 2.9% of them were in stages 1, 2, and 4, respectively. Approximately 89.2% (91/102) of them were immunosuppressed before initiation of ART, out of which 66.7% were severely and 22.5% were moderately immunosuppressed. Most of the children (63.9%) were on a stavudine (d4T)-lamivudine (3TC)-nevirapine (NVP) ART regimen, while 4.6%, 23.2%, and 8.3% of them were on d4T- 3TC-efavirenz (EFV), zidovudine (AZT)-3TC-NVP, and AZT-3TC-EFV, respectively. A total of 107 children had CD4 levels at baseline with mean of 336.78 (SD ±349.34). The overall level of adherence to ART was 99.1%.

Prevalence of anemia

At baseline, the mean of the study subjects’ hemoglobin was 10.7 g/dL (SD ±2.0), ranging from 5.8 to 15.0 g/dL. The prevalence of anemia among children was 54.4% (56/103). Of anemic children, 44.7% were moderately and 7.8% were severely anemic. Anemia was higher among preschool than school children (P<0.01). It was also higher among male, WHO clinical stages 3 and 4, TB-negative, urban, severely immunosuppressed, and cotrimoxazole treatment groups. However, the prevalence of anemia was not statistically significant with the above variables (P>0.05) (Table 1).

Table 1 Prevalence of anemia before initiation of ART among HIV-infected children attending ART in Hiwot Fana Specialized University Hospital, Harar, eastern Ethiopa, 2011

Variables	Anemic, N (%)	Non-anemic, N (%)	P-value
Age (years)
Preschool (<7)	36 (35)	14 (13.6)	<0.01
School (≥7)	20 (19.4)	33 (32.0)
Sex
Male	30 (29.1)	17 (16.5)	0.07
Female	26 (25.2)	30 (29.2)
Residence
Urban	51 (49.5)	44 (42.7)	0.63
Rural	5 (4.9)	3 (2.9)
WHO clinical stage
1 and 2	23 (23.2)	15 (15.2)	0.35
3 and 4	31 (31.3)	30 (30.3)
Tuberculosis infection
Positive	8 (7.8)	7 (6.8)	0.51
Negative	49 (47.6)	39 (37.9)
Cotrimoxazole treatment
Yes	55 (53.4)	47 (45.6)	1.00*
No	1 (1.0)	0 (0%)
Immunosuppression
No evidence	4 (4.1)	7 (7.2)	0.26
Moderate	11 (11.3)	12 (12.4)
Severe	38 (25.8)	25 (39.2)

Note:

* Fisher’s exact test result.

Abbreviations: ART, antiretroviral therapy; HIV, human immunodeficiency virus; N, number of patients; WHO, World Health Organization.

The prevalence of anemia was higher in those children who were underweight, and stunting but were not in no wasting group before the initiation of ART. However, it was not statistically significant when comparisons were made for the above nutritional assessment variables (P>0.05) (Table 2).

Table 2 Prevalence of anemia in relation to nutritional status before initiation of ART among HIV-infected children attending ART clinics at Hiwot Fana Specialized University Hospital, Harar, eastern Ethiopa, 2011

Variables	Anemic, N (%)	Non-anemic, N (%)	P-value
Weight-for-age Z score
Underweight	29 (32.2)	19 (21.1)	0.44
Not underweight	22 (24.4)	20 (22.2)
Height-for age Z score
Stunting	31 (30.1)	22 (21.4)	0.39
No stunting	25 (24.3)	25 (24.3)
Body mass index
Wasting	15 (14.6)	17 (16.5)	0.31
No wasting	41 (39.8)	30 (29.1)

Abbreviations: ART, antiretroviral therapy; HIV, human immunodeficiency virus; N, number of patients.

The mean hemoglobin levels before and after initiation of ART were 11.1 (SD ±1.9) and 12 (SD ±1.8), respectively. The difference was statistically significant (P<0.05), with t=−5.84 (CI: −1.70, −8.37). The prevalence of anemia declined from 60.8% to 39.2% 1 year after initiation of ART (P<0.05). The prevalence of anemia 1 year after ART began was not related to AZT-based medication (P>0.05). The average increases in hemoglobin upon treatment with AZT- and d4T-based ART were 1 g/dL and 0.9 g/dL, respectively. The difference was statistically significant in those children with both AZT- and d4T-based treatments (P<0.05). The mean CD4 count before and after initiation of ART was 378.76 (SD ±405.18) and 710.71 (SD ±475.83), respectively. The difference was statistical significant (P<0.05), with t=−4.39 (CI: −484.85, −179.05) (Table 3).

Table 3 Prevalence of anemia and average hemoglobin level before and 1 year after initiation of ART among HIV-infected children attending ART clinics in Hiwot Fana Specialized University Hospital, Harar, eastern Ethiopa, 2011

Variables	Before ART, N=66	One year after ART, N=66	P-value
Anemic	31 (60.8%)	20 (39.2%)	0.04
Anemia
AZT-based		7 (10.6%)	0.85
d4T-based		13 (19.7%)
Mean hemoglobin
AZT-based (N=22),	11	12	0.04
mean (SD)	(SD ±2.0)	(SD ±1.5)
d4T-based (N=44),	11.1	12	<0.01
mean (SD)	(SD ±1.9)	(SD ±1.7)
Mean CD4 count
AZT-based (N=12),	226.41	598.25	0.01
mean (SD)	(SD ±134.41)	(SD ±464.45)
d4T-based (N=27),	508.15	760.70	0.02
mean (SD)	(SD ±523.15)	(SD ±480.84)

Abbreviations: ART, antiretroviral therapy; AZT, zidovudine; d4T, stavudine; HIV, human immunodeficiency virus; N, number of patients; SD, standard deviation.

Nutritional status

The prevalence of underweight children (WAZ less than −2 SD) was 51.6% (49/95) at baseline (before initiation of ART), in which 28.4% (27/95) were severely underweight, and 23.2% (22/95) were moderately underweight. More females (30.5% [29/95]) were underweight than males (P=0.27). The prevalence of stunting (HAZ less than −2 SD was 49.1% (53/108) of the children, out of which 34.3% (37/108) were severely stunted. More females (28.7% [31/108]) were stunted than males (P=0.55). Approximately 31.5% of children (34/108) were wasted (BMI less than −2 SD), out of which 13.9% (15/108) were severely wasted. More females (20.4% [22/108]) were wasted than males (P=0.20) (Table 4).

Table 4 Prevalence of underweight children, stunting, and wasting before and 1 year after the initiation of ART among HIV-infected children attending ART clinics in Hiwot Fana Specialized University Hospital, Harar, eastern Ethiopa, 2011

Variables	Before ART, N (%)	One year after ART, N (%)	P-value
Weight-for-age Z score
Underweight	42 (53.2)	7 (8.9)	0.01*
Not underweight	37 (46.8)	72 (91.1)
Height-for-age Z score
Stunted	29 (46.0)	10 (15.9)	0.00
Not stunted	34 (54.0)	53 (84.1)
Body mass index
Wasted	21 (34.4)	6 (9.8)	0.41*
Not wasted	40 (65.6)	55 (90.2)

Note:

* Fisher’s exact test result.

Abbreviations: ART, antiretroviral therapy; HIV, human immunodeficiency virus; N, number of patients.

The prevalence of underweight children declined to 8.9% 1 year after initiation of ART. Exactly 6.3% of children (5/79) were severely underweight. In addition, approximately 15.9% (10/63) and 9.8% (6/48) of the children were stunted and wasted, respectively. The difference was statistically significant for underweight children and stunting (P<0.05) (Table 4).

Risk of anemia

In univariate analyses, the odds of being anemic were 4.25 times higher in preschool children compared to school children. Those variables with P-values less than 0.3, such as age, sex, and immunosuppression, were included for multivariate analysis. In this analysis, the odds of being anemic were 4.80 times higher in preschool children compared to school children. In addition, the odds of being anemic were 2.61 times higher in males compared to females (Table 5).

Table 5 Univariate and multivariate analysis for risk factors of anemia at baseline among HIV-infected children attending ART clinics in Hiwot Fana Specialized University Hospital, Harar, eastern Ethiopa, 2011

Variables	Crude odds ratio (95% confidence interval)	P-value	Adjusted odds ratio (95% confidence interval)	P-value
Age (years)
School (≥7)	1		1
Preschool (<7)	4.24 (1.85, 9.73)	0.00	4.80 (1.96, 11.75)	0.00
Sex
Female	1		1
Male	2.04 (0.92, 4.50)	0.08	2.61 (1.06, 6.45)	0.04
Immunosuppression
No evidence	1		1
Immunosuppressed	2.32 (0.63, 8.51)	0.21	2.0 (0.47, 8.51)	0.35

Abbreviations: ART, antiretroviral therapy; HIV, human immunodeficiency virus.

Discussion

Before initiation of ART in children, anemia in this study (54.4%) was almost as prevalent in the subjects as in a similar study done in Jimma, Ethiopia (53.1%),⁷ but it was less common than in reports from India (66%) and South Africa (73%).^12,13 Prevalence of anemia was higher among the preschool children in the current study. This finding was similar to findings in other similar studies.^4,12,13 This might be due to the increased growth requirements of preschool children, as described by Shet et al.¹² Anemia is caused by different ethological agents, which include nutrient deficiency, immunosuppression of erythropoiesis, drug side effects, opportunistic infections, HIV-associated malignancies, and other factors.^4,5,14 Anemia is also the major factor that reduces the survival odds of HIV-infected children.^2,3 Therefore, it is advisable to assess and tackle predisposing factors toward anemia in HIV-infected children.

There was a high prevalence of underweight, stunting, and wasting in children before the initiation of ART in this study. This is in agreement with some other reports.^14–16 This result might be due to direct consequences of the HIV infection, secondary clinical illnesses associated with HIV, a function of the child’s adverse environment, or a combination of these factors, as described by Shet et al.¹² The high prevalence of underweight, stunting, and wasting in children could not be correlated with magnitude of anemia in this study. However, it was correlated in one other study.¹²

The prevalence of anemia, underweight children, stunting, and wasting declined 1 year after the initiation of ART. The positive effects of using ART on the nutritional and anemic status of children has been published in other studies.^7,14,17–19 The mean increase in hemoglobin values after initiation of ART was statistically significant in both AZT- and d4T-based groups. This result is similar to a study from Jimma, Ethiopia.⁷ The mean increase in hemoglobin values in our study were 1 g/dL and 0.9 g/dL in the AZT- and d4T-based groups, respectively. This result is different when compared to 0.8 g/dL and 3.5 g/dL increases in AZT- and d4T-based groups in Jimma, Ethiopia,⁷ which may be because those children’s mean hemoglobin was lower before initiation of ART. The difference might also be due to differences in study design and sampling techniques.

Limitations of the current study

Those children with missing data were not included in this study, and this exclusion can be a potential limitation in a retrospective study. Thus, the true prevalence of anemia and malnutrition in our study area might not be reflected. In addition, the effect of opportunistic infections and nutritional support/supplementation on anemia, and the nutritional status of HIV-infected children were not assessed in this study.

Conclusion

In general, there was high prevalence of anemia and growth failure among the HIV-infected children in the Hiwot Fana Specialized University Hospital. They declined after initiation of ART, which highlights the importance of the treatment. Unfortunately, the problems in HIV-infected children still need attention. Therefore, as part of HIV-infected children’s care, health professionals should routinely diagnose hematological and nutritional parameters before and after the initiation of ART. Adherence counseling to strengthen the uptake of ART is also recommended. In addition, to plan appropriate interventions and understand the magnitude and the risk factors associated with anemia and nutritional status in HIV-infected children, further large-scale, prospective studies that include HIV-negative controls are recommended.

Author contributions

ZT and FM participated in proposal writing, data collection, analysis, interpretation, and critical revision of the manuscript. HM participated in data analysis, interpretation, and critical revision of the manuscript. All authors read and approved the final manuscript.

Acknowledgments

We acknowledge the Haramaya University Research and Publication Office for funding. Our thanks also extend to the Institutional Health Research and Ethics Review Committee of the Haramaya University for facilitating ethical clearance. We thank Muna Abdela for helping with data entry.

Disclosure

The authors report no conflicts of interest in this work.