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Original Research

Tuning the surface microstructure of titanate coatings on titanium implants for enhancing bioactivity of implants

, , , , &
Pages 3887-3896 | Published online: 08 Jun 2015

Abstract

Biological performance of artificial implant materials is closely related to their surface characteristics, such as microtopography, and composition. Therefore, convenient fabrication of artificial implant materials with a cell-friendly surface structure and suitable composition was of great significance for current tissue engineering. In this work, titanate materials with a nanotubular structure were successfully fabricated through a simple chemical treatment. Immersion test in a simulated body fluid and in vitro cell culture were used to evaluate the biological performance of the treated samples. The results demonstrate that the titanate layer with a nanotubular structure on Ti substrates can promote the apatite-inducing ability remarkably and greatly enhance cellular responses. This highlights the potential of such titanate biomaterials with the special nanoscale structure and effective surface composition for biomedical applications such as bone implants.

Introduction

In biomaterials science, the fabrication of bioscaffolds with both excellent biological performances and strong mechanical properties is a longstanding challenge. Especially, when implanted into hard tissue, like bone, the biomaterial should be strong enough to support considerable weight. Titanium and its alloys have been widely used for synthetic metallic implants in hard tissues due to their low toxicity, good biocompatbility, favorable mechanical properties, and great resistance to the corrosion compared to other metals.Citation1Citation4 However, although untreated implantable titanium with a smooth surface can support weight, it may lack the interspaces to accommodate tissue growth and may be unable to firmly bond with the bone and promote new bone formation on the surface, which may lead to the slow fixation of the implant and its gradual loosening over time. Hopefully, the surface modification of titanium and its alloys can optimize the biological performance of Ti-based biomaterials, and at the same time retain their favorable mechanical properties.Citation4Citation10 When implanted into a living body, the surface physicochemical properties of the biomaterials determine the interactions between them and the surrounding biological environment.Citation10Citation12 Recent efforts in this field have highlighted the importance of modifying the surface topography and composition of materials to achieve good biological performance of implants.Citation13Citation15 In thepast decade, design and fabrication of nanostructured titania and titanate layers on titanium have attracted much attention because of the improved biological performance of titanium and its widespread application in bone implants.Citation14Citation18 Generally, conventional titania and titanate nanostructures can be achieved by the sol–gel,Citation19,Citation20 sputtering,Citation21,Citation22 electrophoretic,Citation23,Citation24 chemical vapor deposition,Citation25 anodization,Citation26,Citation27 or hydrothermal methods.Citation28Citation31 Among these techniques, hydrothermal method is one of the most facile techniques due to its simplicity, cost-effectiveness, and suitability for mass production. Wu and coworkers reported obtaining H2Ti3O7 powders with nanotubes, nanofibers, nanowires, and nanobelt structures by the alkali hydrothermal method.Citation29 Wang also reported that titanate nanobelt powders with tunable wettability provide the possibility for chemical sensors, which were prepared using a modified hydrothermal method.Citation27 The nanostructure of titania and titanate could be effectively controlled by various parameters of the hydrothermal method. Recently, some groups have reported the fabrication of titania and titanate layers on titanium substrates for biomedical applications.Citation32 However, reports on surface topography affecting the interaction between bone and the implant and further deciding the preloading integration success rate of implants are still rare.

Earlier studies have shown that, when artificial materials were implanted into bone defects, they were generally encapsulated by the fibrous tissue, leading to their isolation from the surrounding bone, and did not integrate with the bone. This has been a critical problem in their applications to bone repair. In order to overcome this problem, bioactive materials that can spontaneously bond to the living bone without the formation of surrounding fibrous tissue have been studied widely. Bioactivity of the implant materials means that a layer of apatite, which usually consists of nanosized crystals of carbonate-ion-containing apatite, is induced to form on the surface of the implant materials. Due to its similarity in composition with the mineral phase in bone, this apatite layer can be recognized by bone tissue, and therefore promotes integration with bone.Citation33Citation36 Previous studies have shown that simulated body fluid (SBF) has ion concentrations nearly equal to that of the human blood plasma and can well reproduce the in vivo apatite formation.Citation36,Citation37 Thus, the most efficient method for investigating the biological behavior of the biomaterials and the biomimetic deposition of the apatite layer is to immerse the samples into SBF. For bone implant, it is very important to find out the relationship between the surface characteristics of materials and the bioactivity of materials as well as the synergistic effect between different surface characteristics in enhancing apatite-forming ability. In this work, we developed a hydrothermal method to tune the morphologies of titanate layers on titanium surfaces. Their surface morphology and chemical composition were characterized using several techniques used in materials science. To show that the surface structures prepared by the hydrothermal method are suitable for use as bone implants, the effect of the morphologies of titanate layers on their ability to promote apatite formation was investigated. In vitro cell culture was also performed to demonstrate the materials’ biocompatibility and to explore the biomedical applications of the prepared biomaterials.

Materials and methods

Preparation of samples

Commercially pure titanium foils (99.7%, Aldrich) with the dimension of 10 mm ×10 mm ×2 mm were employed as substrates. They were immersed in an etchant containing 10% (v/v) HNO3, 1% (v/v) HF, and deionized (DI) water for 1 minute. Finally, the Ti samples were cleaned consecutively with acetone, ethyl alcohol, and DI water, each for 15 minutes under ultrasonication. The Ti foils were then placed in a Teflon-lined stainless steel vessel containing 10 M NaOH solution, the vessel was sealed and hydrothermally treated at 100°C and 140°C for 2 hours and at 150°C for 3 hours, and cooled naturally in air. To obtain a fixed film, the treated Ti substrates were initially rinsed with 5 M NaOH, and the NaOH solution was gradually diluted with DI water to pH 7. The obtained samples were directly dried in air, and are referred to as Ti100, Ti140, and Ti150, respectively.

Immersion test in SBF

SBF was used as an incubation solution for apatite formation on the prepared coatings. The ion concentrations of SBF were nearly equal to that of human blood plasma. SBF was prepared according to Kokubo’s formulation by dissolving reagent-grade mixtures of NaCl, NaHCO3, KCl, K2HPO4·3H2O, MgCl2·6H2O, CaCl2, and Na2SO4 in DI water and buffering at pH 7.40 with tris-hydroxymethyl aminomethane and 1.0 M hydrochloric acid (HCl) at 36.5°C.Citation36 All the prepared samples were soaked in SBF at a constant temperature of 36.5°C. As controls, untreated Ti plates were also soaked in SBF under the same conditions. All the samples were kept in the vertical position inside polypropylene tubes (one per tube). After soaking in SBF for different periods, ie, 7 days and 14 days, the samples were gently washed with DI water and dried at room temperature.

Characterization

The surface morphologies of all the samples were observed by scanning electron microscopy (SEM, Hitachi-S4800, Hitachi Ltd., Tokyo,, Japan) at 20 kV, and elemental analysis was carried out by an X-ray energy dispersion spectroscopy (EDS) attachment that was directly connected to the SEM. The corresponding crystal structural characterization of all the samples was done using their X-ray diffraction (XRD, PANalytical X’pert PRO, PANalytical Ltd., Almelo, the Netherlands) patterns with a Cu Kα radiation source at 40 kV and 30 mA. The XRD patterns were collected over the 2θ range 20°–60° using a step size of 0.0167 and a time per step of 20. Fourier transform infrared spectroscopy (FTIR, Nicolet-740) was employed in transmission mode using the KBr technique to analyze the chemical composition of the coatings before and after soaking in SBF. The Raman measurements at room temperature were performed using a Raman spectrometer (Renishaw, UV-vis Raman System 1000) equipped with an Ar+ laser (λ =514.5 nm). The structure of the formed nanotubes was characterized using transmission electron microscopy (TEM, Philips-FEI, Tecnai F30, the Netherlands). The nanotubes were scraped off from the Ti substrate, dispersed in ethanol, and deposited on a copper grid for TEM analysis. The water contact angles were measured at ambient temperature using a contact angle instrument (OCA 40, Data Physics, Corporation, Filderstadt, Germany) with a drop volume of 5 μL. DI water (Millipore, 18 MΩ·cm) was employed as the medium for the contact angle measurements. All reported contact angles are the average of measurements on four drops at different locations of the sample surface.

In vitro cell culture

Preosteoblast cells MC3T3-E1 were expanded in alpha minimum essential medium (Thermo Fisher Scientific, Waltham, MA, USA) containing 10% fetal bovine serum (Life Technologies, Carlsbad, CA, USA) and 1% penicillin/streptomycin (Life Technologies, Carlsbad, CA, USA). Cells were incubated at 37°C in a humidified atmosphere with 5% CO2, and the medium was replaced every 48 hours. After culturing in the growth medium till 80%–90% confluence, they were harvested to seed onto the coated samples.

Sterilized samples (10 mm ×10 mm ×2 mm) were transferred into 24-well plastic culture plates. MC3T3-E1 cells were seeded onto the prepared samples at a density of 1×105 cells per sample. Cells were incubated in the culture medium (1 mL/well) at 37°C under 5% CO2 atmosphere for 1 day, 3 days, and 7 days, with the medium replaced every 3 days until being harvested.

Cell viability and proliferation were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT; Sigma) staining. At desired time points, the cell-seeded samples were incubated in MTT solution (5 mg/mL MTT in cell culture medium) in a 5% CO2 incubator at 37°C for 4 hours. The intense purple formazan derivative formed via cell metabolism was eluted and dissolved in dimethylsulfoxide (DMSO) with the dosage of 400 μL per well. The absorbance was measured at 570 nm with a reference wavelength of 690 nm using a microplate reader (BioTek Synergy4). The cell number was correlated to the optical density.

Fluorescein diacetate (FDA) molecular probe was used as a viable cell marker to investigate the growth of cells on the prepared coatings. After 3 days of culture, the cell-seeded samples were washed with PBS, incubated with 30 μg/mL FDA solution for 15 minutes, and washed with PBS. The positive cells were observed immediately under a laser confocal microscope (Olympus FluoView 1000).

Results and discussion

Micromorphology and compositions of the Ti foil after hydrothermal treatment

Typical SEM micrographs of the Ti plates before and after hydrothermal treatment at 100°C–150°C are shown in . After hydrothermal treatment for 2 hours at 100°C, a porous network structure with the pore size of 200−300 nm in diameter was observed on the surface of Ti100 (). shows that, once the temperature was increased to 140°C, bumped crystals sheets were formed on the surface of Ti140. After hydrothermal reaction at 150°C for 3 hours (Ti150), a porous structure consisting of tiny fiber-like crystals with an average diameter approximately 20 nm was obtained (). The TEM image in shows that the fiber-like crystals have typical nanotubular structure, which are made of multilayered sheets. From the above observation, it indicates that the surface microtopography of Ti substrate can be controlled effectively by varying the hydrothermal reaction temperature and time. The EDS spectrum shown in identifies that the elemental composition of prepared samples under the different hydrothermal conditions consists mainly of oxygen, titanium, and sodium.

Figure 1 SEM micrographs, TEM images and EDS spectrum of Ti plates with hydrothermal treatment at different temperatures.

Notes: SEM micrographs (A) Untreated Ti. (B) Ti100. (C) Ti140. (D) Ti150. (E) TEM images of the nanotubes obtained from hydrothermal treatment at 150°C for 3 hours (Ti150). (F) EDS spectrum of the hydrothermally treated samples.

Abbreviations: SEM, scanning electron microscopy; TEM, transmission electron microscopy; EDS, energy dispersion spectroscopy.

Figure 1 SEM micrographs, TEM images and EDS spectrum of Ti plates with hydrothermal treatment at different temperatures.Notes: SEM micrographs (A) Untreated Ti. (B) Ti100. (C) Ti140. (D) Ti150. (E) TEM images of the nanotubes obtained from hydrothermal treatment at 150°C for 3 hours (Ti150). (F) EDS spectrum of the hydrothermally treated samples.Abbreviations: SEM, scanning electron microscopy; TEM, transmission electron microscopy; EDS, energy dispersion spectroscopy.

The crystalline structures of the prepared coatings were identified by XRD, as shown in . The characteristic diffraction peaks of the samples at 24°, 28°, and 48° are assigned to the crystal of sodium titanate (Na2Ti3O7)Citation26, as shown by the curves b–d in . The corresponding peaks in curves b and c are extremely inconspicuous because the sodium titanate layers on Ti100 and Ti140 are too thin to be detected by XRD. The intensities of these characteristic peaks in curve d increase clearly because of the increased thickness of this sodium titanate layer by raising the reaction temperature and time. The above change is also confirmed by the Raman spectra in . With increasing reaction temperature and time, the Raman spectra of hydrothermally treated samples show more obvious bands near 165 cm−1, 278 cm−1, 445 cm−1, 696 cm−1, and 900 cm−1, which are characteristic of Na2Ti3O7.Citation38

Figure 2 XRD spectra of samples with hydrothermal treatment at different temperatures.

Notes: (a) Untreated Ti. (b) Ti100. (c) Ti140. (d) Ti150.

Abbreviations: N, Na2Ti3O7; XRD, X-ray diffraction.

Figure 2 XRD spectra of samples with hydrothermal treatment at different temperatures.Notes: (a) Untreated Ti. (b) Ti100. (c) Ti140. (d) Ti150.Abbreviations: N, Na2Ti3O7; XRD, X-ray diffraction.

Figure 3 Raman spectra of the samples with hydrothermal treatment at different temperatures.

Notes: (a) Untreated Ti. (b) Ti100. (c) Ti140. (d) Ti150.

Figure 3 Raman spectra of the samples with hydrothermal treatment at different temperatures.Notes: (a) Untreated Ti. (b) Ti100. (c) Ti140. (d) Ti150.

Wettability of the prepared samples

Images of water droplets lying on flat prepared samples surfaces and the corresponding contact angle values are shown in . The water contact angle for the untreated Ti plates is approximately 58.4°±1.1°. After the hydrothermal treatment, the water droplet quickly spread and wetted all the treated samples (Ti100, Ti140, and Ti150), and the water contact angles decreased to near 0°, indicating that all the treated samples were superhydrophilic.

Figure 4 Water contact angles of the samples with hydrothermal treatment at different temperatures.

Notes: (a) Untreated Ti. (b) Ti100. (c) Ti140. (d) Ti150.

Figure 4 Water contact angles of the samples with hydrothermal treatment at different temperatures.Notes: (a) Untreated Ti. (b) Ti100. (c) Ti140. (d) Ti150.

Biological performance of prepared samples

Bone-like apatite-forming behavior

To evaluate the bioactivity of the samples prepared by hydrothermal treatment, the formation behavior of the apatite layer on the prepared samples was examined by immersing them in SBF for up to 14 days. After incubation in SBF for 7 days and 14 days, the surface morphology of the samples with or without hydrothermal treatment was observed by SEM, as shown in . Among all the samples, there were no new layers formed on the surfaces of Ti100, Ti140, Ti150, and untreated Ti foil after soaking in SBF for 7 days. When the immersion time was increased to 14 days, the entire surface of the Ti150 sample was covered by newly formed layers (). The newly formed layers exhibited a network structure consisting of nanosheets. Unfortunately, there was still no apatite layer formation on the surface of Ti100, Ti140, and untreated Ti for the same soaking time (). The elemental composition of the newly formed layers consisted mainly of Ca, P, Ti, O, and a small fraction of magnesium, and the average Ca/P molar ratio was approximately 1.41, as evaluated from the EDS spectra of . The FTIR spectrum of the layer newly formed on the samples surface is shown in . The peak at 1,035 cm−1 is assigned to the P–O asymmetric stretching mode (υ3) of the PO43− group in apatite.Citation39 The bending modes of the phosphate O–P–O bonds are detected at 604 cm−1 and 565 cm−1.Citation40 The peaks corresponding to the CO32− vibrational mode are found at 874 cm−1, 1,421 cm−1, and 1,460 cm−1, which means that the PO43− sites of the apatite structure are partially substituted by CO32− groups (B-type substitution).Citation37 Based on the EDS and FTIR results, it can be concluded that newly formed layer is calcium-deficient carbonated apatite with traces of magnesium, which has similar mineral composition as that of natural bones.

Figure 5 SEM micrographs of the various samples after soaking in SBF for 7 days.

Notes: (A) Untreated Ti. (B) Ti100. (C) Ti140. (D) Ti150.

Abbreviations: SEM, scanning electron microscopy; SBF, simulated body fluid.

Figure 5 SEM micrographs of the various samples after soaking in SBF for 7 days.Notes: (A) Untreated Ti. (B) Ti100. (C) Ti140. (D) Ti150.Abbreviations: SEM, scanning electron microscopy; SBF, simulated body fluid.

Figure 6 SEM micrographs of the various samples after soaking in SBF for 14 days.

Notes: (A) Untreated Ti. (B) Ti100. (C) Ti140. (D) Ti150.

Abbreviations: SEM, scanning electron microscopy; SBF, simulated body fluid.

Figure 6 SEM micrographs of the various samples after soaking in SBF for 14 days.Notes: (A) Untreated Ti. (B) Ti100. (C) Ti140. (D) Ti150.Abbreviations: SEM, scanning electron microscopy; SBF, simulated body fluid.

Figure 7 EDS spectrum (A) and FTIR spectrum (B) of the newly formed layer on the Ti150 after soaking for 14 days.

Abbreviations: EDS, energy dispersion spectroscopy; FTIR, Fourier transform infrared spectroscopy.

Figure 7 EDS spectrum (A) and FTIR spectrum (B) of the newly formed layer on the Ti150 after soaking for 14 days.Abbreviations: EDS, energy dispersion spectroscopy; FTIR, Fourier transform infrared spectroscopy.

Furthermore, apatite formation can be identified from the Raman spectra since the intensity of the characteristic peak at 960 cm−1 originating from the υ1 symmetric stretching of the P–O mode is appreciable.Citation39 It failed to show the formation of the apatite layers on the surfaces of all samples after the immersion in SBF for 7 days (). After soaking in SBF for 14 days, the Raman peak at 960 cm−1, which is assigned to the apatite phase, suggests that the deposited layer on the surface of Ti150 possesses a crystalline structure (), which can be verified by the SEM images in . It is noteworthy that only the hydrothermally treated sample with a nanotubular structure could induce apatite deposition with continued soaking in SBF for 14 days.

Figure 8 Raman spectra of the various samples after soaking in SBF for (A) 7 days and (B) 14 days.

Notes: (a) Untreated Ti. (b) Ti100. (c) Ti140. (d) Ti150.

Abbreviation: SBF, simulated body fluid.

Figure 8 Raman spectra of the various samples after soaking in SBF for (A) 7 days and (B) 14 days.Notes: (a) Untreated Ti. (b) Ti100. (c) Ti140. (d) Ti150.Abbreviation: SBF, simulated body fluid.

Cell proliferation and morphology of the prepared coatings

MTT assay was used to compare the cell viability of the prepared samples (). In order to investigate the cell response to the surface characteristics of the prepared samples, the adhesion and proliferation of preosteoblast MC3T3 cells were measured. There was a clear temporal proliferation profile after 1 day, 3 days, and 7 days of culture on all the samples. As shown in , the viability of preosteoblast MC3T3 cells cultured on the Ti100, Ti140, and Ti150 samples appeared significantly higher than that of the untreated Ti at 7-day time points (P<0.05). In addition, sample Ti150 showed the highest proliferation at 7-day time points, showing a viability ~1.4 times higher than in the untreated Ti. These results clearly indicate that Ti150 with a nanotubular structure may promote cell proliferation, allowing more cells to grow in a congenial environment.

Figure 9 Preosteoblast MC3T3 cell proliferation on untreated Ti, Ti100, Ti140, and Ti150 for 1 day, 3 days, and 7 days.

Figure 9 Preosteoblast MC3T3 cell proliferation on untreated Ti, Ti100, Ti140, and Ti150 for 1 day, 3 days, and 7 days.

Laser confocal microscopy was used to study the dynamics of cell adhesion and spreading on the prepared samples after fluorescent straining with FDA, as shown in . MC3T3 cells on untreated Ti show an uneven morphology after 3 days in culture. Compared to the cells adhering to untreated Ti, MC3T3 cells adhered to and spread on the treated samples more uniformly. As shown in , a larger number of MC3T3 cells got attached to and fully covered the surface of sample Ti150.

Figure 10 Laser confocal micrographs of preosteoblast MC3T3 cells incubated on untreated Ti, Ti100, Ti140, and Ti150 stained with FDA molecular probe for 3 days.

Abbreviation: FDA, fluorescein diacetate.

Figure 10 Laser confocal micrographs of preosteoblast MC3T3 cells incubated on untreated Ti, Ti100, Ti140, and Ti150 stained with FDA molecular probe for 3 days.Abbreviation: FDA, fluorescein diacetate.

In general, the biological performance of biomaterials is attributed to their surface structure and composition. Bone-like apatite formation on the surface of biomaterials is the essential requirement for an artificial material to integrate with the surrounding bonetissue.Citation35,Citation36 Some previous studies have suggested the catalytic effect of Ti–OH groups for apatite nucleation, and these functional groups are abundant on the surface of sodium titanate or titania.Citation16,Citation41 In our experiments, the XRD and Raman results ( and ) show that sodium titanate (Na2Ti3O7) layers are formed on the Ti substrate after hydrothermal treatment in a 10 M NaOH solution. In other words, all the treated samples had the same surface composition. Interestingly, only the hydrothermally treated samples with a unique nanotubular structure (Ti150) could induce apatite formation after being soaked in SBF for 14 days, while no apatite layers were observed on the surfaces of the samples without the nanotubular structure (untreated Ti, Ti100, and Ti140) after immersion for the same time. It indicates that the formed nanotubular structure could improve the apatite-forming ability effectively. Our results show that no nanotubular structure is obtained after hydrothermal treatment at 100°C and 140°C; however, such structures are observed at 150°C (). It indicates that temperature can accelerate the formation of the nanotubular structure. These observations show that temperature is the most important factor that affects the surface microtopography of the Ti plate during hydrothermal treatment. It can be concluded from these findings that the surface microtopography of the materials, which has a significant impact on the formation of the apatite, can be well controlled by the reaction temperature in hydrothermal treatment. Obviously, the samples Ti150 with the nanotubular structure possess a large effective surface area. Meanwhile, when the treated samples are soaked in SBF, Na+ ions released from the sodium titanate layer exchange with H3O+ ions to form Ti–OH groups. It can be inferred that the large surface area of Ti150 induces more Ti–OH groups to form on the Ti surface. The large number of Ti–OH groups formed immediately reacts with the Ca2+ ions in SBF to accelerate the formation of amorphous calcium titanate. This calcium titanate later combines with the phosphate ions in SBF to form amorphous calcium phosphate. Once the apatite nuclei are formed, they can grow spontaneously by consuming the surrounding calcium and phosphate ions due to the high supersaturation in SBF, and finally transform into bone-like apatite with a small concentration of Mg and Na, which is the most stable phase in the body fluid, as shown in .

All experiments of cell culture have demonstrated that the treated sample could improve MC3T3 cell adhesion, spreading, and proliferation. It is noted that after hydrothermal treatment, although superhydrophilic titanate surfaces are formed on all the treated samples (Ti100, Ti140, and Ti150), Ti150 with the nanotubular surface structure showed better cell response, indicating excellent biocompatibility of the nanotubular structure. When the nanotubular structure is formed, the surface area of Ti150 increases distinctly. It could provide sufficient space for cell anchoring, spreading, and proliferation. In the meantime, it has been proposed that cell filopodia can recognize the nanoscale topography.Citation14 The nanotubular structure formed on Ti150 presents a very high affinity to the cells incubated on it.

Based on the results of SBF immersion test and cell culture test in this study, it is clear that the nanotubular structure plays a very important role in enhancing the apatite-forming ability and cell response. These findings clearly demonstrate that the prepared Ti150 samples have excellent biocompatibility and high bioactivity, which are attributed to the special nanoscale structure and a suitable surface composition.

Conclusion

Bioactive materials have been known to spontaneously integrate with living bones by promptly forming bone-like apatite on their surfaces, which is important for fast bone regeneration. In this study, chemical treatment with high concentrations of an alkaline solution at high temperature was employed to rapidly modify the surface morphology and composition of titanium materials to enhance their biological performance. The samples with different surface characteristics were soaked in SBF solution and seeded by preosteoblast MC3T3 cells for various times to investigate their biological performance. Experimental results indicated that, compared with the traditional smooth, untreated Ti implants, titanate materials with the nanotubular structure had a great promoting effect on the apatite-forming ability and improved preosteoblast MC3T3 cell response due to their special nanoscale structure and suitable surface composition. This relatively simple methodology and the outstanding performance of the nanostructured titanate film would be convenient for future bone and hard-tissue engineering applications.

Acknowledgments

The authors are grateful for the support from the National Natural Science Foundation of China under Grants 51203108, 51073113, 91027039, 51373110 and 21321062, the National Scientific Support Program of China under Grants 2012BAI07B09, the Natural Science Foundation of Jiangsu Province of China under Grants BK2011355 and BK20130313, and the Natural Science Foundation of the Jiangsu Higher Education Institutions of China under Grants 11KJB430011 and 10KJA540046.

The authors also acknowledge support from the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Qing Lan Project for Excellent Scientific and Technological Innovation Team of Jiangsu Province (2012), and Project for Jiangsu Scientific and Technological Innovation Team (2013).

Disclosure

The authors report no conflicts of interest in this work.

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