https://orcid.org/0000-0003-0275-6269
Dear editor
We are grateful to the authors for publishing their research “Antipyretic Potential of 80% Methanol Extract and Solvent Fractions of Bersama abyssinica Fresen. (Melianthaceae) Leaves Against Yeast-Induced Pyrexia in Mice” in the Journal of Experimental Pharmacology 2023:15 81–91.Citation1 Understanding the efficacy of B. abyssinica’s leaf extract and solvent fractions in vivo requires an understanding of this information. We would to communicate our thoughts on the test of preparation and extraction of plant material method used against yeast-induced pyrexia in mice in this study.
In this study, pyrexia or fever was induced by injecting a suspension of 30% w/v yeast extract powder, at a dose of 3 g/kg or 10 mL/kg, in 0.9% normal saline beneath the nape of the neck. After 18 hours, the rectal temperature was recorded. The temperature was recorded at 0.5, 1, 1.5, 2, 2.5, and 3 hours after dosing with crude extract and solvent fractions. The previous experiment had similar methods.Citation2 In another experiment, the temperature was recorded at 30 minutes, 1 hour, 2 hours, 3 hours, and 24 hours after treatment.Citation3
Preparing medicinal plants for experimentation is the first and most important stage in producing high-quality research results. Before beginning the process, it involves extracting and determining the quantity and quality of bioactive components.Citation4 The next thing that needs to be done according to WHO standards for herbal medicines is potentially hazardous contaminants and residues. There are potential sources for pollutants and residues, as well as the stages of production where they might be visible. Some of them are regarded as inevitable contaminants or herbal medicinal residues. Physical, chemical, and biological pollutants are the two categories of contaminants found in herbal medicines. There could be significant residues of several agrochemicals and organic solvents in herbal medications.Citation5
Because of this, the test is potentially hazardous contaminants and residues on herbal medicine may also be evaluated to be used in the near future to complete methods in preparation of medicinal plants. In general, this study still bears faithfully to the initial principle of “Extraction of Plant Material” that will be used as an antipyretic in mice in vivo and may be further explored and evaluated to address the suggestions stated above.
Disclosure
The authors report no conflicts of interest in this communication.
Acknowledgments
It is appropriate to express appreciation to Bantayehu Addis Tegegne and Agumas Alemu Alehegn for their good teamwork in this study. All authors would also like to thank Dr. Sunarno and all researchers in the Center for Biomedical Research, National Research and Innovation Agency (BRIN) for their ongoing assistance.
References
- Tegegne BA, Alehegn AA. Antipyretic potential of 80% methanol extract and solvent fractions of bersama abyssinica fresen. (Melianthaceae) leaves against yeast-induced pyrexia in mice. J Exp Pharmacol. 2023;15:81–91. doi:10.2147/JEP.S390825
- Yimer T, Emiru YK, Kifle ZD, Ewunetei A, Adugna M, Birru EM. Pharmacological evaluation of antipyretic and antioxidant activities of 80% methanol root extract and derived solvent fraction of echinops kebericho M. (Asteraceae) in mice model. Biomed Res Int. 2021;2021:1–8. doi:10.1155/2021/6670984
- Reza MS, Jashimuddin M, Ahmed J, et al. Pharmacological investigation of analgesic and antipyretic activities of methanol extract of the whole part of Aeginetia indica. J Ethnopharmacol. 2021;271:113915. doi:10.1016/j.jep.2021.113915
- Abubakar A, Haque M. Preparation of medicinal plants: basic extraction and fractionation procedures for experimental purposes. J Pharm Bioallied Sci. 2020;12:1–10. doi:10.4103/jpbs.JPBS_175_19
- World Health Organization. Guidelines for Assessing Quality of Herbal Medicines with Reference to Contaminants and Residues. World Health Organization; 2007.