Efficacy and visual prognostic factors of intravitreal bevacizumab as needed for macular edema secondary to central retinal vein occlusion

Abstract

Purpose

Our aim was to investigate the efficacy and prognostic factors of intraocular injections of bevacizumab as needed in patients with macular edema secondary to central retinal vein occlusion (CRVO).

Methods

This is a retrospective study including 28 eyes of 27 consecutive patients with macular edema due to CRVO and followed for at least 6 months. The mean age of the patients was 66.3 years. The patients underwent an intravitreal injection of bevacizumab (1.25 mg) at the initial visit. Retreatments were performed when macular edema was persistent or worsened (as-needed regimen). The primary outcome measure was the mean change in best-corrected visual acuity (BCVA). The change in central retinal thickness (CRT) was evaluated as the secondary outcome. Finally, the factors useful for predicting BCVA outcome were determined.

Results

The mean number of injections was 1.8 over a period of 6 months. The mean BCVA (logarithm of minimum angle of resolution) was significantly improved at 1 (−0.097), 3 (−0.14), and 6 months (−0.25) after the initial injection (P<0.05, <0.01, and <0.001, respectively). The mean CRT was also improved significantly at 1 (−250.4), 3 (−150.0), and 6 months (−187.2) (P<0.001 each). Earlier treatment and better improvement in BCVA at 1 month after the initial treatment were the prognostic factors significantly associated with better visual outcomes at 6 months (P=0.047 and 0.029, respectively).

Conclusion

Intravitreal injection of bevacizumab as needed significantly improved visual acuity and macular edema in CRVO patients. Time before the treatment and early response to the treatment were important factors for the visual outcome.

Keywords:

• central retinal vein occlusion
• bevacizumab
• macular edema
• efficacy
• prognostic factor
• pro re nata regimen

Introduction

Central retinal vein occlusion (CRVO) is a common vision-threatening disorder that causes sudden and severe vision loss in adults. Macular edema is one of the main causes of visual impairment in cases of CRVO.^1–5 As with other retinal vascular diseases, CRVO is associated with a marked increase of intraocular vascular endothelial growth factor (VEGF).^6–8 Recent randomized controlled trials demonstrated that intravitreal injections of ranibizumab or aflibercept were effective in reducing macular edema and improving the vision in patients with CRVO.^9–11 However, these studies required frequent injections (6 monthly injections) to obtain the mean best-corrected visual acuity (BCVA) improved in two to three lines with early treatment diabetic retinopathy study chart at 6 months, which could be very expensive treatments. Alternatively, several studies have reported that off-label use of bevacizumab, a recombinant humanized monoclonal antibody, against all isoforms of VEGF-A, was effective in reducing macular edema in patients with CRVO.^12–18 Because bevacizumab is much cheaper than ranibizumab or aflibercept, this drug class may have higher cost-effectiveness in treating CRVO in current clinical practice. Moreover, injections with a pro re nata (PRN) (as-needed) regimen may reduce the total number of injections compared to scheduled injections. This study was conducted to evaluate the efficacy of intravitreal injections of bevacizumab (IVB) as needed over 6 months in patients with macular edema secondary to CRVO, and we performed multivariate analysis to determine the factors associated with patients’ vision after treatment.

Methods

This is a retrospective study of 28 eyes from 27 consecutive patients with CRVO-related macular edema who were treated with IVB and followed up for at least 6 months. Ten patients received other treatments before the initial IVB (sub-Tenon injection of triamcinolone acetonide for four eyes, intravitreal injection of tissue plasminogen activator for four eyes, intravenous injection of urokinase for three cases, photocoagulation for one eye, and vitrectomy for one eye).

All patients underwent full ophthalmological examinations, including fluorescein angiography (FA) and optical coherent tomography (OCT), at presentation. Four patients were diagnosed as ischemic type and the others were considered the nonischemic type. Treatment consisted of the first bevacizumab injection (1.25 mg) at the initial visit, and follow-up visits were scheduled every 4 weeks thereafter. Additional injections were given when persistent or recurrent edema was detected by OCT (PRN protocol).

The demographic and clinical data analyzed were patient age and sex, systemic diseases including hypertension and diabetes mellitus, BCVA, and central retinal thickness (CRT) at presentation and at the 1-, 3-, and 6-month follow-up. The BCVA values were determined using a Landolt C chart and were converted to logarithm of minimum angle of resolution (logMAR) for calculation and description. The instrument used for CRT measurements was Cirrus HD-OCT model 4000 (Carl Zeiss Meditec, Dublin, CA, USA). The number of bevacizumab injections and adverse events were recorded as well. To determine the factors useful for predicting the resolution of the lesion, multivariable logistic regression analysis using a stepwise method was performed using the variables that showed some trend toward significant association (P-value <0.2) in the univariate logistic model.

This study was approved by the Institutional Review Board at the Kobe University Graduate School of Medicine and was conducted in accordance with the Declaration of Helsinki. Written informed consent was obtained from all subjects.

Statistical analysis

All statistical analyses were performed by MedCalc version 113 software (MedCalc Software, Ostend, Belgium). Paired t-test, Wilcoxon signed rank test – whichever the most appropriate – was applied to compare any two groups. P-values of 0.05 or less were considered statistically significant.

Results

The summary of the clinical data of the participants is shown in Table 1.

Table 1 Summary of the patients

Number of eyes (cases, N)	28 (27)
Right eye (n) %	20 (71.4%)
Males (n) %	16 (59.2%)
Mean age (years) (±SD)	66.3 (±13.5)
Duration from the onset to IVB (months)	4.6 (±5.8) (range: 0.5–27)
Baseline BCVA (logMAR) (±SD)	0.71 (±0.44)
Baseline CRT (μm) (±SD)	603 (±183)
Previous treatment (n) (%)	10 (35.7%)
Duration from previous treatment to IVB (months)	5.3 (±4.2) (range: 2–15)
Ischemic type (NPA $10 disk area) (%)	4 (14.3%)
Inner retinal fluid (n) (%)	22 (78.6%)
Outer retinal fluid (n) (%)	24 (85.7%)
Subretinal fluid (n) (%)	13 (46.4%)
Hypertension (n) (%)	18 (64.3%)
Diabetes mellitus (n) (%)	6 (21.4%)

Abbreviations: IVB, intravitreal bevacizumab; BCVA, best-corrected visual acuity; CRT, central retinal thickness; logMAR, logarithm of minimum angle of resolution; NPA, nonperfusion area; SD, standard deviation.

The mean duration between the onset of CRVO and the first treatment was 4.6±5.8 months (range: 0.5–27 months). The majority of patients had hypertension (n=18, 64.3%), followed by diabetes mellitus (n=6, 21.4%). No patient in this study had diabetic retinopathy. The mean number of IVB injections was 1.8±0.9 (range: 1–4) over the 6 months of follow-up. Sixteen eyes (57.1%) showed the recurrence of macular edema during the 6 months and the mean recurrence-free period was 2.6±0.9 months (range: 1–4 months).

In the time-course analysis, the mean BVCA after the initial IVB was significantly improved at 1, 3, and 6 months of follow-up as compared to baseline (−0.097, −0.14, and −0.25 logMAR units; P<0.05, <0.01, and <0.001, respectively; Wilcoxon signed rank test) (Figure 1 and Table 2). In the time course of the mean CRT over 6 months, significant improvement was observed at 1, 3, and 6 months of follow-up compared to baseline (−250.4 μm, −150.0 μm, and −187.2 μm, P<0.001 each; Wilcoxon signed rank test) (Figure 1 and Table 2). The proportions of eyes in which the BCVA improved more than 0.3 logMAR, changed within 0.3 logMAR, and deteriorated more than 0.3 logMAR from baseline to 1, 3, and 6 months posttreatment are shown in Figure 2, which suggested an increase of eyes with better outcome over time after the initial treatment.

Figure 1 Time course of the mean BCVA and CRT from baseline in CRVO patients with IVB treatments.

Notes: Time course of (A) BCVA (logMAR) and (B) CRT.
Abbreviations: BCVA, best-corrected visual acuity; CRT, central retinal thickness; CRVO, central retinal vein occlusion; IVB, intravitreal bevacizumab; logMAR, logarithm of minimum angle of resolution.

Figure 2 Chronological alterations in the distribution of visual acuity changes in CRVO patients with IVB treatments.

Notes: The proportions of eyes in which BCVA improved more than 0.3 logMAR, changed within 0.3 logMAR, and deteriorated more than 0.3 logMAR from baseline to 1, 3, and 6 months posttreatment. The number of eyes is indicated in each bar.
Abbreviations: BCVA, best-corrected visual acuity; CRVO, central retinal vein occlusion; IVB, intravitreal bevacizumab; logMAR, logarithm of minimum angle of resolution.

Table 2 Changes in BCVA and CRT from baseline with IVB treatment

	1 month	P*	3 months	P*	6 months	P*
Change in BCVA	−0.097±0.24	0.011	−0.14±0.28	0.0049	−0.25±0.25	0.0001
Change in CRT	−250.4±171.3	<0.0001	−150.0±208.1	0.0005	−187.2±198.4	0.0002

Note:

* Wilcoxon signed rank test (comparison with baseline).

Abbreviations: BCVA, best-corrected visual acuity (logMAR); CRT, central retinal thickness; IVB, intravitreal bevacizumab; logMAR, logarithm of minimum angle of resolution.

The results of univariate logistic regression analyses are shown in Table 3. Age, sex, pretreatment BCVA, duration from the onset to the treatment, existence of previous treatment, ischemic change, the status of macular edema, systemic hypertension, and diabetes mellitus did not show a trend toward significant association. In the multivariate logistic regression analysis, duration from the onset of disease to the initial treatment was an independent prognostic factor significantly associated with a good visual acuity (>20/30) at 6 months (odds ratio: 0.62; 95% confidence interval: 0.38–0.99; P=0.047). In addition, multiple linear regression analysis revealed that the change in visual acuity at 1 month after the initial IVB was an independent prognostic factor significantly associated with the change in visual acuity at 6 months (r²=0.18, P=0.029).

Table 3 Factors that showed some trend toward significant association (P-value <0.2) in the univariate logistic model

Factors	OR	95% CI	P
Duration from onset to treatment (months)	0.62	0.38–0.99	0.047
Baseline BCVA (logMAR)	0.08	0.004–1.5	0.10
Presence of prior treatment (yes =1, no =0)	4.0	0.68–23.4	0.12
Systemic hypertension (yes =1, no =0)	5.7	0.59–55.6	0.13
Pretreatment macular edema (yes =1, no =0)	0.29	0.045–1.9	0.20

Abbreviations: OR, odds ratio; CI, confidence interval; BCVA, best-corrected visual acuity; logMAR, logarithm of minimum angle of resolution.

No ocular or systemic complication was detected during the follow-up period in the current study.

Discussion

The current study has shown that IVB with a PRN regimen improved patients’ vision affected by macular edema secondary to CRVO. Duration from the onset of disease to the initial treatment and the change in visual acuity at 1 month after initial treatment were independent prognostic factors significantly associated with the visual acuity at 6 months of follow-up.

Intravitreal injection of anti-VEGF agent is the first choice modality for macular edema due to CRVO in current clinical practice.^9–15 Although recent studies have demonstrated the efficacy and safety of ranibizumab and aflibercept,^9–11 frequent injections are required to obtain optimal outcome of the therapy, which may result in high cost of treatments. In actual clinical practice, the most frequently used anti-VEGF drug is bevacizumab.¹⁹ Epstein et al¹² reported, in a prospective randomized controlled study, that IVB given every 6 weeks for 12 months significantly improved visual acuity and reduced macular edema. Moreover, some retrospective studies^15–18 have reported that periodical or PRN use of IVB improved visual acuity and macular edema due to CRVO. Our results also demonstrate that IVB with a PRN regimen provides a substantial visual benefit in patients with macular edema secondary to CRVO. The patients gained mean vision of 0.25 logMAR units, which is compatible with those of previous reports^9–11 using fixed (monthly) injection regimen. In addition, the mean number of IVB injections in the current study (1.8 over a period of 6 months) was less than half of the monthly injections because the mean recurrence-free period was 2.6 months. Fewer injections likely have several benefits for the patients (ie, lower risk of complications, infrequent visits to the hospital). A previous report²⁰ described that the mean number of IVB injections with a PRN regimen was 1.8±0.8 over 6 months in branch retinal vein occlusion cases. Our multivariable analysis revealed that the duration from the onset of disease to the initial treatment was a significant prognostic factor for visual outcome. This result was consistent with previous reports^9–12,15 describing that delayed treatment with anti-VEGF agents resulted in limited visual improvement. Moreover, the current study, for the first time, demonstrated that the change in BCVA at 1 month was a significant prognostic factor associated with change in BCVA at 6 months in the CRVO patients. Therefore, earlier treatment and early response to the treatment are considered very important for the visual outcome of CRVO patients.

The limitation of the current study was that it was of a retrospective nature with a small population and a shorter follow-up period. Hence, it is not powered for safety of the treatment. The lack of control group is also a limitation of the study. Larger sample size and longer observation periods with prospective nature may lead to more precise estimates about the clinical indicators for the outcome of IVB on macular edema secondary to CRVO. However, our results suggest that some clinical information might be useful to predict the visual outcome of CRVO patients.

Conclusion

IVB with a PRN regimen significantly reduced macular edema and improved visual acuity of CRVO patients. Patients receiving earlier treatment had greater chance to obtain good visual outcome. The change in BCVA at 1 month was positively correlated with the change in BCVA at 6 months of follow-up.

Disclosure

The authors report no conflicts of interest in this work.