Macular sensitivity after half-dose verteporfin photodynamic therapy in central serous chorioretinopathy

Abstract

Objective

To study the macular sensitivity after half-dose verteporfin photodynamic therapy in patients with resolved central serous chorioretinopathy using the automated static perimeter.

Methods

Prospective consecutive case study of 24 patients with resolved central serous chorioretinopathy was performed. The macular sensitivity was measured using a conventional automated static perimeter with the Swedish interactive threshold algorithm 10-2 and foveal threshold. Best corrected visual acuity, intraocular pressure, fundus examination, macular thickness, and volume were also examined. The mean macular sensitivities of the affected eyes and their normal fellow eyes were calculated and compared. P<0.05 was considered statistically significant.

Results

The mean macular sensitivities of the affected eyes were lower than the normal fellow eyes with a statistically significant difference in all areas of the study (P<0.05). Best corrected visual acuity improved significantly from pretreatment (0.26±0.3 logMAR) to posttreatment (0.075±0.15 logMAR, P<0.05). Macular thicknesses in affected eyes were 230.66±67.34 μm and in the normal eyes were 238.33±92.26 μm (P=0.68). Macular volumes in affected eyes were 8.77±0.49 and in the normal eyes were 8.70±0.50 (P=0.60). These findings were not statistically significant.

Conclusion

Eyes with resolved central serous chorioretinopathy after half-dose verteporfin photodynamic therapy had lower macular sensitivity than normal fellow eyes. These findings agreed well with the previous microperimetric studies. The conventional automated static perimeter can also be used when a microperimeter is not available.

Keywords:

• macular sensitivity
• half-dose photodynamic therapy
• automated static perimeter
• central serous chorioretinopathy

Introduction

The neurosensory detachment of the macula in central serous chorioritinopathy (CSCR) is commonly reported in the middle-aged males.^1,2 The visual acuity (VA) is decreased, and the color perception is distorted, because the photoreceptor cells and retinal pigment epithelium (RPE) are destroyed from subretinal fluid that causes macular degeneration and RPE decompensation.³ Most of patients have conservative treatment and make spontaneous recovery.⁴ Only those who have prolonged duration of detachment of more than 12 weeks were treated with any interventions such as focal laser photocoagulation, anti-vascular endothelial growth factor (VEGF), or photodynamic therapy (PDT).^5–10

PDT with verteporfin has been recently used for treating CSCR, and studies have demonstrated beneficial visual outcomes in most patients. The mechanism of action is postulated to be choriocapillaris hypoperfusion and choroidal vascular remodeling, leading to reduction in choroidal congestion and vascular leakage. In some CSCR patients who have juxtafoveal or subfoveal leakage, it is hard to apply laser photocoagulation. Hence, PDT with verteporfin may have benefit for these patients in which it reduces the choroidal vascular hyperpermeability and promotes decreased leakage in the leaking area.⁷ The conventional PDT in CSCR patients, however, can result in potential complications such as RPE atrophy, choroidal ischemia, and secondary choroidal neovascularization (CNV).^8,9 Half-dose verteporfin photodynamic therapy (HD-PDT) has been reported to minimize this potential chorioretinal damage.¹⁰

Previous microperimetric studies showed that eyes with resolved CSCR had lower retinal sensitivity in the central macula than control eyes,^11–13 and there is a significant improvement in the macular sensitivity after HD-PDT when compared with the sensitivity before PDT treatment.^14–16 Because there was a significant correlation between microperimetry and automated static perimetry,¹⁷ it was hypothesized that the automated static perimeter, that is more commonly available in general practice, can be used to study macular sensitivity when a microperimeter is not available. Therefore, it was proposed to study the macular sensitivity after HD-PDT in patients with resolved CSCR using the automated static perimeter.

Methods

This study was performed in accordance with the tenets of the Declaration of Helsinki and was approved by Khon Kaen University Ethics Committee (Khon Kaen, Thailand) for human research (HE551117). Patients who had been diagnosed chronic CSCR and treated with HD-PDT at the KKU eye center from January 2012 until January 2014 were recruited for this study. Patients were older than 18 years old and had normal VA in the normal eyes, and were followed for at least 6 months. Those who had ocular diseases or previous ocular surgery were excluded. All study participants signed the informed consent forms.

All patients had unilateral CSCR and were treated with HD-PDT. Eyes with resolved CSCR were the study eyes and their normal fellow eyes were the control eyes. Patients had complete ocular examinations, which included the best corrected visual acuity (BCVA) with a Snellen chart that was converted to logarithm of the minimum angle of resolution (logMAR), intraocular pressures with Goldmann applanation tonometer, fundus examinations, macular thickness measurements, and volumes with an optical coherence tomographer (Spectralis, Heidelberg Engineering, Heidelberg, Germany). The macular sensitivity was measured using conventional automated static perimeter with the Swedish interactive threshold algorithm 10-2 and foveal threshold (Humphrey 750i field analyzer, Carl Zeiss Meditec, Dublin, CA, USA). The 68 stimulus points covering the central 10° in diameter were used. The mean macular sensitivities were then calculated in four different areas: center (foveal threshold), four points (1°) in a blue line, 16 points (3°) in a red line, and 36 points (5°) in a green line (Figure 1). The macular sensitivities were also calculated in four different quadrants: superotemporal, superonasal, inferotemporal, and inferotemporal from the fixation point covering the central 10° (Figure 2).

Figure 1 The areas to measure the macular sensitivity: center (foveal threshold), four points (blue line), 16 points (red line), and 36 points (green line) from fixation point.

Figure 2 The areas to measure the macular sensitivity: superotemporal, superonasal, inferotemporal, and inferotemporal from fixation point.

PDT was performed using a 3 mg/m² body surface area for the verteporfin dosage (Visudyne, Novartis International AG, Bülach, Switzerland) which is half the conventional dose of verteporfin. Verteporfin was infused over a 10-minute period followed by delivery of 50 J/cm² from a 689 nm laser system (Carl Zeiss Meditec) over an 83-second exposure period. The size of the laser spot was the diameter of the region of indocyanine green hyperpermeability.

The Stata for Windows, version 10 was used for the statistical analysis. The mean retinal sensitivity, macular thickness, and macular volume of the study eyes were compared with the control eyes using paired Student’s t-test. The BCVA before and after HD-PDT was also compared using paired t-test. P<0.05 was considered statistically significant.

Results

Of the 24 affected eyes in 24 patients recruited for the study, 15 eyes were on the left side. Seventeen patients were male, and the median age was 46 years old (36–68 years). The median duration of pre-PDT was 32 weeks (15–96 weeks) and post-PDT was 126 weeks (48–216 weeks). The demographic and clinical data of the patients are shown in Table 1.

Table 1 Demographic and clinical characteristics of the patients (n=24)

Characteristics	Data
Demographic data
Age (median, min–max) years	46, 36–68
Sex: male/female	17/7
Laterality: right eye/left eye	15/9
Education above high school (number of patients)	7
Clinical characteristics
Duration before PDT (median, min–max) weeks	32, 15–96
Duration after PDT (median, min–max) weeks	126, 48–216
site of leakage: subfoveal leakage	2
Juxtafoveal leakage	19
Extrafoveal leakage	3
Spot size (median, min–max) µm	2,216, 1,089–4,398

Abbreviations: PDT, photodynamic therapy; min, minimum; max, maximum.

BCVA improved significantly from pretreatment (0.26±0.3 logMAR) to posttreatment (0.075±0.15 logMAR, P<0.05). The mean macular sensitivities in each area of the study are shown in Table 2. It was noted that the eyes with resolved CSCR after HD-PDT have lower macular sensitivity than their normal fellow eyes with a statistically significant difference.

Table 2 The comparative mean macular sensitivities in study and control eyes: four lines; foveal threshold, four, 16, and 36 points from fixation and four areas; superotemporal, superonasal, inferotemporal, and inferonasal areas

Study areas	Mean macular sensitivity		P-value
	Study eyes (decibel)	Control eyes (decibel)
Center: foveal threshold	28.75±6.52	32.33±3.35	<0.001
Blue line, four points	29.27±3.33	30.92±1.49	<0.001
Red line, 16 points	29.28±3.34	31.02±1.67	<0.001
Green line, 36 points	28.85±3.22	30.67±1.65	<0.001
Superotemporal area	28.56±3.01	30.43±1.15	<0.001
Superonasal area	28.23±3.18	30.22±1.21	<0.001
Inferotemporal area	29.11±2.86	30.81 ± 1.49	<0.001
Inferonasal area	29.35±2.02	30.44± 1.49	0.002

Note: Data presented as mean ± SD.

Macular thicknesses of the study eyes were 230.66± 67.34 µm, and of the normal eyes were 238.33±92.26 µm (P=0.68). Macular volumes in study eyes were 8.77±0.49, and normal eyes were 8.70±0.50 (P=0.60). These findings were not statistically significant. Five patients had loss the inner segment/outer segment (IS/OS) junction, two patients had persistent subretinal fluid, and two patients presented with pigment epithelial detachment (PED).

Discussion

Many modalities of treatment are used for visual improvement in patients with CSCR although most of the patients resolved spontaneously. Several studies have reported that patients treated with PDT have favorable outcomes both anatomically and functionally.^7–10 Maruko et al evaluated the subfoveal choroidal thickness after PDT treatment of CSCR, visualized by enhanced depth imaging spectral domain optical coherence tomography. They demonstrated that the choroidal thickness was reduced after PDT and suggested that PDT may reduce the choroidal vascular hyperpermeability in CSC.⁷ Potential complications after PDT such as RPE atrophy, choroidal ischemia, and secondary CNV had been reported.^8,9 All these complications affected the macular sensitivity. Chan et al¹⁰ reported HD-PDT for CSCR patients to minimize these chorioretinal damages. They demonstrated that decreasing the dose of verteporfin to be half-dose maintained its efficacy and improved safety.

This study demonstrates that using the automated static perimeter, eyes with resolved CSCR after HD-PDT, have lower retinal sensitivity in the central macula than their normal fellow eyes with a statistically significant difference. This finding agrees well with the previous microperimetric studies. Ozdemir et al¹¹ studied macular function by microperimetry in eyes with CSCR and showed significantly lower retinal sensitivity not only at the central but also in the paracentral area. They also showed that eyes with resolved CSCR had lower retinal sensitivity in the central macula than control eyes, even after good VA has been obtained.¹² Ojima et al¹³ measured retinal sensitivity with microperimeter 1 after resolution of CSCR and reported that eyes with resolved CSCR had focal areas with reduced retinal function, which is consistent with irregularity of the RPE or with defects of the IS/OS.

Sekine et al¹⁸ studied the retinal sensitivity in eyes with untreated CSCR using automated static perimeters. They demonstrated lower retinal sensitivity in the central macula and proposed that macular sensitivity was attenuated by increasing the distance between the photoreceptor and RPE. Springer et al¹⁷ performed static threshold perimetry in 30 healthy volunteers comparing between the microperimeter and automated static perimeter and demonstrated that sensitivity values in microperimetry are comparable to the threshold values obtained with the automated static perimeter (Octopus 101). Therefore, automated static perimetry can be used to measure the macular sensitivity when a microperimeter was not available.

This study revealed comparative point-to-point macular sensitivity in both CSCR and normal fellow eyes with the automated static perimeter, and demonstrated the decrease in macular sensitivity in all areas that HD-PDT was applied despite good anatomic reattachment and normal VA. This may be explained by the result of the photoreceptors and RPE damage from chronic macular detachment. PDT itself had also been reported to cause RPE damage.⁹ Therefore, the reduced macular sensitivity may be affected from both chronic macular detachment and PDT.

Although many published papers have shown that PDT gave good visual results in acute and chronic CSCR,^7–10 there were also some risks of developing CNV, choroidal ischemia, RPE atrophy, PED, RPE rip, and loss of the IS/ OS junction.^8,9,19,20 These late complications need to be monitored in long-term follow-up. Either microperimeter or automated static perimeter can be used. Because the latter is more commonly available in general practice, it is an alternative to use in monitoring these CSCR patients.

Conclusion

Eyes with resolved CSCR after HD-PDT had lower retinal sensitivity in the central macula than in normal fellow eyes. This finding agrees well with the previous microperimetric studies. A conventional automated static perimeter can also be used when a microperimeter is not available.

Acknowledgments

The authors thank Dr Kaewjai Thepsuthammarat, Clinical Epidemiology Unit, Faculty of Medicine, Khon Kaen University for statistical analysis and Professor James A Will, University of Wisconsin for assistance with the English-language presentation of the manuscript.

Disclosure

The authors report no conflicts of interest in this work.