Abstract
Recent studies demonstrate that microRNA-related single-nucleotide polymorphisms (miR-SNPs) are associated with the development of numerous human cancers. In this study, we investigated six miR-SNPs in microRNA processing machinery genes, including rs11077 of the XPO5 gene, rs14035 of the RAN gene, rs3742330 of the Dicer gene, rs9623117 of the TNRC6B gene, rs197412 of the GEMIN3 gene, and rs2740348 of the GEMIN4 gene, in gastric cancer patients and subsequently evaluated their potential roles in gastric cancer risk in a case control study. The results indicate that the C/C genotype of rs14035 from RAN, the A/A genotype of rs3742330 from Dicer, and the T/T genotype of rs9623117 from TNRC6B are significantly associated with gastric cancer risk. In conclusion, these miR-SNPs can be used as predictive biomarkers in gastric cancer.
Keywords:
Introduction
Gastric cancer (GC) is the fourth-most-common cancer and the second leading cause of cancer-related deaths worldwide;Citation1,Citation2 in addition, nearly two-thirds of GCs occur in developing regions.Citation3 As a multifactorial disease, both environmental factors and genetic factors contribute to the etiology of this disease.Citation4,Citation5 Although much treatment progress has been achieved in recent years, the prognosis of GC remains poor due to the delay in diagnosis.Citation6 MicroRNAs (miRNAs) are RNA molecules with lengths of ~22 nucleotides; they can act as posttranscriptional regulators in mRNA expression, and they regulate a variety of physiological and developmental processes, including the development and progression of numerous cancers.Citation7–Citation13 In GC, miRNAs can serve as biomarkers for both diagnosis and therapeutic targets.Citation14–Citation17
In miRNA processing, long primary transcripts of miRNAs (pre-miRNAs) are synthesized by RNase II in the nucleus, and they are cut into an ~70 nt hairpin precursor by RNase III Drosha and by the double-stranded RNA-binding protein DiGeorge syndrome critical region 8 (DGCR8). These precursors are exported to the cytoplasm, are processed by exportin-5 (XPO5) and RAN-GTPase (RAN), and are further diced into ~22 nt miRNA duplexes by the RNase III Dicer gene, which cooperates with the transactivation response RNA-binding protein and the Argonaute protein family. MiRNA duplexes are then assembled into the miRNA-induced silencing complex (miRISC), which contains GEMIN3 and GEMIN4. The miRISC will select one strand as the mature miRNA, and it guides the mature miRNAs to their target mRNA sites.Citation18–Citation23 MiRNA-related single nucleotide polymorphisms (miR-SNPs), which are defined as single-nucleotide polymorphisms (SNPs) in miRNA genes at the miRNA binding site and in miRNA processing machinery, can modulate miRNA and target gene expression to influence cancer development, promote therapeutic efficacy, and affect a patient’s prognosis.Citation23–Citation26 The miRNA machinery genes are linked to the development, progression, and prognosis of several cancer types, including ovarian and colorectal cancers, melanoma, and T-cell lymphoma.Citation27–Citation30 However, the role of the miRNA processing machinery genes in GC remains uncertain.
In the present study, we genotyped six miR-SNPs in the miRNA processing machinery genes, including XPO5 (rs11077), RAN (rs14035), Dicer (rs3742330), TNRC6B (rs9623117), GEMIN3 (rs197412), and GEMIN4 (rs2740348) in GC patients to evaluate the relationships of these genes with risk for development of GC.
Materials and methods
Sample collection and DNA extraction
Blood samples were collected from GC patients who underwent GC resection at the Department of General Surgery at the Fourth Hospital of Hebei Medical University from 2007–2008. Blood samples were also collected from normal controls without a history of any cancer. Genomic DNA was immediately extracted using the Wizard® Genomic DNA Purification Kit (Promega Corporation, Fitchburg, WI, USA) and was stored at −20°C. All procedures were supervised and approved by the Human Tissue Research Committee of the Fourth Hospital of Hebei Medical University. Written consent was obtained from all the patients and healthy controls enrolled in this study.
Genotyping of miR-SNPs
The miR-SNPs of the miRNA processing gene, including XPO5 (rs11077), RAN (rs14035), Dicer (rs3742330), TNRC6B (rs9623117), GEMIN3 (rs197412), and GEMIN4 (rs2740348), according to the National Center for Biotechnology Information (NCBI) SNP database (http://www.ncbi.nlm.nih.gov/snp/), were genotyped using the polymerase chain reaction (PCR)–ligase detection reaction assay with the primers and probes listed in . PCR was performed with the PCR Master Mix Kit according to the manufacturer’s instructions (Promega, Madison, WI, USA). Ligation was performed using the different probes that were matched to the miR-SNPs, and the ligated products were separated using the ABI PRISM Genetic Analyzer 3730XL (Applied Biosystems, Foster City, CA, USA) to access the length differences of the ligated products.
Table 1 All the primers and probes used for genotyping of miR-SNPs
Statistical analysis
The χ2 test was applied to analyze dichotomous variables, such as the presence or absence of an individual SNP in patients and healthy controls. The odds ratios and 95% confidence intervals (CI) were calculated using an unconditional logistic regression model. All of the statistical analyses were performed with the SPSS version 18.0 software package (IBM Corporation, Armonk, NY, USA). A P-value <0.05 was considered statistically significant.
Results
A total of 137 GC patients and 142 healthy controls were included in this study. The clinical characteristics of the cases and controls are listed in . Six miR-SNPs were detected from the blood samples of the healthy controls and GC patients.
Table 2 Clinical characteristics in patients with gastric cancer and controls
When individual SNPs were compared between GC patients and controls in terms of their distribution frequency, statistically significant increases for the C/C genotype of rs14035 in the RAN gene (95% CI: 1.601–14.925; P=0.002), the T/T genotype of rs9623117 in the TNRC6B gene (95% CI: 0.03–0.608; P=0.003), and the A/A genotype of rs3742330 in the Dicer gene (95% CI: 1.223–3.178; P=0.005) were observed in GC patients. These results indicate that the carriers of these alleles are susceptible to GC ().
Table 3 miR-SNPs frequency difference between patients with gastric cancer and controls
Discussion
The miR-SNPs in RAN, Dicer, and TNRC6B were associated with the carcinogenesis of GC in our analysis. To our knowledge, this is the first report to indicate that the SNP sites in the miRNA processing machinery genes have predictive value for determining the incidence of GC.
RAN is a member of the Ras superfamily of GTPases and is essential for the translocation of pre-miRNAs from the nucleus to the cytoplasm. XPO5 binds to pre-miRNA and RAN-GTPase in the nucleus (via the XPO5–RAN GTP–pre-miRNA heteroternary complex) to mediate the nuclear export of pre-miRNA in a RAN GTP-dependent manner.Citation31 Disruption of pre-miRNA nucleocytoplasmic transport would impair the production of mature miRNAs in cancer cells. The fact that RAN is overexpressed in some cancer cell lines, including colon cancer, implies its role in tumor transformation; moreover, RAN was reported to suppress the activation of C-Jun-NH2-kinase and inhibit the apoptosis induced by an anticancer drug.Citation32,Citation33 The rs14035 located in RAN might alter RAN expression, so as to initiate carcinogenesis by modulating the production of mature miRNAs.
Dicer was also implicated in the oncogenic process of several cancers, but the data were controversial; downregulated Dicer expression has been identified in lung cancer, ovarian cancer, nasopharyngeal cancers, breast cancer, and esophageal cancer, whereas upregulated Dicer expression was found in lung adenocarcinoma, colorectal cancer, and primary cutaneous T-cell lymphomas.Citation27,Citation34–Citation40 The mechanism underlying how the rs3742330 SNP modified the GC risk remains unclear; the location of this SNP in the 3′-untranslated region of Dicer might potentially influence the stability and expression of the gene.
TNRC6B (or KIAA1093), localized on the mRNA-degrading cytoplasmic P bodies, is one of the three Argonaute-interacting protein paralogs in vertebrates. They assist in the formation of miRNA ribonucleoparticles or miRISCs, so as to mediate miRNA-guided mRNA cleavage.Citation16,Citation41–Citation44 The T/T genotype of rs9623117 in TNRC6B has been found to be associated with prostate cancer risk. Alterations in TNRC6B gene expression due to genetic variations of rs9623117 might perturb the levels of miRNA species normally under its control, thus contributing to carcinogenesis.Citation45
The frequency distribution of these six SNPs and the patients’ clinical characteristics (including their sex, age, and tumor stage) do not appear to be associated, as determined by our analysis (data not shown). These six miR-SNPs were analyzed for their relationship with postoperative survival in 95 patients for whom 3-year follow-up data were available. It was found that rs2740348 showed a marginally statistically significant association with survival (P=0.06; our unpublished data). These findings should be validated with a larger sample size.
Although the results of this study require further validation among a larger GC cohort, as well as in laboratory-based functional studies, our data are encouraging because they demonstrate that miR-SNPs can be used to predict the risk for developing GC.
Author contributions
All authors made substantial contributions to data generation and analysis, drafting or critical revision of the manuscript, and approval for the final version to be published.
Acknowledgments
This work was supported by the Key basic research program of Hebei (14967713D).
Disclosure
The authors report no conflicts of interest in this work.
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