Dear editor
We have read the published article, and we appreciate the authors who have reported the article about “Alginate Beads as a Promising Tool for Successful Production of Viable and Pluripotent Human-Induced Pluripotent Stem Cells in a 3D Culture System”, published in Stem Cells and Cloning: Advances and Applications s 2023:16 61–73. We appreciate the success of Alsubaie et al in carrying out 3D culture using the encapsulation technique with alginate and gelatin on induced pluripotent cell/iPSCs so that they can suppress their teratoma properties.Citation1
The encapsulation process is carried out on stem cells such as induced pluripotent cell/iPSC and mesenchymal stem cell/MSC to make them more stable, resistant to temperature and maintain their viability.Citation1,Citation2 Previous research has shown the antibacterial hydrogel was able to kill approximately 99% of the exposed bacteria after 3 h of exposure. The developed antibacterial hydrogels are light weight, have a high water-uptake capacity, and show high biocompatibility with the model mammalian cells, which make them a promising candidate to be used for wound dressing applications.Citation3
This study directly Human IPSCs under dynamic 3D culture were able to secrete the necessary ECM components to form a suitable niche, which might help to maintain the pluripotent state. Meanwhile, in our opinion, it is also necessary to mention Dynamic 3D culture is desirable for the large-scale expansion of undifferentiated human IPSCs for what therapy or research expansion applications. We suggest using encapsulated herbs such as lemongrass and various herbs to protect cell DNA when large-scale expansion is carried out.Citation4
Disclosure
The authors report no conflicts of interest in this communication.
References
- Alsobaie S, Alsobaie T, Alshammary AF, Abudawood A, Mantalaris A. Alginate beads as a promising tool for successful production of viable and pluripotent human-induced pluripotent stem cells in a 3D culture system. Stem Cells Cloning. 2023;16:61–73. doi:10.2147/SCCAA.S409139
- Harlim A, Rilianawati E. Optimization of alginate-based encapsulation utilization for viability and stability of the mesenchymal stem cell. J Stem Cell Res Ther. 2018;8:412. doi:10.4172/2157-7633.1000412
- Tavakolian M, Munguia-Lopez JG, Valiei A, et al. Highly absorbent antibacterial and biofilm-disrupting hydrogels from cellulose for wound dressing applications. ACS Appl Mater Interfaces. 2020;12:39991–40001. doi:10.1021/acsami.0c08784
- Khorshidian N, Mahboubi A, Kalantari N, et al. Chitosan-coated alginate microcapsules loaded with herbal galactagogue extract: formulation optimization and characterization. Iran J Pharm Res. 2019;18(3):1180–1195. doi:10.22037/ijpr.2019.1100776