Sex differences in clinical characteristics and 1-year outcomes of young ischemic stroke patients in East China

Abstract

Background

Data concerning sex differences in clinical characteristics and outcomes of young ischemic stroke (IS) patients in Eastern China are scarce. Understanding sex differences in clinical characteristics and long-term outcomes of young IS patients might provide valuable evidence for designing preventative measures and therapeutic interventions.

Methods

The study included 228 acute IS patients aged up to 50 years recruited in the prospective Nanjing First Hospital Stroke Registry over a 5-year period. Univariable and multivariable logistic regression analyses were performed to determine whether there were sex differences in clinical characteristics and outcomes of young IS patients.

Results

Admission systolic blood pressure (130.12±24.3 vs 137.96±24.3 mmHg, P=0.005) of women was significantly lower than that of men. Logistic regression showed that young women had poorer outcomes defined as having modified Rankin Scale score of 3–6 at 12 months after the adjustment for history of prior stroke, NIHSS score, and complication of pneumonia (adjusted OR: 3.45; 95% CI: 1.43–8.32).

Conclusion

Our study indicates that there may be significant differences in clinical characteristics between young women and men with acute IS in East China. Young women were more likely to be dead or dependent at 12 months after stroke onset. More attention should be paid to young women’s IS prevention and management in East China.

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Keywords:

• sex differences
• young ischemic stroke
• clinical characteristics
• outcomes

Introduction

In China, stroke has become the leading cause of death and disability.¹ Ischemic stroke (IS) has been reported to be the most common type of stroke, and its incidence in young adults has been significantly increasing.² The long-term mortality after stroke in young adults is higher compared with expected mortality in the general population according to a recent research.³

It is of vital importance to study the characteristics and outcomes of these young Chinese stroke patients with a view toward developing tailored prevention programs. There were few studies that showed sex differences in clinical characteristics and outcomes of IS patients.^4,5 Rutten-Jacobs et al assessed the survival status of 606 young IS patients and found that 20-year mortality was higher in male than in female stroke survivors.³ On the other hand, another study revealed that young women have poorer outcomes than men after IS.⁶ Sex differences in young IS in China have not been previously evaluated.

We explored sex differences in clinical characteristics and 1-year outcomes of young IS patients using data from a prospective and long-term study of stroke registry in East China.

Methods

Subjects

Consecutive patients with acute stroke were prospectively recruited from August 2004 to August 2008 in the Nanjing First Hospital Stroke Registry (NFHSR), which was a hospital-based and prospective registry study of stroke patients with 1-year follow-up. Stroke was defined as acute IS, intracerebral hemorrhage, or subarachnoid hemorrhage, and the diagnosis was made according to the WHO criteria and confirmed by brain computed tomography or magnetic resonance imaging scans.⁷ Patients diagnosed with stroke within 14 days of onset were eligible to be enrolled and were followed up for 1 year. In the current study, IS patients aged 18–50 years were included if baseline information and data of 12-month follow-up were available.³ This study was approved by the Ethics Committee of Nanjing First Hospital and conducted in accordance with the Declaration of Helsinki, and we obtained written informed consent from all participants or their guardians.

Data collection

All IS patients were managed according to standard stroke care guidelines in the neurology department of Nanjing First Hospital. In the present study, we collected baseline patient information including age, sex, onset-to-door time (ODT), pre-stroke dependency, stroke severity, laboratory tests (admission hemoglobin, blood glucose, triglyceride, and cholesterol), brain imaging data, as well as other risk factors for IS. Pre-stroke dependency was defined as having a modified Rankin Scale (mRS) score of 3–5.⁸ The severity of IS was measured by National Institutes of Health Stroke Scale (NIHSS) and Glasgow Coma Scale (GCS).^9,10 Having a GCS score of <9 indicated serious disturbance of consciousness. Other risk factors for IS included the history of hypertension (defined as self-reported history of hypertension, or taking oral antihypertension drugs), diabetes mellitus (defined as self-reported history of diabetes mellitus, or taking hypoglycemic agents), hyperlipidemia (defined as self-reported history of hyperlipidemia, or taking oral antihyperlipidemia drugs), atrial fibrillation (defined as history of atrial fibrillation, confirmed by at least one electrocardiogram, or the presence of the arrhythmia after admittance), coronary heart disease, and prior stroke (defined as a confirmed medical history of IS or intracerebral hemorrhage), current smokers (defined as patients who had smoked >1 cigarette/day for over 1 year), and current drinkers (defined as patients who had consumed alcohol >50 mL/day for at least 1 year). In addition, patients’ systolic and diastolic blood pressure (SBP and DBP) at admission and the in-hospitalization complications were also recorded.

Outcomes

Primary outcomes at 12 months after enrollment were death or dependency as evaluated by the mRS and stroke recurrence. Death or dependency was prospectively evaluated through telephone follow-ups. Death was defined as all-cause cumulative mortality. Dependency was defined as having a mRS score of 3–5. Stroke patients were also followed prospectively to assess the recurrent stroke rate.

Statistical analyses

A comparison of categorical variables was conducted using chi-squared test. Normally distributed continuous variables including age, blood pressure, and hemoglobin were assessed by Student’s t-test, and variables with skewed distribution, for example, ODT, GCS score, and NIHSS score at admission were compared using Wilcoxon test. The association between sex and clinical outcomes at 12 months was ascertained by univariable and multivariable logistic regression models. All variables with P≤0.2 in the univariate analyses were included in the multivariate model. SPSS version 16.0 (SPSS Inc., Chicago, IL, USA) was used for all statistical analyses. A two-sided P-value of <0.05 was considered statistically significant.

Results

Baseline characteristics, complications, and outcome of young patients

Figure 1 shows the patient selection flowchart. Over the 5-year study period, 3,412 consecutive acute cerebrovascular disease patients within 14 days of onset were included in NFHSR. After exclusion of patients who were diagnosed with intracerebral hemorrhage, subarachnoid hemorrhage, and transient ischemic attack, 2,318 cases of IS were followed up for 12 months. There were altogether 245 young IS patients aged from 18 to 50 years. Seventeen (6.9%) cases were lost to follow-up, and the remaining 228 (93.1%) patients (81 women [35.5%] and 147 men [64.5%]) were included for the present analyses.

Figure 1 Flowchart of patient selection.

The mean age of all patients was 42.61±6.2 years, and it was not significantly different between women and men (42.31±6.6 vs 42.78±6.0 years, P=0.563). Table 1 demonstrates the sex-specific characteristics and outcome of young patients with IS. Compared with men, women had lower SBP (130.12±24.3 vs 137.96±24.3 mmHg, P=0.005), DBP (81.30±15.4 vs 85.92±16.7 mmHg, P=0.004), hemoglobin (129.60±14.9 vs 147.96±16.3 g/L, P<0.001), and triglyceride (1.61±0.8 vs 1.97±1.6 mmol/L, P=0.047) and lower GCS score on admission (15 [14, 15] vs 15 [15, 15], P=0.043). In addition, hypertension (19.8% vs 34.0%, P=0.013) was less frequent in women than in men, whereas atrial fibrillation (14.8% vs 5.4%, P=0.006) and severe stroke (defined as having NIHSS score >14; 17.3% vs 5.4%, P=0.003) were more frequent in women. Furthermore, women were significantly less likely to smoke (5.0% vs 51.7%, P<0.001) and drink (2.5% vs 37.4%, P<0.001) than men. Regarding outcome, women had significantly higher proportion of death or dependency (28.4% vs 11.6%, P=0.001) and recurrent stroke (7.4% vs 1.4%, P=0.025) at 12 months than men.

Table 1 Sex differences of baseline characteristics, complications, and outcome

Variables	Female (N=81)	Male (N=147)	P-value
Baseline characteristics
Age (years)	42.31±6.6	42.78±6.0	0.563
Hypertension	16 (19.8)	50 (34.0)	0.013
SBP at admission (mmHg)	130.12±24.3	137.96±24.3	0.005
DBP at admission (mmHg)	81.30±15.4	85.92±16.7	0.004
Hemoglobin (g/L)	129.60±14.9	147.96±16.3	<0.001
Diabetes mellitus	4 (5.0)	13 (8.8)	0.259
Random blood glucose	6.24±2.7	6.50±4.4	0.596
Hyperlipidemia	1 (1.0)	8 (5.4)	0.052
Triglyceride (mmol/L)	1.61±0.8	1.97±1.6	0.047
Cholesterol (mmol/L)	4.41±1.0	4.66±1.2	0.114
Atrial fibrillation	12 (14.8)	8 (5.4)	0.006
Coronary heart disease	1 (1.0)	1 (0.7)	0.696
Prior stroke	5 (6.2)	8 (5.4)	0.947
Pre-stroke dependencya	5 (6.2)	7 (4.8)	0.625
Current smokers	4 (5.0)	76 (51.7)	<0.001
Current drinkers	2 (2.5)	55 (37.4)	<0.001
Median OTD time (hours)	48 [20, 120]	48 [10.5, 120]	0.466
Median GCS scoreb	15 [14, 15]	15 [15, 15]	0.043
GCS score <9	6 (7.4)	5 (3.4)	0.104
Median NIHSS scorec	4 [2, 10]	4 [1, 8]	0.052
NIHSS score >14	14 (17.3)	8 (5.4)	0.003
In-hospitalization complications
Gastrointestinal hemorrhage	0 (0)	4 (2.7)	0.086
Urinary tract infection	2 (2.5)	2 (1.4)	0.579
Pneumonia	8 (9.9)	17 (11.6)	0.683
Outcome at 12 month
Death or dependency	23 (28.4)	17 (11.6)	0.001

Notes: Data are mean ± SD, median [IQR] or n (%).

a Pre-stroke dependency is defined as the modified Rankin Scale scores of 3–5;

b GCS scores can range from 3 (deep coma) to 15 (healthy);

c NIHSS scores can range from 0 (healthy) to 42 (coma with quadriplegia).

Abbreviations: DBP, diastolic blood pressure; GCS, Glasgow Coma Scale; IQR, interquartile range; NIHSS, National Institutes of Health Stroke Scale; OTD, onset to door; SBP, systolic blood pressure.

Sex-specific outcomes at 12 months after IS

Results of univariable and multivariable analyses of associations between sex and 12-month outcome are shown in Table 2. Without adjustment for potential confounders, there were significant sex differences in death or dependency after IS (OR: 3.03; 95% CI: 1.51–6.10). After adjustment for the potential confounders including prior stroke, NIHSS score, and complication of pneumonia, we found that female sex was still an independent predictor for poor outcomes at 12 months (adjusted OR: 3.45; 95% CI: 1.43–8.32). Other independent predictors for poor outcomes at 12 months were: NIHSS score (adjusted OR: 1.18; 95% CI: 1.10–1.28) and pneumonia (adjusted OR: 3.75; 95% CI: 1.01–13.90).

Table 2 Factors associated with death or dependency in young ischemic stroke patients at 12 months after onset

Variables	Unadjusted OR (95% CI)	Adjusted OR (95% CI)
Demographic data and vascular risk factors
Age (years)	1.02 (0.96–1.08)	–
Female sexa	3.03 (1.51–6.10)	3.45 (1.43–8.32)*
Pre-stroke dependency	2.19 (0.27–17.62)	–
Prior strokea	0.44 (0.14–1.33)	1.65 (0.39–7.01)
Hypertension	1.34 (0.61–2.92)	–
Diabetes mellitus	0.61 (0.19–2.01)	–
Atrial fibrillation	0.52 (0.17–1.55)	–
Coronary heart disease	0.21 (0.01–3.41)	–
Hyperlipidemia	1.51 (0.18–12.61)	–
Current smoker	1.29 (0.63–2.66)	–
Current drinker	1.25 (0.56–2.80)	–
Clinical features at admission
NIHSS scorea	1.22 (1.14–1.31)	1.18 (1.10–1.28)*
GCS scoreb	0.61 (0.50–0.73)	–
OTD time ≥3 hours	1.78 (0.60–5.25)	–
In-hospitalization complications
Pneumoniaa	8.52 (3.29–22.08)	3.75 (1.01–13.90)*
Urinary tract infection	2.39 (0.21–26.96)	–

Notes:

a Variables were selected for the multivariate analysis as P-value of the variable was not more than 0.2 in univariate analysis;

b Though the P-value of GCS score in univariate analysis was <0.2, GCS score was not involved in multivariate analysis for avoidance of collinearity with NIHSS score;

* Significant P<0.05.

Abbreviations: GCS, Glasgow Coma Scale; NIHSS, National Institutes of Health Stroke Scale; OTD, onset-to-door.

Discussion

Few studies in the past had explored sex differences in the outcomes of younger IS patients, and controversy still exists. Notably, there has been no knowledge about sex differences of long-term outcomes on the eastern Chinese population. We used data from the prospective, long-term and hospital-based registry to elucidate sex differences of young adults with IS in baseline characteristics and long-term outcomes in East China.

Sex differences in characteristics of young IS patients

An extensive knowledge of stroke risk factors in the specific population is essential for the development of efficient primary and secondary preventive strategies. The consecutive patients enrolled in NFHSR were representative of the local IS patients since over 80% stroke patients in the Qinhuai District, Nanjing City were admitted to Nanjing First Hospital. However, our results with respect to clinical characteristics of the stroke patients were not entirely similar to the previous reports.^6,11–14,21

Recently, published studies were in agreement that dyslipidemia was more prevalent in young men compared with women.^6,11–14 However, no significant sex difference in proportion of patients with hyperlipidemia was observed in this study. Our research demonstrated that hypertension was prevalent among young men, as reported in some studies.^6,13,14,21 Our finding of higher rates of atrial fibrillation among female stroke survivors is in agreement with the results of a prior study by Wang et al.⁴ The reason for the higher rates of atrial fibrillation among young females in China is not clear. Less physical activity, higher BMI, more stress, and hormone difference in Chinese young female may be contributing factors, warranting further exploration. In this study, there was a significantly greater proportion of current smokers and drinkers in male IS patients, which was previously shown not only in China but also in Spain and Finland.^13,6,14 A cross-sectional study in North-West Iran demonstrated that there was a significant difference in diabetes rates between men and women.²¹ However, there was no significant sex difference in the diabetes rates in our study of young IS only. These data showing gender variations in vascular risk factors emphasized the need to carry out evidence-based stroke prevention measures targeting sex-specific risk factors in young adults.

The discrepancies in study findings could be due to several reasons. First, the definition of young patient, especially age cutoff, varied in previous studies. Specifically, published studies and registries commonly defined young adults as those younger than 45 or 50 years. In addition, different research background, inclusion criteria of patients, and adoptive statistical methods could also result in discrepancy in study findings. Therefore, sex differences in emerging modifiable risk factors from various populations suggested that the prevention strategies should also differ by sex along with race and ethnicity in the younger IS group.

Sex differences in 12-month outcomes after IS

Past studies on young stroke patients have mostly focused on mortality as an outcome but not functional outcomes.^13,15–17 To our knowledge, few existing studies analyzed the sex differences in functional outcomes in young IS patients after controlling for potential confounding factors.^6,13 In our study, both death and dependency as outcomes at 12 months after onset were recorded. In accordance with the report by Martínez-Sánchez et al, the multivariable logistic regression analysis herein suggested that young women were more likely to be dead or dependent than men at the time of follow-up.⁶

In addition, our study showed that the higher NIHSS scores at admission, the worse functional outcomes at 12-month follow-up, similar to those in previous series.^6,13,18,19 Young women had a higher prevalence of atrial fibrillation in our study, which caused more recurrent stroke and cardiogenic stroke in our study. Compared with other causes of stroke, cardiogenic stroke had worse NIHSS at presentation and worse outcomes after stroke. Moreover, young patients complicated with pneumonia would have higher prevalence of death or dependency after the adjustment for sex, prior stroke, and NIHSS score. Heikinheimo et al²⁰ reported a study of 681 IS patients aged 15–49 years and found that poststroke infections comprising mostly pneumonia were associated with unfavorable short-term outcomes and higher long-term mortality, which is consistent with our research. Taken together, these results of data analysis reflect that aggressive management of severe female patients and prevention of pulmonary infection after stroke are of great importance.

Strengths and limitations

Our study was conducted using hospital-based cohort data with strengths including its prospective design, long-term follow-up, high follow-up rate, and good representation of local young patients with IS. The complete clinical characteristics and 12-month follow-up data enabled us to investigate sex differences of young adults with IS, which was not explored previously in East China.

However, there were some limitations in present study as described below. NFHSR is a single-center and teaching hospital-based stroke registry in Nanjing, the number of patients included is relatively small, and not all young IS patients in East China were included, which might bias the results. Also, potential confounding factors such as patients’ economic status, in-hospital special management (thrombectomy or intravenous thrombolysis), and posthospital management that may have influenced the sex disparity in long-term outcomes were lacking. However, special management strategies such as thrombectomy or intravenous thrombolysis may not have influenced our result as those treatments were not available in our hospital during 2004–2008. Due to the evolution of treatment options over the recent years, there might be a difference in results from more recent data (eg, prescribing of novel oral anticoagulants, intravenous thrombolysis, and mechanical thrombectomy increased, so the number of severe ISs might decrease over time).

Conclusion

Our study demonstrated that there may be significant sex differences of young IS patients in clinical characteristics and long-term outcomes in East China. Specifically, young women were more likely to be dead or dependent than men at 12-month follow-up, even after adjustment for confounding factors. More attention should be paid to the prevention and management of IS in young women in East China.

Acknowledgments

The work was supported by grants from National Natural Science Foundation of China (81471199), China Youth Clinical Research Fund (2017-CCA-VG-029), and Sichuan Science and Technology Program (2018JY0026). The funders had no role in the planning of the study or interpretation of the results. We would like to also express our sincere gratitude to the patients and their families who were involved in this registry study, and the clinical and research teams of the neurology department. The abstract of this paper was presented as a poster at the 2017 Asia-Pacific Stroke Conference as a poster; Oct 26–29, 2017; Nanjing. It was published in “Poster Abstracts” in Cerebrovascular Diseases 2017;44(Suppl 1):1–52 DOI: 10.1159/000481589.

Disclosure

The authors report no conflicts of interest in this work.