Optimizing endothelin receptor antagonist use in the management of pulmonary arterial hypertension

Abstract

Endothelin receptor antagonism has emerged as an important therapeutic approach in pulmonary arterial hypertension (PAH). Bench to bedside scientific research has shown that endothelin-1 (ET-1) is overexpressed in several forms of pulmonary vascular disease and may play an important pathogenetic role in the development and progression of PAH. Oral endothelin receptor antagonists (ERAs) improved exercise capacity, functional status, pulmonary hemodymanics, and delayed the time to clinical worsening in several randomized placebo-controlled trials. Two ERAs are currently approved by the US Food and Drug Administration: bosentan, a dual ERA for patients with class III and IV PAH, and ambrisentan, a selective ERA for patients with class II and III PAH. Sitaxsentan, another selective ERA, has been approved in Europe, Canada, and Australia. The objective of this review is to evaluate the available evidence describing the pharmacology, efficacy, safety, and tolerability, and patient-focused perspectives regarding the different types of endothelin receptor antagonists. Ongoing and forthcoming randomized trials are also highlighted including the approach of combining this class of drugs with other drugs that target different cellular pathways believed to be etiologically important in PAH.

Keywords:

• ambrisentan
• bosentan
• endothelin receptor antagonists
• pulmonary arterial hypertension
• sitaxsentan

Introduction to newer approaches to management of pulmonary arterial hypertension

Since the discovery of endothelin-1 (ET-1) in the late 1980s, scientific research has established that excess synthesis of ET-1 is an important factor in the pathogenesis of pulmonary arterial hypertension (PAH). This led to the development of a class of drugs called endothelin receptor antagonists (ERAs). On the basis of a series of randomized controlled clinical trials, bosentan, ambrisentan, and sitaxsentan are licensed in the United States and/or Europe as monotherapy for patients with PAH in Group 1 World Health Organization (WHO) classification (Table 1).

Table 1 Pulmonary hypertension, Group I World Health Organization Classification (after Venice 2003)

Group Ia
Idiopathic IPAH (IPAH)
Familial PAH (FPAH)
Related to
Connective tissue diseases HIV Portal hypertensionb Anorexigens Congenital heart diseases
Primary pulmonary hypertension of the newborn (PPHN)
PAH with venule/capillary involvement (pulmonary veno-occlusive disease, PVOD)
Others: Glycogen storage disease, Gaucher’s, hemoglobinopathies (ie, sickle cell), hereditary hemorrhagic telagiectasia (HHT)

a Endothelin receptor anagonists have not been studied formally in portal hypertension, PPHN, PVOD and other types of group I PAH.

b Given the potential for liver toxicity, caution is advised when using these agents in patients with portal hypertension due to end stage liver disease.

ET-1 is a potent vasoconstrictor that is overexpressed in the plasma and the lungs of patients with PAH, especially in the remodeled precapillary pulmonary microvasculature which is the site of increased pulmonary vascular resistance in PAH (Giaid et al 1993). Studies suggest that dysregulated proliferation and abnormal apoptosis of endothelial cells are integral to the development of PAH (Voelkel et al 1998; Humbert et al 2004; Michelakis 2006). Indeed, scientific work shows that excess ET-1 levels not only cause significant vasoconstriction, but also result in both the abnormal growth pattern of endothelial cells, smooth muscle cells, fibroblasts, and pericytes and inhibit apoptosis of both smooth muscle cells and endothelial cells (Jankov et al 2006; Shichiri et al 1997). These events may contribute to the ongoing vascular remodeling seen in PAH.

Review of pharmacology, mode of action, pharmacokinetics of endothelin receptor antagonists with specific reference to differential effects of the various agents

Mode of action

ET-1 acts on two G protein-coupled receptors termed ET_A and ET_B (Arai et al 1990; Sakurai et al 1990). ET_A receptors are abundant on smooth muscle, pericytes, and fibroblasts and their activation by ET-1 results in vasoconstriction and proliferation in vitro (Evans et al 1999). ET_B receptors are present on endothelial cells as well as pulmonary artery smooth muscle cells. Distal lung microvasculature have a greater proportion of ET_B receptors and the receptor density in distal arteries is twofold greater in pulmonary hypertensive patients compared to normal human pulmonary arteries (Davie et al 2002a). ET-1 activates ET_B receptors at low doses, whilst at higher doses ET_A receptors are activated. Both ET-1 receptors mediate smooth muscle cell contraction (McCulloch et al 1996) and proliferation (Davie et al 2002b). In addition, stimulation of ET_B receptors results in the release of vasodilators and antiproliferative molecules such as prostacyclin and nitric oxide from the endothelium (de Nucci et al 1988), and results in ET-1 clearance from circulation (Dupuis et al 1996a, b). Further animal work has shown that by blocking ET_B receptors, ET-1 vasoconstrictive activity is enhanced (via the ET_A receptor), due to inhibition of the transient ET_B induced vasodilatation and ET-1 clearance. In other animal models of PAH, ET_A receptor blockade decreased the degree of pulmonary hypertension by 25% with no effect from the ET_B receptor blockade (Black et al 2003). Conversely, other studies showed that combined ET_A and ET_B receptor blockade inhibited ET-1 induced vasoconstriction more effectively than the ET_A blocker alone (Sato et al 1995) and in monocrotaline-induced pulmonary hypertension, dual ETA/B blockade produced better survival than selective ET_A blockade (Jasmin et al 2001). Nevertheless, selectively blocking the ET_A receptors and preserving the vasodilatory and clearance function of the ET_B receptors may be of benefit in patients where excess synthesis of ET-1 rather than reduced clearance is resulting in excess pulmonary vascular constriction (Langleben et al 2006). However, because there are differences between experimental and clinical forms of PAH and clinical studies have not been performed to address if there are clinically significant differences between dual and selective ERAs, the therapeutic superiority of selective versus combined ET receptor blockade remains unanswered and has been subject to great debate.

Two ERAs are on the US market: bosentan (Tracleer^®, Actelion Pharmaceuticals Ltd, Allschwil, Switzerland) and ambrisentan (Letairis^®, Gilead Sciences, Foster City, CA, USA). Sitaxsentan (Thelin^®, Encysive Pharmaceuticals, Houston, Texas, USA) is approved in Canada, Europe, and Australia. Bosentan inhibits both the ET_A and ET_B receptors (ET_A: ET_B 20:1) while ambrisentan (ET_A: ET_B 260:1) and sitaxsentan (ET_A: ET_B 6500:1) are selective for the ET_A receptor (Battistini et al 2006).

Pharmacokinetics

Bosentan is dosed as 62.5 mg twice a day the first 4 weeks and 125 mg twice a day thereafter (Dingemanse et al 2002a), ambrisentan is dosed 5 or 10 mg once a day (Galie et al 2005; Prod Info Letairis^® oral tablets 2007), and sitaxsentan is dosed 100 mg once a day (Barst et al 2006). Unlike bosentan and sitaxsentan, ambrisentan has a propanoic acid structure (Battistini et al 2006). This difference may account for the lack of liver toxicity and/or lack of dug-drug interaction reported with ambrisentan.

Drug concentration levels

Bosentan administered orally reaches peak concentration within 3 to 5 hours and steady state by 3–5 days (Dingemanse et al 2002a). Concomitant administration of bosentan and inhibitors of CYP3A4 can increase the peak plasma concentration by more than 2-fold (van Giersbergen et al 2002a). Oral ambrisentan reaches peak concentration within 2 hours after administration (Prod Info Letairis^® oral tablets 2007) and oral sitaxsentan reaches peak concentration within 1 to 3 hours (Wu et al 2004b).

Absorption

Bosentan’s bioavailability is thought to be 50% and is similar for 125 mg tablet or with two 62.5 mg tablets (Dingemanse et al 2002b). Ambrisentan is rapidly absorbed after oral administration but the bioavailability is unknown. Food has no effect on the bioavailability of ambrisentan or bosentan. Sitaxsentan’s oral bioavailability is greater than 90% (Widlitz et al 2005).

Distribution

All three ERAs are highly bound to plasma proteins (Wu et al 2004b; Dingemanse et al 2002a; Prod Info Letairis^® oral tablets 2007).

Metabolism

Bosentan is extensively metabolized by the liver (Weber et al 1996b). Ambrisentan is a strong inhibitor of P-glycoprotein, organic anion transport protein, cytochrome P450 and uridine 5 diphosphate glucuronosyltransferases UGTs (Prod Info Letairis^® oral tablets 2007). Sitaxsentan is a moderate inhibitor of liver cytochrome CYP2C9 (Widlitz et al 2005). It displays nonlinear metabolism at a dose of 300 mg, while 100 mg dose has linear metabolism (Barst et al 2004).

Drug-drug interaction

All three drugs are contraindicated in conjunction with cyclosporine A and glyburide (van Giersbergen et al 2002b; Treiber et al 2007). Bosentan induces warfarin metabolism and requires an increase in the warfarin dose (Murphey and Hood 2003), while sitaxsentan decreases warfarin metabolism, requiring a drop in the warfarin dose (Barst et al 2006). There are no known interactions between ambrisentan and warfarin.

Excretion

The elimination of bosentan is primarily through the biliary system with only 3% or less excreted through the kidneys (Weber et al 1996a). The elimination of ambrisentan is mainly by nonrenal pathways and the relative contributions of metabolism and biliary elimination have not been characterized (Prod Info Letairis^® oral tablets 2007). Fifty to 60% of sitaxsentan is eliminated via the urine while the rest is eliminated via the feces (Wu et al 2004b).

Elimination half-life

The half-life of bosentan is 5–8 hours (Weber et al 1996a). The half-life of ambrisentan is 9–15 hours (Prod Info Letairis^® oral tablets 2007). The half-life of sitaxsentan is 6–7 hours (Wu et al 2004a).

Studies of the various endothelin antagonists in PAH

Table 2 summarizes the studies discussed in this review.

Table 2 Major trials using endothelin receptor antagonists in pulmonary arterial hypertension

Reference (Sponsor)	Trial design	Drug (dosage)	Duration (weeks) (study name)	Populations	Functional class	Na	Primary endpoint	Primary effect	Secondary endpoints achieved	Liver enzymesb
Channick 2001 (Actelion)	DBPC	Bosentan (62.5 mg bid × 4 weeks, 125 mg bid) or placebo	12 weeks	IPAHc, Scleroderma	III	32	6MWDd	Yes	Functional Class Clinical Worsening Hemodynamics Borg Scale	–
Rubin LJ 2002 (Actelion)	DBPC	Bosentan 125 mg, 250 mg bid or placebo	16 weeks| (BREATHE-1)	IPAH, CTDe	III, IV	213	6MWD	Yes	Functional Class Clinical Worsening Borg Scale	–
Galie N 2003 (Actelion)	–	Bosentan 125 mg, 250 mg bid or placebo	16 weeks (BREATHE-1 Echo substudy)	IPAH, CTD	III, IV	56	Echo and Doppler parameters	Yes	Echo parameters	–
Sitbon 2003 (Actelion)	–	Bosentan 125 mg bid	>1 year	IPAH, Scleroderma	III	29	Safety	Yes	Functional Class Hemodynamics 6MWD	–
McLaughlin 2005 (Actelion)	–	Bosentan 125 mg bid	>2 year	IPAH, CTD	III, IV	169	Survival	Yes	Survival Hemodynamics	14.9%
Barst 2003 (Actelion)	–	Bosentan weight based	12 weeks BREATHE-3	Pediatric IPAH CHD	II, III	19	Hemodynamics	Yes	Functional Class	–
Sitbon 2004 (Actelion)	–	Bosentan 125 mg bid	16 weeks BREATHE-4	HIV	III, IV	16	6MWD	Yes	Functional Class Hemodynamics Quality of Life Echo parameters	–
Galie N 2006 (Actelion)	DBPC	Bosentan 125 mg bid	16 weeks BREATHE-5	Eisenmenger	III	37	Pulmonary vascular resistance	Yes	Functional Class Hemodynamics	–
Barst 2004 (ICOS/Encysive)	DBPC	Sitaxsentan 100–300 mg qd	12 weeks STRIDE-1	IPAH, CTD, CHDg	III	118	6MWD	Yes	Functional Class Hemodynamics	0% at 100 mg dose
Langleben 2004 (Encysive)	–	Sitaxsentan 100 to 300 mg qd	1 year STRIDE-1XC	IPAH, CTD, CHD	II, III	11	Safety	Yes	Functional Class Hemodynamics	–
Barst 2006 (Encysive)	DBPC*	Sitaxsentan 50 mg or 100 mg qd or bosentan 125 mg bid	18 weeks STRIDE-2	IPAH, CTD, CHD	II, III, IV	183	Change in peak VO2	Yes for 300 mg dose	Functional Class 6MWD	3% sitaxsentan 100 mg, 11% bosentan
Pulido 2006 (Encysive)	DBPC	Sitaxsentan 50 mg or 100 mg qd	18 weeks STRIDE-4	IPAH, CTD, CHD	II, III, IV	64	6MWD	Yes in 100 mg dose	Functional Class Borg Scale	3%
Benza 2006 (Encysive)	–	Sitaxsentan 100 mg QD or bosentan 125 mg bid	52 weeks STRIDE-2X	IPAH, CTD, CHD	II, III	Sitaxsentan 145, bosentan 84	Safety	Yes	Functional Class Clinical Worsening Survival	4% sitaxsentan 14% bosentan
Benza 2005 (Encysive)	–	Sitaxsentan 100 mg qd	12 weeks STRIDE-6	IPAH, CTD, CHD	II, III	35	Safety	Yes	Borg Scale
Galie 2005 (Myogen)	DB	Ambrisentan 1, 2.5, 5, or 10 mg QD	24 weeks	IPAH, CTD, HIV, anorexigen	II, III	64	6MWD	Yes	Functional Class Hemodynamics Borg Scale	3%
Oudiz 2006 (Myogen)	DBPC	Ambrisentan 5 mg or 10 g qd	12 weeks ARIES-1	IPAH, CTD, HIV, anorexigen	II, III	124	6MWD	Yes		0%
Olschewski 2006 (Myogen)	DBPC	Ambrisentan 2.5 mg or 5 mg qd	12 weeks ARIES-2	IPAH, CTD, HIV, anorexigen	II, III, IV	124	6MWD	Yes	Functional Class Clinical Worsening Borg Scale Quality of Life	0%

a N = number of subjects enrolled.

b Abnormal liver function test were considered above 3x upper limit of normal.

c IPAH = idiopathic pulmonary arterial hypertension.

d 6MWD = 6-minute walk distance.

e CTD = connective tissue disease.

f CHD = congenital heart disease.

g DB = double-blind.

h PC = placebo-controlled.

i DBPC* = double-blind only for sitaxsentan arms.

Bosentan

A pilot study of 7 patients with PAH showed that an infusion of high doses of bosentan acutely lowered both pulmonary and systemic vascular resistances (Williamson et al 2000). Several of the patients died or suffered clinical deterioration during the second phase of the study, which may have been related to their poor clinical status. ET-1 levels were elevated, consistent with a concomitant blockade of the endothelial ET_B receptor.

These data led to the first randomized, double-blind, placebo-controlled 12 week trial to evaluate the clinical effects of bosentan as a long-term oral treatment (Channick et al 2001). Oral bosentan (62.5 mg twice a day for 4 weeks, then 125 mg twice daily) improved exercise capacity (measured by 6-minute walk distance), pulmonary vascular resistance, and WHO functional class in patients with idiopathic PAH or PAH related to scleroderma. All patients had WHO functional class III at baseline. In both groups, more patients had idiopathic PAH than PAH related to scleroderma. The main difference between the groups was the slightly longer duration of disease before the diagnosis in patients assigned placebo than those assigned bosentan. The incidence of hepatotoxicity in bosentan treated patients was 10% and resolved with discontinuation of the drug.

A 16 week, double blind, placebo-controlled study, the Bosentan Randomized Trial of Endothelin Antagonist Therapy (BREATHE-1) showed that patients treated with oral bosentan (62.5 mg twice a day for 4 weeks, then either 125 mg or 250 mg twice daily) had improved exercise capacity as measured by the 6-minute walk distance, and functional class, and it delayed time to clinical worsening compared with placebo (Rubin et al 2002). Most patients were in functional class III, with a few in class IV. In both groups, more patients had idiopathic PAH than PAH associated with connective tissue disorders. Subgroup analysis suggested that bosentan improves the walking distance from baseline in patients with idiopathic PAH (46 m increase as opposed to a 5 m decline in the placebo group) while bosentan appeared to prevent deterioration in the walking distance only among patients with scleroderma. Abnormal hepatic function was found to be dose dependent. Increases in hepatic transaminase levels to more than 8 times the upper limit of normal was seen only in the bosentan groups; 3% in the group receiving 125 mg twice daily and 7% in the group receiving 250 mg twice daily, with the two doses exhibiting similar efficacy on the end points. A substudy subsequently showed that bosentan improved right ventricular size and systolic function as well as left ventricular filling according to echocardiography (Galie et al 2003). BREATHE-1 resulted in approval of the first oral therapy for PAH. The recommended dose for bosentan in adults is 62.5 mg twice daily for 4 weeks and then increase to 125 mg twice daily long-term so long as there is no hepatotoxicity.

A 1-year follow-up open label study to determine the long-term benefit of bosentan and in particular whether tolerance develops over time was assessed in patients with class III functional status at baseline (Sitbon et al 2003). Six-minute walk distances, pulmonary hemodynamics, and functional status were sustained for many patients. By 1 year, only 55% of patients were still in class III, while 40% had improved to class II. Only 4 of 29 patients required up-titration to 250 mg twice daily to maintain a favorable clinical status. The incidence of hepatotoxicity was 9.7%, but was not severe enough to warrant discontinuation of the drug.

Two-year survival rates for bosentan in 2 observational cohorts (McLaughlin et al 2005; Sitbon et al 2005) were similar (89% and 91% respectively) and they are better than the predicted survival rate of 57% based on the equation formulated by the NIH registry. Factors that predicted a worse outcome included WHO functional class IV and 6-minute walk distance below the median (358 m) at baseline (McLaughlin et al 2005). However, the lack of a control group and the comparison with a historical group, which probably had more severe patients, may bias the results toward bosentan therapy and limit our ability to determine that bosentan improves survival.

Five additional studies evaluated the efficacy of bosentan for patients with pulmonary hypertension associated with other diseases and age groups. BREATHE-3 showed improved pulmonary hemodynamics in 19 pediatric patients with PAH related to congenital heart disease after a 12-week open-label weight adjusted bosentan therapy trial (Barst et al 2003). Approximately 50% of the patients were on concomitant epoprostenol therapy. In BREATHE-4, patients with HIV-related PAH showed improved 6-minute walk distance, functional class, hemodynamics, Doppler echocardiographic indices, and quality of life in a 16 week open label study of bosentan therapy (Sitbon et al 2004). There was a 9% incidence of hepatotoxcity and there were no adverse interactions related to antiretroviral medications. BREATHE-5 evaluated bosentan therapy in 54 patients with class III PAH due to congenital heart disease and Eisenmenger’s syndrome in a randomized double blinded placebo controlled study over 16 weeks (Galie et al 2006). Bosentan therapy improved hemodynamics and exercise capacity in these patients.

Bosentan has also shown to improve functional capacity and symptoms in two prospective studies enrolling patients with pulmonary hypertension secondary to inoperable thromboembolic disease (Bonderman et al 2005; Hoeper et al 2005).

Ambrisentan

Ambrisentan is an endothelin receptor antagonist that is selective for ET_A with a bioavailability and half-life (9–15 hours) that allows once a day dosing. A 12-week blinded to dose but without a placebo armed study was performed to determine the efficacy and safety of 4 doses in patients with PAH (Galie et al 2005). Patients at all doses (1, 2.5, 5, or 10 mg daily) had improved 6-minute walk distance, functional class, Borg scale, and hemodynamics as compared with baseline. Adverse events were mild and unrelated to dose, including the 3% incidence of elevated hepatic transaminases of >3x the upper limit of normal. Subgroup analysis showed that patients with idiopathic PAH appeared to have a dose-response relationship for the 6-minute walk distance.

In a phase III, randomized, double-blinded, placebo-controlled, multicenter, efficacy study of ambrisentan in subjects with PAH (ARIES-1), 202 patients were randomly assigned to 5 mg or 10 mg of ambrisentan or placebo once daily. There were significant improvements in 6-minute walk distance, functional class, Borg dyspnea score, and quality life score (Oudiz et al 2006). In ARIES-2, patients with PAH were randomly assigned to placebo, ambrisentan 2.5 mg, or ambrisentan 5 mg once daily over 12 weeks (Olschewski et al 2006). There were also significant improvements in 6-minute walk distance and a delay to clinical worsening. No significant adverse events occurred including no interaction with warfarin therapy, nor did any patient develop elevated hepatic transaminases above 3x the upper limit of normal.

Sitaxsentan

Sitaxsentan was evaluated in an open-label pilot study to determine the safety and efficacy of 12 weeks of therapy on exercise capacity and hemodynamics in children and adults with idiopathic PAH, and associated diseases of PAH such as congenital heart disease and collagen vascular disease (Barst et al 2002). The study demonstrated significant increases in 6-minute walk distances and improved hemodynamics in patients who had functional class II, III, and IV PAH at doses between 100 to 500 mg twice daily. In the extension phase, 2 patients had severe hepatitis resulting in 1 death despite stopping the drug. The hepatic toxicity is related to the non-linear pharmacokinetics of sitaxsentan at high doses and thus subsequent studies have used doses up to 300 mg once a day.

This lead to STRIDE-1, the first randomized double-blinded placebo-controlled trial using lower doses of Sitaxsentan (100 mg and 300 mg) and administering it only once daily for 12 weeks. STRIDE-1 included patients with idiopathic PAH and associated PAH diseases such as congenital heart disease and connective tissue disease and had no ceiling on the 6-minute walk distance, allowing patients with less severe PAH to be enrolled. STRIDE-1’s primary endpoint was peak oxygen consumption on the cardiopulmonary exercise testing and the results showed only an improvement in the percent of predicted peak oxygen consumption in the 300 mg group compared with placebo. This index was subsequently found to have interhospital variability, and thus its use in multi-center trials is questionable (Oudiz et al 2006). However, the secondary endpoints 6-minute walk distance, WHO functional class, and pulmonary hemodynamics all improved significantly at both 100 mg and 300 mg doses. The most frequently reported clinical adverse events with sitaxsentan treatment were headache, peripheral edema, nausea, nasal congestion, and dizziness. The most frequently reported laboratory adverse event was increased international normalized ratio or prothrombin time, related to the effect of sitaxsentan on inhibition of CYP2C9 P450 enzyme, the principal enzyme involved in the metabolism of warfarin (Barst et al 2002). The incidence of elevated hepatic transaminases above three times the upper limit of normal was 0% at 100 mg dose and 10% at the 300 mg dose. However, by 26 weeks in the extension study (STRIDE-1X) there was a 5% incidence of elevated transaminases with the 100 mg dose and 21% incidence with the 300 mg dose.

A post-hoc subgroup analysis was performed on 42 patients who had PAH associated with connective tissue disease out of the 178 patients enrolled in STRIDE-1 (Girgis et al 2007). Those patients treated with 100 mg or 300 mg of sitaxsentan had significant improvements in their 6-minute walk distance (a mean of 58 m placebo-subtracted treatment effect, p = 0.027), quality of life, and hemodynamics. Sitaxsentan appears to be tolerated well with only two patients that developed elevation of hepatic transaminases. A 1-year follow-up STRIDE-1 study, revealed that eleven PAH patients tolerated 100 mg of sitaxsentan daily well with sustained improvement in functional class, pulmonary vascular resistance, and cardiac output (Langleben et al 2004).

An 18-week, double-blinded study compared sitaxsentan at 50 mg or 100 mg to placebo or open label bosentan (62.5 mg twice daily for 4 weeks and then 125 mg twice daily) (STRIDE-2) (Barst et al 2006). The 100 mg sitaxsentan and the bosentan arms, but not the 50 mg sitaxsentan arm, showed significant and similar improvements in 6-minute walk distance and functional class. The incidence of elevated hepatic transaminases (>3x the upper limit of normal) was 3% for 100 mg sitaxsentan, 5% for 50 mg sitaxsentan, 11% for bosentan, and 6% for placebo. STRIDE-4 is another study comparing 50 mg with 100 mg dose of sitaxsentan and placebo in Latin America, Poland, and Spain, which enrolled mostly patients in NYHA functional class II (Pulido et al 2006). The 100 mg dose improved 6-minute walk distance, functional class, Borg dyspnea scale, and time to clinical worsening, while the 50 mg dose did not. Interestingly, however, the placebo groups also had a significant improvement in 6-minute walk distance by 34 m. This placebo improvement effect is thought to be due to the perception of improved medical care after enrollment among patients who might not otherwise have had access to that level of care. Patients tolerated sitaxsentan well with only 1 patient in each group developing elevated hepatic transaminases. In the STRIDE-2X extension open-label study (Benza et al 2006), patients who initially received 50 mg of sitaxsentan were given 100 mg and those who were on placebo were either assigned to 100 mg of sitaxsentan daily or 125 mg twice daily of bosentan, while those patients on either 100 mg of sitaxsentan or 125 mg of bosentan remained on the same therapy. At one year time to clinical worsening and liver function test abnormalities were better in the sitaxsentan group. In addition, the 1-year risk of discontinuation from monotherapy was 25% for sitaxsentan versus 42% for bosentan (p = 0.003). However, these interpretations should be made with caution, as the study was not powered to detect differences between treatments. STRIDE-3 is a long-term safety trial with over 800 patients enrolled and the results have not been reported yet.

STRIDE-6 (Benza et al 2007) studied the safety and efficacy of sitaxsentan in patients discontinuing bosentan due to hepatotoxicity or inadequate efficacy. After 12 weeks, only 1 of the 12 patients who had previously experienced hepatotoxicity on bosentan developed elevated liver enzymes with sitaxsentan. Five of the 15 (33%) who discontinued bosentan because of lack of efficiency had an improvement in 6-minute walk distance of more than 115% while taking sitaxsentan. No long-term data are yet available for this group.

Combination therapy with endothelin receptor antagonists

Patients’ quality of life and survival with PAH still remain poor despite the tremendous medical advances with monotherapy. This has led to attempts to combine different classes of therapies that have different actions such as ERAs with phosphodiesterase-5 inhibitors (eg, sildenafil) or prostanoids (eg, epoprostonol, trepostinil, iloprost, or beraprost). Unfortunately, studies to ascertain what combinations are beneficial and which have potential for adverse drug-drug interactions have not been conducted. A pilot pharmacologic study assessed the combination of bosentan with sildenafil (a phosphodiesterase-5 inhibitor approved for the treatment of PAH) in 10 patients with PAH (Paul et al 2005). Bosentan was given 62.5 mg twice daily for 1 month, then 125 mg twice daily for a second month. Sildenafil 100 mg was given before the first bosentan dose and at the end of each month of bosentan treatment. Treatment with bosentan 62.5 mg twice daily was associated with a 2-fold increase in sildenafil clearance. Increasing the dose of bosentan to 125 mg twice daily led to a further increase in sildenafil oral clearance, demonstrating that bosentan decreases the plasma concentration of sildenafil. Preliminary results from the EARLY trial (Rubin et al 2007) looking at 29 patients with mild PAH (WHO functional class II) on sildenafil in whom bosentan was added showed that the addition of bosentan decreased pulmonary vascular resistance by 20% and a delay to clinical worsening, although there was no improvement in the 6-minute walk distance. A recent study evaluated whether combination therapy after failure of bosentan monotherapy, particularly in patients with scleroderma-associated PAH (PAH-SSD) was effective. Addition of sildenafil improved New York Heart Association class and 6-min walk distance in idiopathic PAH patients but failed to improve either parameter in PAH-SSD patients (Mathai et al 2007). Another study evaluated the addition of bosentan in PAH patients already on either inhaled iloprost or oral beraprost, two prostanoids. The addition of bosentan in an open-label fashion resulted in improved 6-minute walk distances after 3 months of combined therapy and the therapy was well tolerated (Hoeper et al 2003). In a 16-week BREATHE-2 study, patients already receiving intravenous epoprostenol had either bosentan or placebo added (Humbert et al 2004). The results were not as promising. Hemodynamics improved but not significantly and there was no improvement in functional class or exercise capacity. This study, however, was not powered to detect differences in efficacy. More importantly, there were serious adverse events, including death in the group receiving epoprostenol and bosentan. In the recent (STEP) combination trial, inhaled iloprost or placebo was added to patients receiving bosentan therapy (McLaughlin et al 2006). By week 12, patients receiving bosentan and iloprost had improved their New York Heart Association status by one class and had a delay to clinical worsening compared with those patients who were on bosentan and placebo. Also of note is that the post-inhalation iloprost-bosentan group had improved their pulmonary hemodynamics. A safety and efficacy study (Hoeper et al 2006) of inhaled iloprost in those patients already treated with bosentan was terminated early after a futility analysis revealed that the primary endpoint, change in 6-minute walking distance, failed to show a positive effect of adding inhaled iloprost. Further studies involving larger sample sizes and long-term follow-up are needed to determine the efficacy of adding inhaled iloprost to bosentan in patients with idiopathic PAH.

Transitioning from other PAH therapies to endothelin receptor antagonists

Transitioning patients onto oral therapy from parental forms of therapy is an attractive goal for our patients with PAH. The first study reported 4 patients with normal hemodynamics on epoprostenol who were successfully transitioned to oral bosentan (Kim et al 2003). A subsequent study evaluated three children who had normal hemodynamics on epoprostenol and showed that these children after having been switched to bosentan remained stable for a full 1-year study period (Ivy et al 2004). However, in subsequent studies that evaluated patients on parental prostanoids that did not normalize their pulmonary hemodynamics prior to adding bosentan, only 75% transitioned successfully to bosentan and of those that transitioned more than 60% on bosentan therapy alone deteriorated within 3–16 months after the prostanoids were stopped (Suleman and Frost 2004; Steiner et al 2006). Given the high percentage of patients who deteriorate after they are transitioned to oral ERA therapy and the lack of randomized multicenter controlled trials evaluating the safety and efficacy of transitioning patients, we recommend only transitioning patients from prostaglandins to bosentan in those who have normalized their pulmonary hemodynamics and are under close observation.

Differential safety and tolerability

Both bosentan and sitaxsentan have been reported to cause hepatic toxicity, while ambrisentan seems to have no effects on the liver. In a post-market analysis of 4,994 patients on bosentan followed up prospectively for 30 months, the incidence of liver function test elevation above 3 times the upper limit of normal was 7.6%, with an annual rate of 10.1% and a discontinuation rate of 3.7% (Humbert et al 2007). From the STRIDE trials, the incidence of liver abnormalities with sitaxsentan is approximately 5% with the 100 mg daily, while ambrisentan does not seem to affect liver function. Nevertheless, all three compounds require monthly monitoring of liver function tests. ERAs are potent teratogens and contraception is required for women with childbearing potential (Spence et al 1999).

Patient-focused perspectives such as quality of life, patient satisfaction, tolerability, adherence, and uptake

All pivotal studies have shown improvement in functional capacity with ERAs compared with placebo. Only two studies have formerly evaluated patients’ quality of life using the SF-36 questionnaire; BREATHE-4 (bosentan in HIV) and ARIES-2 (ambrisentan), both of which showed significant improvement. However, the SF-36 questionnaire which is used in patients with chronic lung disease has not been validated for PAH. Nevertheless, a combination of improvement in functional capacity, coupled with the ease of administration, is a major advantage of this class of therapies. Since placebo control randomized studies assessing survival in PAH patients treated with ERAs have not been performed, it is difficult to ascertain whether these drugs affect survival.

Conclusions, optimizing selection, and use in therapy

Since the discovery of ET-1 in 1988, and its pivotal role in the pathogenesis of PAH, endothelin receptor antagonist therapies have emerged, improving the lives of many patients with PAH. For patients with Group 1 PAH with a negative vasoreactivity test and NYHA class II–IV, non-selective endothelin receptor antagonist bosentan improves hemodynamics, exercise capacity and delays clinical worsening. There is published experience with bosentan in various subgroups of PAH patients. In addition, selective ET_A antagonists such as ambrisentan and sitaxsentan also improve exercise tolerance, functional class, hemodynamics, and quality of life. To date, we lack comparative clinical studies to evaluate whether selective inhibition has a clinical advantage over nonselective inhibition. From the current data we know that the safety profiles and the drug-drug interactions are different for the different ERAs. Although monthly liver test monitoring is required for all three drugs, ambrisentan has the least liver toxicity. Concomitant warfarin therapy with sitaxsentan will require the warfarin dose to be reduced, while with bosentan, the warfarin dose must be increased, and with ambrisentan there is no need to alter the warfarin dose.

Many questions remain unanswered and deserve further study, such as understanding the pharmacogenomics of responders and non-responders to therapy, a better understanding of the importance of selectivity versus nonselectivity, the need for robust survival data, and determining the effective combination therapy. Limited experience suggests that bosentan can be used safely with epoprostenol or treprostinil. The benefits of bosentan plus sildenafil are best described in patients with idiopathic PAH, and less so in scleroderma-associated PAH. The effect of combining bosentan with iloprost is less clear. Larger trials are underway to investigate the role of combination oral therapies. Despite the ongoing unanswered questions, the rapid translation of basic science into applicable and efficacious therapies for patients with PAH has had a tremendous impact in caring for these patients.

Disclosures

Dr Preston has received grants from and is on the speakers’ bureau for Actelion and Gilead Pharmaceuticals.