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Abstract:
Two morphologically distinct species, Brigantedinium majusculum and Trinovantedinium applanatum, have been suggested as a cyst stage of Protoperidinium pentagonum; however, the cyst–theca relationships are still virtually undefined. In this study, we re-examined the motile stage of B. majusculum via thecal plate analysis together with a molecular phylogenetic analysis based on partial large-subunit ribosomal DNA gene sequences. The morphological features of motile cells germinated from B. majusculum were identical to those of Pr. sinuosum, and the molecular phylogenetic analysis showed that the germinated cells and B. majusculum are closely related to Pr. pentagonum and Pr. latissimum as a sister group. Additionally, high sequence divergences were observed between the germinated cells and B. majusculum as well as Pr. pentagonum and Pr. latissimum (18.0–19.5% according to P-values and 20.7–22.9% according to Kimura two-parameter values). On the basis of these results, our study indicates that B. majusculum should be accepted as the cyst of Pr. sinuosum.
ACKNOWLEDGEMENTS
This work was supported by grants from Marine Biotechnology Program, and Construction test, evaluation, and certification systems for US Coast Guard phase II standard (PM58580) funded by Ministry of Oceans and Fisheries, Korea.
SUPPLEMENTARY DATA
Supplementary data associated with this article can be found online at http://dx.doi.org/10.2216/15-47.1.s1.