Association of Paraoxonase-1 L55M and Q192R Polymorphisms with Pcos Risk and Potential Risk Factors for Atherosclerosis

Abstract

Aim: To examine the PON1-L55M and -Q192R polymorphisms for polycystic ovary syndrome (PCOS) risk in relation with atherosclerosis risk markers. Methods: Blood samples were collected from 203 women (PCOS [n = 151], control [n = 52]). Genomic DNA was extracted and RFLP method was performed following the amplifications of the target regions. Results: Individuals with 192QR/192RR genotypes had a 2.5-fold increased risk of representing PCOS compared with the individuals with 192QQ genotype. Q192R was more strongly associated with PCOS than previously suggested atherosclerosis risk markers. Q192R status and body mass index values in combination were established to be a significant predictor of PCOS (AUC: 0.655, p = 0.001). Conclusion: This is one of the first studies suggesting the use of combination biomarkers to better predict the risk of developing PCOS.

Keywords:

• atherosclerosis risk factors
• body mass index
• insulin resistance
• metabolic syndrome
• paraoxonase 1 gene
• polycystic ovary syndrome
• predictive marker
• single nucleotide polymorphism

Acknowledgements

This work was supported by Recep Tayyip Erdogan University, Scientific Research Projects Unit (grant number 2013.106.01.3).

Financial & competing interests disclosure

This work was supported by Recep Tayyip Erdogan University, Scientific Research Projects Unit (grant number 2013.106.01.3). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

This study was approved by the Ethics Committee of Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey. All of the participants were informed of the aim and the procedures in detail and asked to give written consent. All subjects were recruited from the Recep Tayyip Erdogan University Hospital, Rize.