Abstract
Aim: Change at a molecular level should occur before histopathological impairments. We refer to these changes as ‘prechronic disease state.’ Methods: Two blood samples were taken 1 day apart, 1 month apart and 2 years apart to determine the contribution of genetic and biological variations. Results: The 95% CI calculated quantitatively by the variability of genetic variation was very similar to the reference interval provided by the laboratory for most indicators, indicating that pathological variability had occurred without consideration of biological variation if an indicator's value lies outside of the reference interval. Conclusion: The physiological or predisease state can be distinguished directly using the laboratory reference interval.
Acknowledgements
The authors thank L Qigui, a statistician at their university, for checking the statistical analysis of their study.
Financial & competing interests disclosure
This work was supported by the National Natural Science Foundation of China (grant number 30770724), Special Grant for Scientific and Technological Innovation of Dalian (2018J12SN084) and the Special Grant for Translational Medicine, Dalian Medical University (grant number No. 2015008). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
No writing assistance was utilized in the production of this manuscript.
Ethical conduct of research
All of the experiments were conducted in accordance with the Declaration of Helsinki, and the study was approved by the Institutional Ethics Committee of Dalian Medical University without informed consent due to the observational nature of the study.