![Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5925/TOC-Subject-Sample-2021-Bioscience.jpg"})
Abstract
Aim: We aimed to explore the roles of circular RNAs (circRNAs) in extracellular vesicles (EVs) isolated from serum of patients with endometrial cancer. Materials & methods: EVs were isolated from serum samples of three patients with stage III adenocarcinoma aged 50–60 years and three matched healthy controls. RNA was extracted from the EVs and analyzed using RNA-seq technique. Results: We got 209 upregulated circRNAs and 66 downregulated circRNAs. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that the differentially expressed circRNAs were enriched in five pathways. The expression level of hsa_circ_0109046 and hsa_circ_0002577 was confirmed using real-time quantitative PCR. Conclusion: We identified 275 differentially expressed circRNAs and the expression level of two circRNAs was confirmed using real-time quantitative PCR.
Financial & competing interests disclosure
This study was funded by the National Natural Science Foundation of China (Grant Number 81602551). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
No writing assistance was utilized in the production of this manuscript.
Ethical conduct of research
The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.
Supplementary data
To view the supplementary data that accompany this paper please visit the journal website at:www.tandfonline.com/doi/full/10.2217/epi-2017-0109