TCONS_00483150 as a Novel Diagnostic Biomarker of Systemic Iupus Erythematosus

Abstract

Aim: We aimed to identify differentially expressed Long noncoding RNAs (lncRNAs) and explore their functional roles in systemic lupus erythematosus (SLE). Materials & methods: We identified dysregulated lncRNAs and investigated their prognostic values and potential functions using MiRTarget2, catRAPID omics and Bedtools/blast/Pearson analyses. Results: Among the 143 differentially expressed lncRNAs, TCONS_00483150 could be used to distinguish patients with SLE from healthy controls and those with rheumatoid arthritis and patients with active/stable SLE from healthy controls. TCONS_00483150 was significantly correlated with anti-Rib-P antibody positivity and low C3 levels; TCONS_00483150 dysregulation might contribute to the metabolism of RNA and proteins in SLE patients. Conclusion: Overall, our findings offer a transcriptome-wide overview of aberrantly expressed lncRNAs in patients with SLE and highlight TCONS_00483150 as a potential novel diagnostic biomarker.

Keywords::

• biomarker
• long noncoding RNA
• RNA-sequencing
• systemic lupus erythematosus

Supplementary data

To view the supplementary data that accompany this article please visit the journal website at: www.tandfonline.com/doi/suppl/10.2217/epi-2019-0265

Acknowledgments

We thank the patients and healthy volunteers who participated in this study.

Financial & competing interests disclosure

This study was supported by Medical Health Science and Technology Project of Zhejiang Provincial Health Commission (no. 2019KY453), by the Ningbo Natural Science Foundation (grant number 2015A610179), by Zhejiang Science and Technology Bureau (no. 2012C33126), the Zhejiang Provincial Natural Science Foundation of China (no. LY20H100003 and LY15H100004) and the Wenzhou Municipal Science and Technology Bureau (no. 2014Y0309). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

The research protocol was approved by the Medical Ethical Committees of the First/Second Affiliated Hospitals of Wenzhou Medical University and Renji Hospital of Shanghai Jiaotong University School of Medicine. All subjects who participated in this research provided written informed consent.

Data sharing statement

All data generated or analyzed during this study are included in this published article and its supplementary information files.

Additional information

Funding

This study was supported by Medical Health Science and Technology Project of Zhejiang Provincial Health Commission (no.2019KY453), by the Ningbo Natural Science Foundation (grant number 2015A610179), by Zhejiang Science and Technology Bureau (no. 2012C33126), the Zhejiang Provincial Natural Science Foundation of China (no. LY20H100003 and LY15H100004)and the Wenzhou Municipal Science and Technology Bureau (no. 2014Y0309). The authors have no other relevant affiliations or financial involvement with anyorganization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.No writing assistance was utilized in the production of this manuscript