LncRNA Gm12840 Mediates WISP1 to Regulate Ischemia-Reperfusion-Induced Renal Fibrosis by Sponging miR-677-5p

Abstract

Aim: We aimed to identify that long noncoding RNAs (lncRNAs) are involved in ischemia-reperfusion (IR)-induced late fibrosis of kidney and may constitute novel therapeutic strategies for acute kidney injury-induced chronic kidney disease. Materials & methods: We performed the mouse model of IR later induced renal fibrosis and analyzed lncRNA profiles using second-generation sequencing during the pathogenesis. Results: The expression levels of 43 lncRNAs and 141 lncRNAs were respectively changed significantly 7 days and 2 weeks after IR treatment. Based on the correlation analysis of the differentially expressed genes, the interaction networks of lncRNAs, miRNAs and mRNA were structured. Conclusion: LncRNA (Gm12840) could act as a sponge for miR-677-5p to mediate fibroblast activation induced by TGF-β1 via the WISP1/PKB (Akt) signaling pathway.

Keywords::

• fibrosis
• ischemia
• lncRNA
• renal
• reperfusion

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.tandfonline.com/doi/suppl/10.2217/epi-2020-0054

Financial & competing interest disclosure

This work was supported by grants from the National Natural Science Foundation of China (Grant Number 81860130, 82060130), Natural Science Foundation of Guangdong province (Grant Number 2019A1515011087), Guangzhou Science and Technology Project (Grant Number 202002030038 and 201804010068), Panyu Science and Technology Project of Guangzhou (Grant Number 2017-Z04-63). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No funded writing assistance was utilized in the production of this manuscript.

Data accessibility statement

The data used to support the findings of this study are available from the corresponding author upon request. The raw sequence datasets supporting the results of this study are available in the sequence read archive repository (http://www.ncbi.nlm.nih.gov/sra/) under the accession number SRP105463.

Ethical conduct of research

Rodent-related studies were approved by the Laboratory Animal Ethics Committee of Guangzhou Eighth People’s Hospital, Guangzhou Medical University.

Additional information

Funding

This work was supported by grants from the National Natural Science Foundation of China (Grant Number 81860130, 82060130), Natural Science Foundation of Guangdong province (Grant Number 2019A1515011087), Guangzhou Science and Technology Project (Grant Number 202002030038 and 201804010068), Panyu Science and Technology Project of Guangzhou (Grant Number 2017-Z04-63). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No funded writing assistance was utilized in the production of this manuscript.