Function of Glutaredoxin 3 (Grx3) in Oxidative Stress Response Caused by Iron Homeostasis Disorder in Candida Albicans

Abstract

Aim: Glutaredoxin is a conserved oxidoreductase in eukaryotes and prokaryotes. This study aimed to determine the role of Grx3 in cell survival, iron homeostasis and the oxidative stress response in Candida albicans. Materials & methods: A grx3Δ/Δ mutant was obtained using PCR-mediated homologs recombination. The function of Grx3 was investigated by a series of biochemical methods. Results: Deletion of GRX3 impaired growth and cell cycle, disturbance of iron homeostasis and activated the oxidative stress response. Furthermore, disruption of GRX3 caused oxidative damage and growth defects of C. albicans. Conclusion: Our findings provide new insights into the role of GRX3 in C. albicans.

Keywords::

• Candida albicans
• Grx3
• iron homeostasis
• oxidative stress response

Financial & competing interests disclosure

This study is supported by the National Natural Science Foundation of China (No. 31670146, 81471923 and 31400132), the Tianjin Research Program of Application Foundation and Advanced Technology 17JCZDJC333000) and the Fundamental Research Funds for the Central Universities. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

Additional information

Funding

This study is supported by the National Natural Science Foundation of China (No. 31670146, 81471923 and 31400132), the Tianjin Research Program of Application Foundation and Advanced Technology 17JCZDJC333000) and the Fundamental Research Funds for the Central Universities. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.