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Abstract
Aim: To validate an in vitro caries model and to evaluate an experimental mouthwash containing Croton doctoris essential oil. Materials & methods: To validate the experimental model, we used McBain medium and polymicrobial biofilms. The EOM (essential oil mouthwash) was tested using the validated model. Microbial composition (colony-forming unit/ml), acidogenicity, enamel demineralization (percentage of surface enamel hardness loss), cytotoxicity and essential oil composition were evaluated. Results: The model was validated with 0.5% sucrose, duration of 4 days and treatments twice per day. There were statistically significant differences between the EOM, the negative control and chlorhexidine mouthwash in colony-forming unit/ml and percentage of surface enamel hardness loss. Cytotoxicity was similar to that of chlorhexidine mouthwash. A total of 66.11% of the essential oil consists of sesquiterpenes. Conclusion: The experimental mouthwash showed antimicrobial activity against polymicrobial biofilms and reduced enamel demineralization.
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Supplementary data
To view the supplementary data that accompany this paper please visit the journal website at: www.tandfonline.com/doi/suppl/10.2217/fmb-2017-0209
Acknowledgements
The authors thank AA Sobrinho and ÁCB Delbem for assistance in plant collection, MS Cenci for the initial assistance on the experimental design of the study and the saliva donors.
Financial & competing interests disclosure
This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq (444195/2014-9 and 304383/2016-3), Internationalization Program of UNESP – PROINTER (2017/1583) and the São Paulo Research Foundation (FAPESP), grants #2013/12547-4 and 2008/04114-2. This manuscript is based on YEA master's thesis (Graduate Program in Dental Sciences, São Paulo State University (Unesp), School of Dentistry, Araraquara). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
No writing assistance was utilized in the production of this manuscript.
Ethical conduct of research
The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.