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Abstract
Aim: To compare test results obtained from a PCR assay for the National Cancer Institute (NCI) five loci criteria for detecting microsatellite instability (MSI) with those obtained from immunohistochemistry of mismatch repair and a five-mononucleotide site amplification system in East Asian patients with colorectal cancer. Patients & methods: A total of 245 East Asian patients with colorectal cancer were studied retrospectively at our institution. Results: The consistency of the NCI panel PCR method compared with detection of mismatch repair protein expression by immunohistochemistry was 0.898. High level MSI (MSI-H) status was correlated with the Tumor, Node, Metastasis stage, tumor location site, metastasis, tumor grade, mucinous histological type and BRAF-type mutations. Conclusion: The NCI panel PCR assay has excellent sensitivity and specificity for detecting MSI in an East Asian population.
Supplemental Material
Financial & competing interests disclosure
The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.
Writing assistance was provided by Content Ed Net, Shanghai Co. Ltd and paid for by the authors.
Ethical conduct of the research
Ethical approval for the study was obtained from the ethical committee of Shanghai Changhai Hospital (approval number: [2017] Research ethical approval No. 12).