Clinical significance of Notch pathway-associated microRNA-107 in pancreatic ductal adenocarcinoma

Abstract

Background & aim: MicroRNAs associated with the Notch pathway play a critical role in the progression of pancreatic carcinoma. Our aim was to study the clinical significance of miR-107 and NOTCH2 in pancreatic ductal adenocarcinoma (PDAC). Methods: The circulating miR-107 levels in PDAC and controls were determined by qPCR. NOTCH2 protein (target) expression in tissue of PDAC, periampullary carcinoma, chronic pancreatitis and normal pancreatic tissue was assessed by immunohistochemistry. Results: The circulating miR-107 levels were found to be significantly reduced in PDAC as compared with controls. Additionally, NOTCH2 protein expression was higher in PDAC tissue as compared with controls and was clinically associated with metastasis. Conclusion: Our findings demonstrate the utility of circulating miR-107 as a potential differentiating marker in PDAC.

Keywords::

• biomarker
• chronic pancreatitis
• diagnostic potential
• early diagnosis
• microRNAs
• miR-107
• NOTCH2
• Notch signaling
• PDAC
• potential marker

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.tandfonline.com/doi/suppl/10.2217/fon-2022-0356

Author contributions

Safoora Rashid: study concept and design; acquisition of data; analysis and interpretation of data; drafting of the manuscript. Sumaira Rashid: administrative, technical or material support. P Das: analysis and interpretation of data. N Singh: administrative, technical or material support. N Dash: administrative, technical or material support. B Nayak: administrative, technical or material support. H Sati: statistical analysis. S Gupta: study supervision; administrative, technical or material support. S Chauhan: critical revision of the manuscript for important intellectual content; study supervision. A Saraya: study concept and design; critical revision of the manuscript for important intellectual content; obtained funding; study supervision.

Financial competing interests disclosure

The study was supported by grants from the Department of Science and Technology, New Delhi. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

Additional information

Funding

The study was supported by grants from the Department of Science and Technology, New Delhi. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.