A Novel Torque Teno Mini Virus in the Blood of the Patient With Lymphoma

Abstract

Aim: To characterize a novel anellovirus and analyze the relationship of the novel anellovirus with other anelloviruses. Materials & methods: Twenty-one serum samples of lymphoma patients were collected and viral metagenomics method was used to identify the novel anellovirus; CLUSTALW1.8 and MEGA6.0 was used to perform phylogenetic analysis; prevalence investigation was performed by nest PCR. Results: A novel anellovirus was discovered from two patients with lymphoma. The complete genome of torque teno mini virus (TTMV)-SHA is 2880 nt in length. Phylogenetic analysis based on ORF1 indicated that TTMV-SHA clusters with EF538882 sharing 62.5% sequence similarity, and the sequences divergence is 54%. Conclusion: TTMV (SHA) belonged to a novel anellovirus species, whether this novel anellovirus is associated with lymphoma needs further studies.

Keywords: :

• anelloviruses
• amplification
• inverse primers
• lymphoma
• phylogenetic analysis
• prevalence investigation
• serum
• TTMV
• viral communities
• viral metagenomics

Financial & competing interests disclosure

This work was partly supported by the Taizhou Science and Technology Support (Social Development) Project Foundation no. TS201623, Taizhou People’s Hospital Foundation no. ZL201732 and Taizhou Science and Technology Support (Social Development) Project Foundation no. SSF20170218. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The study was approved by Taizhou medical ethics committee.