Comparison of Coxsackievirus A12 Genome Isolated From Patients With Different Symptoms in Yunnan, Southwest China

Abstract

Aim: Coxsackievirus A12 (CVA12) mainly causes hand–foot–mouth disease (HFMD) or herpangina (HA). Thus, the genomic characteristics of CVA12 isolates from Southwest China, especially the discrepancy between CVA12-HFMD and CVA12-HA were analyzed. Patients & methods: The full-length genome sequences of CVA12-HFMD and CVA12-HA were obtained and phylogenetic, nucleotide mutation, amino acid substitution and recombinant analyses were performed. Results: All CVA12 were closest to the Netherlands (2013) and have possibly recombined in the capsid coding region (P1) with other HEV-A. In the coding region, 54 base mutation result in 11 nonsynonymous mutations and four of them were identical mutations between CVA12-HFMD and CVA12-HA. Conclusion: Whether the presence of four consistent nonsynonymous mutation sites affect the virulence of the CVA12 deserves further study.

Keywords:

• CVA12
• evolutionary analysis
• hand–foot–mouth disease
• molecular characterization
• recombination analysis

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at:www.tandfonline.com/doi/full/10.2217/fvl-2020-0103

Author contributions

Q Rao contributed toward data curation and writing the original draft. Z Zhang contributed toward data processing. H Jiang contributed toward sample collection. M Wang, T Du and X Li contributed toward methodology. R Huang, M Fan and S Long contributed toward software. X Liu and T Zhang contributed toward resources, Q Sun contributed toward conceptualization, writing, review and editing.

Acknowledgments

The authors thanked C Zhao for his assistance in the process of experimental research. In addition, they thanked J Guan (Institute of medical biology, Chinese Academy of Medical Sciences) for his guidance on the use of the software.

Financial & competing interests disclosure

This work was supported by grants from the Foundation of the CAMS (Chinese Academy of Medical Sciences) Initiative for Innovative Medicine (CAMS-I2M) (grant no. 2017-I2M-2-006), the Foundation of the Yunnan Education Science and Technology Research (grant no. 2018JS241), the Natural Science Foundation of Yunnan Province (2016FA029), the Foundation of the Kunming Science and Technology Research (grant no. 2017-1-S-15381), Yunnan Health training Project of High level talents (no. D-2017053), Experts training Project for the Academy and Technology in Kunming City, and Yunnan Key Laboratory of Children’s Major Disease Research. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

This study was approved by the ethics committee of Kunming Children’s Hospital. Written consent was obtained from each participating patient’s parents or guardians in the study. The manuscript is an original work and has not been published or is under consideration for publication in another journal. The study complies with current ethical consideration. It has been confirmed that all the listed authors have participated actively in the study, and have seen and approved the submitted manuscript. The authors do not have any possible conflicts of interest.

Availability of data & materials

Data were obtained from our research and the NCBI database.