Mass Spectrometry-Based Proteomic Approaches for Discovery of HIV–Host Interactions

ABSTRACT 

A molecular understanding of viral infection requires a multi-disciplinary approach. Mass spectrometry has emerged as an indispensable tool to investigate the complex and dynamic interactions between HIV-1 and its host. It has been employed to study protein associations, changes in protein abundance and post-translational modifications occurring after viral infection. Here, we review and provide examples of mass spectrometry-based proteomic approaches currently used to explore virus–host interaction. Efforts in this area are certain to accelerate the discovery of the unique molecular strategies utilized by the virus to commandeer the cell as well as mechanisms of host defense.

KEYWORDS:

• affinity purification
• HIV-1
• MS-based proteomics
• post-translational modification
• quantitative MS
• virus–host interactions

Financial & competing interests disclosure

Support is acknowledged from the NIH (R01 AI081615 and R21 AI097233, both awarded to MA Muesing) to help defray costs associated with the preparation and publication of this manuscript. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Additional information

Funding

Support is acknowledged from the NIH (R01 AI081615 and R21 AI097233, both awarded to MA Muesing) to help defray costs associated with the preparation and publication of this manuscript. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.