Targeted Delivery of β-Glucosidase-Loaded Magnetic Nanoparticles: Effect of External Magnetic Field Duration and Intensity

Abstract

Aim: The effect of applied magnetic field duration and intensity on the delivery of β-glucosidase-loaded magnetic nanoparticles was evaluated. Materials & methods: The prepared β-glucosidase-loaded magnetic nanoparticles were targeted to subcutaneous tumors with an external magnetic field. Iron concentration and enzyme activity in tumor tissue were analyzed via electron spin resonance detection, Prussian blue staining and enzyme activity measurement. Results: The increase in magnetic nanoparticles quantity and enzyme activity in tumor tissue was not synchronous with the magnetic targeting duration. In addition, accumulation of magnetic nanoparticles and the increase in enzyme activity were not synchronous with the magnetic field intensity. Conclusion: The results suggested that appropriate magnetic field conditions should be considered for targeted delivery of bioactivity proteins based on magnetic nanoparticles.

Keywords::

• β-glucosidase
• enzyme activity
• magnetic field duration and intensity
• magnetic nanoparticles
• targeted delivery

Financial & competing interests disclosure

This work was financially supported by the National Natural Science Foundation of China (no. 81472410, 81160314) and the Natural Science Foundation of Hubei Province (no. 2016CFB524, 2017CFB484). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

Writing support was provided by American Journal Experts and was funded by the authors themselves.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations.

Acknowledgments

The authors deeply thank K Lv (College of Resources and Environmental Science, South-Central University for Nationalities, Wuhan, Hubei, China) for the superconducting quantum interference device measurements.

Additional information

Funding

This work was financially supported by the National Natural Science Foundation of China (no. 81472410, 81160314) and the Natural Science Foundation of Hubei Province (no. 2016CFB524, 2017CFB484). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.