Corrigendum

This article refers to:

Laser-Assisted Cell Printing: Principle, Physical Parameters Versus Cell Fate and Perspectives in Tissue Engineering

In the Technology Report by Fabien Guillemot, Agnès Souquet, Sylvain Catros and Bertrand Guillotin, Laser-assisted cell printing: principle, physical parameters versus cell fate and perspectives in tissue engineering (Nanomedicine 5[3], 507–515 [2010]), on page 511, some of the reference citations in Table 1 were incorrect. The table was originally printed as:

Table 1. Key milestones in the development of safe laser-assisted bioprinting procedures.

Laser-assisted cell printing method	Key milestones	Ref.
MAPLE-DW, 193 nm	Laser transfer by MAPLE-DW does not damage printed protein epitopes.	[52]
MAPLE-DW, 193 nm, 20 ns, 100 Hz	Transfer technique was found to be compatible with cell transfer as all transferred cells were found to be viable.	[30]
MAPLE-DW, 193 nm, 30 ns	When pluripotent embryonal carcinoma cells (P19) were printed onto a thick layer of Matrigel™ (> 40 µm), more than 90% of the cells survived the transfer process, remained viable and could differentiate into the neural or muscle cell lineage.	[44]
BioLP, 193 nm, 20 ns	A laser absorbing interlayer was placed between the incident laser pulse and biomaterials to be printed. Proof-of-principle experiments of multicolor printing and 3D cell pattern using BioLP (by spreading a 75 µm thick layer of Matrigel between each cell layer).	[28]
BioLP, 248 nm, 2 ns, 100 Hz	Number of cells per spot was shown to be determined by random sampling statistics. Demonstration of minimal expression of heat shock proteins by printed cells.	[53]
AFA-LIFT, 248 nm, 30 ns	Using time-resolved imaging, cell printing was shown to occur on a 1 µs time scale at a constant velocity with an estimated acceleration of 10^7 m/s^2.	[29]
MAPLE-DW, 193 nm, 10 Hz	Co-deposition of hydroxyapatite, MG 63 osteoblast-like cells and extracellular matrix demonstrated the possibility to print complex bioinks.	[54]
MAPLE-DW, 193 nm	Formation of a 3D neural network by transferring B35 neuronal cells to different depths in Matrigel by applying increasing laser fluences (energy per surface; J/m^2).	[55]
MAPLE-DW, 193 nm, 30 ns, 10 Hz, triazene layer	An intermediate layer of absorbing triazene polymer (dynamic release layer) was used to provide a gentler and more efficient printing (i.e., at lower fluences than usual) of B35 neuroblast cells.	[56]
LIFT, 800 nm, 120 fs, 1 kHz	Gap between NIH3T3 fibroblasts containing ribbon and substrate was filled by culture medium to avoid cell stress due to film drying.	[57]
MAPLE-DW, 355 nm, 15 ns	Mammalian embryonic stem cells were printed using a thick polyimide absorbing layer, which differs from a dynamic release layer.	[58]
BioLP, 266 nm, 5 ns, TiO2 (40 nm)	Glycerol was substituted with methyl cellulose to avoid ink dehydration without toxic effects.	[40]
MAPLE-DW, 93 nm, 300 Hz, triazene DRL	Single-cell printing and groups of cells (approximately 20–40 cells) were obtained when the ribbon was preliminary seeded with a lower cell concentration and near 100% confluence, respectively.	[59]
MAPLE-DW, 193 nm, 2 ns	Some of the MAPLE-DW process-induced damage to yeast cells was reversible and the post-transfer yeast cell recovery was a function of the laser fluence (85–1500 mJ/cm^2)	[43]
Laser-assisted bioprinting, 1064 nm, 5–10 kHz	High-throughout cell printing (up to tens of thousands of droplets per second) was demonstrated using an original workstation. EA.hy926 endothelial cells remained viable after printing using a near-IR nano second laser.	[41]

BioLP: Biological laser printe; DRL: Dynamic release layer; LIFT: Laser-induced forward transfer; MAPLE-DW: Matrix-assisted pulse laser evaporation-direct write.

The correct table is shown overleaf…

Table 1. Key milestones in the development of safe laser-assisted bioprinting procedures.

Laser-assisted cell printing method	Key milestones	Ref.
MAPLE-DW, 193 nm	Laser transfer by MAPLE-DW does not damage printed protein epitopes.	[52]
MAPLE-DW, 193 nm, 20 ns, 100 Hz	Transfer technique was found to be compatible with cell transfer as all transferred cells were found to be viable.	[32]
MAPLE-DW, 193 nm, 30 ns	When pluripotent embryonal carcinoma cells (P19) were printed onto a thick layer of Matrigel™ (> 40 µm), more than 90% of the cells survived the transfer process, remained viable and could differentiate into the neural or muscle cell lineage.	[46]
BioLP, 193 nm, 20 ns	A laser absorbing interlayer was placed between the incident laser pulse and biomaterials to be printed. Proof-of-principle experiments of multicolor printing and 3D cell pattern using BioLP (by spreading a 75 µm thick layer of Matrigel between each cell layer).	[30]
BioLP, 248 nm, 2 ns, 100 Hz	Number of cells per spot was shown to be determined by random sampling statistics. Demonstration of minimal expression of heat shock proteins by printed cells.	[53]
AFA-LIFT, 248 nm, 30 ns	Using time-resolved imaging, cell printing was shown to occur on a 1 µs time scale at a constant velocity with an estimated acceleration of 10^7 m/s^2.	[31]
MAPLE-DW, 193 nm, 10 Hz	Co-deposition of hydroxyapatite, MG 63 osteoblast-like cells and extracellular matrix demonstrated the possibility to print complex bioinks.	[54]
MAPLE-DW, 193 nm	Formation of a 3D neural network by transferring B35 neuronal cells to different depths in Matrigel by applying increasing laser fluences (energy per surface; J/m^2).	[55]
MAPLE-DW, 193 nm, 30 ns, 10 Hz, triazene layer	An intermediate layer of absorbing triazene polymer (dynamic release layer) was used to provide a gentler and more efficient printing (i.e., at lower fluences than usual) of B35 neuroblast cells.	[56]
LIFT, 800 nm, 120 fs, 1 kHz	Gap between NIH3T3 fibroblasts containing ribbon and substrate was filled by culture medium to avoid cell stress due to film drying.	[57]
MAPLE-DW, 355 nm, 15 ns	Mammalian embryonic stem cells were printed using a thick polyimide absorbing layer, which differs from a dynamic release layer.	[58]
BioLP, 266 nm, 5 ns, TiO2 (40 nm)	Glycerol was substituted with methyl cellulose to avoid ink dehydration without toxic effects.	[42]
MAPLE-DW, 93 nm, 300 Hz, triazene DRL	Single-cell printing and groups of cells (approximately 20–40 cells) were obtained when the ribbon was preliminary seeded with a lower cell concentration and near 100% confluence, respectively.	[59]
MAPLE-DW, 193 nm, 2 ns	Some of the MAPLE-DW process-induced damage to yeast cells was reversible and the post-transfer yeast cell recovery was a function of the laser fluence (85–1500 mJ/cm^2)	[44]
Laser-assisted bioprinting, 1064 nm, 5–10 kHz	High-throughout cell printing (up to tens of thousands of droplets per second) was demonstrated using an original workstation. EA.hy926 endothelial cells remained viable after printing using a near-IR nano second laser.	[43]

BioLP: Biological laser printe; DRL: Dynamic release layer; LIFT: Laser-induced forward transfer; MAPLE-DW: Matrix-assisted pulse laser evaporation-direct write.

The authors would like to sincerely apologize for any inconvenience or confusion this may have caused our readers.