Following publication of the Research Article by Jessica Schiavi, Laetitia Keller, David-Nicolas Morand, Natalia De Isla, Olivier Huck, Jean Christophe Lutz, Didier Mainard, Pascale Schwinté & Nadia Benkirane-Jessel, titled ‘Active implant combining human stem cell microtissues and growth factors for bone-regenerative nanomedicine’, which appeared in the March 2015 issue of Nanomedicine (Nanomedicine [Lond.]) 10[5], 753–763 [2015]), it has been brought to our attention that the first three paragraphs on page 755 were presented incorrectly as:
Chondrogenic differentiation
hMSCs were seeded as a pellet of 250,000 cells and cultured for 28 days with standard chondrogenic medium (Lonza, France) supplemented with 10 ng ml-1 of TGF-β3. Samples were then fixed with 4% formalde cartilage glycosaminoglycans.
Osteogenic differenti hyde and paraffin sections were obtained and stained with Alcian Blue staining to visualization.
hMSCs were seeded at 100 cells cm-2 and put in culture for 14 days with hMSCs medium. After that, we induced differentiation by supplementing standard culture medium with ascorbic acid 60 μm, β-glycerol phosphate 10 mm and dexamethasone 0.1 μm for
21 days. Cells were washed with PBS, fixed in ice-cold 70% ethanol and stained with Alizarin Red (pH: 4.1; Sigma-Aldrich, France) to detect Ca2+ deposits.
The correct presentation should be:
Chondrogenic differentiation
hMSCs were seeded as a pellet of 250,000 cells and cultured for 28 days with standard chondrogenic medium (Lonza, France) supplemented with 10 ng ml−1 of TGF-β3. Samples were then fixed with 4% formaldehyde and paraffin sections were obtained and stained with Alcian Blue staining to visualize cartilage glycosaminoglycans.
Osteogenic differentiation
hMSCs were seeded at 100 cells cm−2 and put in culture for 14 days with hMSCs medium. After that, we induced differentiation by supplementing standard culture medium with ascorbic acid 60 μm, β-glycerol phosphate 10 mm and dexamethasone 0.1 μm for 21 days. Cells were washed with PBS, fixed in ice-cold 70% ethanol and stained with Alizarin Red (pH: 4.1; Sigma-Aldrich, France) to detect Ca2+ deposits.
The editors of Nanomedicine would like to sincerely apologize for any inconvenience or confusion this may have caused our readers.