Noninvasive Subcellular Imaging in Breast Cancer Risk Assessment: Construction of Diagnostic Windows

Abstract

Aim: Breast cancer is the most common cause of cancer-related death among women. Delayed diagnosis leads to development of metastasis and impairs the outcome. This study was designed to utilize subcellular DNA imaging by ‘comet assay‘ and determine pathology-specific comet patterns as the robust biomarker to distinguish between high and low risk for breast cancer development among predisposed individuals with benign breast alterations. Materials & methods: A total of 161 patients were grouped as follows: benignancy, premenopause (n = 59); benignancy, postmenopause (n = 20); breast cancer, premenopause (n = 19); breast cancer, postmenopause (n = 63). On average, 800–1000 comets were evaluated per patient. Results & conclusion: The qualitative comet assay is an innovative approach for breast cancer risk assessment that can be utilized for the screening of highly predisposed individuals among patients with benign breast alterations. Pathology-specific comet patterns have been identified as the robust biomarker for breast cancer risk. Mathematic model-based diagnostic windows have been constructed for their clinical application.

KEYWORDS::

• benign breast alteration
• biomarker
• blood tests
• breast cancer
• comet assay
• personalized medicine
• predictive diagnostics
• risk assessment
• specific patterns

Acknowledgements

The authors thank Heike Mönkemann, PhD, for the collection of blood samples and Babitha George, MSc, for her technical assistance in ‘comet assay‘ analysis.

Financial & competing interests disclosure

The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.