Preclinical Study for the Treatment of Diabetes Mellitus Using β-like Cells Derived from Human Dental Pulp Stem Cells

Abstract

Aim: The current study assessed whether insulin-producing cells obtained from dental pulp stem cells (DPSCs) can be a new therapeutic approach in a rat model of diabetes mellitus (DM). Materials & methods: Stem cells were differentiated into pancreatic β cells under hydrogen sulfide exposure via 2D and 3D methods. Each β-like cell was immunostained and transplanted into DM rats, after which the in vivo therapeutic effect was determined. Results: Immunostaining revealed the expression of various β-cell markers in each β-like cell differentiated using the 3D method. DPSC-derived β-like cell differentiated via the 3D method promoted a sufficient therapeutic effect. Conclusion: The 3D method promoted islet differentiation, indicating that DPSC-derived β-like cell transplantation could be a new approach for DM treatment.

Keywords::

• dental pulp
• diabetes mellitus
• mesenchymal stem cells
• rats

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.tandfonline.com/doi/suppl/10.2217/rme-2022-0092

Author contributions

K Yaegaki substantial contributions to the conception or design of the work and the acquisition, analysis and interpretation of data for the work.

Acknowledgments

We would like to thank K Yaegaki for supervising this study. The authors would like to thank Enago (www.enago.jp) for the English language review.

Financial & competing interests disclosure

The study was supported by the Dental Pulp Stem Cell Grant from The Nippon Dental University, Tokyo, Japan. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations.

Additional information

Funding

Financial & competing interests disclosure The study was supported by the Dental Pulp Stem Cell Grant from The Nippon Dental University, Tokyo, Japan. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.