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The location of efferent vestibular neurons in the brainstem of newborn kittens was determined by means of horseradish peroxidase used as a tracer of retrograde protein transport. Two to three milligrams of horseradish peroxidase were injected into the vestibule of 8 kittens. After survival for 24 hours, the animals were fixed by perfusion and the brainstems sectioned. Stains for peroxidase demonstrated the labelled neurons lateral to the abducens nucleus bilaterally.
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